Wei Li
Chinese Academy of Sciences
12Publications
10H-index
1,906Citations
Publications 12
Newest
Published on Mar 1, 2014in Cell Research 15.39
Tang Hai13
Estimated H-index: 13
,
Fei Teng7
Estimated H-index: 7
+ 2 AuthorsQi Zhou41
Estimated H-index: 41
The pig is an important livestock for food supply and an ideal model for various human diseases. Efficient and precise genetic engineering in pigs holds great promise in agriculture and biomedicine1. Using currently available approach, generating specific gene modifications in pigs requires two steps. First, site-specific nucleases such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) are used to generate targeted mutations in pig somatic cells. Then t...
235 Citations Source Cite
Published on Feb 1, 2014in Cell 31.40
Yuyu Niu15
Estimated H-index: 15
,
Bin Shen14
Estimated H-index: 14
(National Resource Center)
+ 25 AuthorsWei Li10
Estimated H-index: 10
(Chinese Academy of Sciences)
Summary Monkeys serve as important model species for studying human diseases and developing therapeutic strategies, yet the application of monkeys in biomedical researches has been significantly hindered by the difficulties in producing animals genetically modified at the desired target sites. Here, we first applied the CRISPR/Cas9 system, a versatile tool for editing the genes of different organisms, to target monkey genomes. By coinjection of Cas9 mRNA and sgRNAs into one-cell-stage embryos, w...
566 Citations Source Cite
Published on Aug 1, 2013in Nature Biotechnology 35.72
Wei Li10
Estimated H-index: 10
,
Fei Teng7
Estimated H-index: 7
+ 1 AuthorsQi Zhou41
Estimated H-index: 41
Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems
276 Citations Source Cite
Published on Sep 20, 2013in Journal of Biological Chemistry 4.01
Minggui Jiang4
Estimated H-index: 4
,
Tianda Li7
Estimated H-index: 7
+ 10 AuthorsWei Li10
Estimated H-index: 10
The rat is an important animal model for human disease research. Using inhibitors of glycogen synthase kinase 3 and MAPK signaling pathways, rat embryonic stem cells and rat induced pluripotent stem cells (riPSCs) have been derived. However, unlike rat embryonic stem cells, germ line competent riPSCs have only been derived from Wistar rats at low efficiency. Here, we found that an optimized induction medium containing knock-out serum replacement and vitamin C improved the rate and efficiency of ...
7 Citations Source Cite
Published on Nov 1, 2013in Cell Research 15.39
Haifeng Wan11
Estimated H-index: 11
,
Zhengquan He3
Estimated H-index: 3
+ 14 AuthorsXin Li9
Estimated H-index: 9
19 Citations Source Cite
Published on Oct 1, 2013in Journal of Molecular Cell Biology 5.59
Wei Li10
Estimated H-index: 10
(Chinese Academy of Sciences),
Xuejiang Guo28
Estimated H-index: 28
(Nanjing Medical University)
+ 11 AuthorsYan Yuan6
Estimated H-index: 6
(Nanjing Medical University)
11 Citations Source Cite
Published on Oct 1, 2012in Nature 41.58
Wei Li10
Estimated H-index: 10
,
Ling Shuai6
Estimated H-index: 6
+ 17 AuthorsXin Li9
Estimated H-index: 9
Mouse androgenetic haploid embryonic stem cell lines can be established by transferring sperm into an enucleated oocyte; the cells maintain haploidy and stable growth over 30 passages, express pluripotent markers, are able to differentiate into all three germ layers, contribute to germlines of chimaeras when injected into blastocysts and can produce fertile progeny that carry genetic modifications to the next generation.
99 Citations Source Cite
Published on Mar 1, 2011in Cell Research 15.39
Wei Li10
Estimated H-index: 10
,
Xiaoyang Zhao20
Estimated H-index: 20
+ 6 AuthorsQi Zhou41
Estimated H-index: 41
iPS cells generated without c-Myc have active Dlk1-Dio3 region and are capable of producing full-term mice through tetraploid complementation
20 Citations Source Cite
Published on Sep 1, 2010in Stem Cell Reviews and Reports 3.61
Xiaoyang Zhao20
Estimated H-index: 20
(Chinese Academy of Sciences),
Wei Li10
Estimated H-index: 10
(Chinese Academy of Sciences)
+ 8 AuthorsFanyi Zeng11
Estimated H-index: 11
(Shanghai Jiao Tong University)
Previous studies demonstrated that induced pluripotent stem (iPS) cells could produce viable mice through tetraploid complementation, which was thought to be the most stringent test for pluripotency. However, these highly pluripotent iPS cells were previously reported to be generated from fibroblasts of embryonic origin. Achieving fully pluripotent iPS cells from multiple cell types, especially easily accessible adult tissues, will lead to a much greater clinical impact. We successfully generate...
34 Citations Source Cite
Published on Mar 1, 2010in Cell Research 15.39
Xiaoyang Zhao20
Estimated H-index: 20
,
Wei Li10
Estimated H-index: 10
+ 9 AuthorsLiu Wang21
Estimated H-index: 21
Efficient and rapid generation of induced pluripotent stem cells using an alternative culture medium
22 Citations Source Cite
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