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Xingxu Huang
National Resource Center
27Publications
19H-index
2,138Citations
Publications 27
Newest
#1Yuanwu Ma (Academy of Medical Sciences, United Kingdom)H-Index: 7
#2Lei Yu (Academy of Medical Sciences, United Kingdom)H-Index: 73
Last.Lianfeng Zhang (Academy of Medical Sciences, United Kingdom)H-Index: 7
view all 13 authors...
#1X. Wang (NWAFU: Northwest A&F University)H-Index: 8
#2Yiyuan Niu (NWAFU: Northwest A&F University)H-Index: 7
Last.Yulin Chen (NWAFU: Northwest A&F University)H-Index: 15
view all 11 authors...
#1Yuanwu Ma (Academy of Medical Sciences, United Kingdom)H-Index: 7
#2Lianfeng Zhang (Academy of Medical Sciences, United Kingdom)H-Index: 7
Last.Xingxu Huang (ShanghaiTech University)H-Index: 19
view all 3 authors...
#1Xiaolong Wang (NWAFU: Northwest A&F University)H-Index: 10
#2Bei Cai (NWAFU: Northwest A&F University)H-Index: 6
Last.Yulin Chen (NWAFU: Northwest A&F University)H-Index: 15
view all 16 authors...
Precision genetic engineering accelerates the genetic improvement of livestock for agriculture and biomedicine. We have recently reported our success in producing gene-modified goats using the CRISPR/Cas9 system through microinjection of Cas9 mRNA and sgRNAs targeting the MSTN and FGF5 genes in goat embryos. By investigating the influence of gene modification on the phenotypes of Cas9-mediated goats, we herein demonstrate that the utility of this approach involving the disruption of FGF5 results...
#1Yuanwu Ma (Academy of Medical Sciences, United Kingdom)H-Index: 7
#2Wei Chen (Academy of Medical Sciences, United Kingdom)H-Index: 9
Last.Lianfeng Zhang (Academy of Medical Sciences, United Kingdom)H-Index: 7
view all 9 authors...
ABSTRACTPrecise modifications such as site mutation, codon replacement, insertion or precise targeted deletion are needed for studies of accurate gene function. The CRISPR/Cas9 system has been proved as a powerful tool to generate gene knockout and knockin animals. But the homologous recombination (HR)-directed precise genetic modification mediated by CRISPR/Cas9 is relatively lower compared with nonhomologous end-joining (NHEJ) pathway and extremely expected to be improved. Here, in this study ...
#1Yu Kang (Kunming University of Science and Technology)H-Index: 8
#2Bo Zheng (Nanjing Medical University)H-Index: 9
Last.Xingxu Huang (ShanghaiTech University)H-Index: 19
view all 16 authors...
Mutations in the DAX1 locus cause X-linked adrenal hypoplasia congenita (AHC) and hypogonadotropic hypogonadism (HH), which manifest with primary adrenal insufficiency and incomplete or absent sexual maturation, respectively. The associated defects in spermatogenesis can range from spermatogenic arrest to Sertoli cell only syndrome. Conclusions from Dax1 knockout mouse models provide only limited insight into AHC/HH disease mechanisms, because mouse models exhibit more extensive abnormalities in...
#1Yan Yuan (Nanjing Medical University)H-Index: 6
#2Quan Zhou (Nanjing Medical University)H-Index: 5
Last.Xiaoyang Zhao (CAS: Chinese Academy of Sciences)H-Index: 22
view all 15 authors...
Genetic mutations could cause sperm deficiency, leading to male infertility. Without functional gametes in the testes, patients cannot produce progeny even with assisted reproduction technologies such as in vitro fertilization. It has been a major challenge to restore the fertility of gamete-deficient patients due to genetic mutations. In this study, using a Kitw/Kitwv mouse model, we investigated the feasibility of generating functional sperms from gamete-deficient mice by combining the reprogr...
#1Yongchang Chen (Kunming University of Science and Technology)H-Index: 13
#2Yiqiang Cui (Nanjing Medical University)H-Index: 11
Last.Xingxu Huang (National Resource Center)H-Index: 19
view all 12 authors...
#1Yuanwu Ma (Academy of Medical Sciences, United Kingdom)H-Index: 7
#2Lianfeng Zhang (Academy of Medical Sciences, United Kingdom)H-Index: 7
Last.Xingxu Huang (National Resource Center)H-Index: 19
view all 3 authors...
Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein (Cas)9-mediated genome modification enables us to edit the genomes of a variety of organisms rapidly and efficiently. The advantages of the CRISPR–Cas9 system have made it an increasingly popular genetic engineering tool for biological and therapeutic applications. Moreover, CRISPR–Cas9 has been employed to recruit functional domains that repress/activate gene expression or label specific genomic loci in...
#1Ranran ChengH-Index: 1
#2Jin Peng (Beijing University of Technology)H-Index: 1
Last.Pumin Zhang (BCM: Baylor College of Medicine)H-Index: 36
view all 13 authors...
Abstract We developed an adenovirus-based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue-specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus-based CRISPR/Cas9 system will find applications in a variety of experimental settings.
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