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Haley M. Brennan
Brookhaven National Laboratory
4Publications
Publications 4
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A technique is described for generating large well diffracting crystals from conditions that yield microcrystals. Crystallization using this technique is both rapid (crystals appear in <1 h) and robust (48 out of 48 co-crystallized with a fragment library, compared with 26 out of 48 using conventional hanging drop). Agarose gel is used to exclude nucleation inducing elements from the remaining crystallization cocktail. The chemicals in the crystallization cocktail are partitioned into high conce...
#1Yasmin N. Samara (UFSM: Universidade Federal de Santa Maria)H-Index: 1
#2Haley M. Brennan (W&M: College of William & Mary)
Last.Ludmila Leroy (UFMG: Universidade Federal de Minas Gerais)
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Crystal harvesting has proven to be difficult to automate and remains the rate-limiting step for many structure-determination and high-throughput screening projects. This has resulted in crystals being prepared more rapidly than they can be harvested for X-ray data collection. Fourth-generation synchrotrons will support extraordinarily rapid rates of data acquisition, putting further pressure on the crystal-harvesting bottleneck. Here, a simple solution is reported in which crystals can be acous...
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