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Bradley J. Toghill
University of Leicester
9Publications
4H-index
42Citations
Publications 9
Newest
#1R. Charles Coombes (Imperial College London)H-Index: 64
#2Karen Page (University of Leicester)H-Index: 11
Last.Justin Stebbing (Imperial College London)H-Index: 44
view all 39 authors...
Purpose: Up to 30% of breast cancer patients relapse after primary treatment. There are no sensitive and reliable tests to monitor these patients and detect distant metastases before overt recurrence. Here we demonstrate the use of personalized ctDNA profiling for detection of recurrence in breast cancer. Methods: Forty-nine primary breast cancer patients were recruited following surgery and adjuvant therapy. Plasma samples (n=208) were collected every 6 months for up to 4 years. Personalized as...
#1R. C. CoombesH-Index: 30
#2Anne C ArmstrongH-Index: 14
view all 25 authors...
Background: Many breast cancer patients relapse after primary treatment but there are no reliable tests to detect distant metastases before they become overt. Here we show earlier identification of recurring patients through a scalable personalised ctDNA analysis. The method is applicable to all patients, and not limited to hot-spot mutations typically detected by gene panels. Methods: Forty-nine non-metastatic breast cancer patients were recruited following surgery and adjuvant therapy. Plasma ...
#1Bradley J. Toghill (University of Leicester)H-Index: 4
#2Athanasios Saratzis (University of Leicester)H-Index: 11
Last.Matthew J. Bown (University of Leicester)H-Index: 34
view all 5 authors...
Abdominal aortic aneurysm (AAA) is a deadly cardiovascular disease characterised by the gradual, irreversible dilation of the abdominal aorta. AAA is a complex genetic disease but little is known about the role of epigenetics. Our objective was to determine if global DNA methylation and CpG-specific methylation at known AAA risk loci is associated with AAA, and the functional effects of methylation changes. We assessed global methylation in peripheral blood mononuclear cell DNA from 92 individua...
#1Jacqui A. Shaw (University of Leicester)H-Index: 21
#2Karen Page (University of Leicester)H-Index: 11
Last.Brenda M. Rosales (Imperial College London)H-Index: 2
view all 14 authors...
Successful early detection and intervention in cancer is key if we are to drastically improve cancer survivorship. Previous research by us and others has shown that copy number and mutational profiling of circulating cell-free DNA (cfDNA) can detect minimal residual disease and predict who will relapse after early stage breast cancer (Shaw et al. Genome Res 2012, doi: 10.1101, Garcia-Murillas et al. Sci Transl Med. 2015 doi: 10.1126). Here we used massively parallel sequencing with the Ion CHEF ...
#1Bradley J. Toghill (University of Leicester)H-Index: 4
#2Athanasios Saratzis (University of Leicester)H-Index: 11
Last.Matthew J. Bown (University of Leicester)H-Index: 34
view all 3 authors...
Abstract Atherosclerosis and abdominal aortic aneurysms (AAAs) are multifactorial and polygenic diseases with known environmental and genetic risk factors that contribute toward disease development. Atherosclerosis represents an important independent risk factor for AAA, as people with AAA often have atherosclerosis. Studies have shown that comorbidity is usually between ~25% and 55%, but it is still not fully known whether this association is causal or a result of common shared risk profiles. M...
#1Bradley J. Toghill (University of Leicester)H-Index: 4
#2Athanasios Saratzis (University of Leicester)H-Index: 11
Last.Matthew J. Bown (University of Leicester)H-Index: 34
view all 5 authors...
Aortic aneurysmal disease may affect the thoracic and abdominal segments, but the pathophysiology and genetic causal background differ significantly. Abdominal aortic aneurysms (AAAs) are multifactorial and polygenic, whereas thoracic aortic aneurysms and dissections (TAAD) are typically familial and/or associated with certain syndromes. Various genomic loci have been linked to both diseases. Syndromic and nonsyndromic TAADs are the result of large-effect gene mutations in genes controlling extr...
#1Harindra E. Amarasinghe (University of Leicester)H-Index: 3
#2Bradley J. Toghill (University of Leicester)H-Index: 4
Last.Eamonn B. Mallon (University of Leicester)H-Index: 20
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Methylation has previously been associated with allele specific expression in ants. Recently, we found methylation is important in worker reproduction in the bumblebee Bombus terrestris. Here we searched for allele specific expression in twelve genes associated with worker reproduction in bees. We found allele specific expression in Ecdysone 20 monooxygenase and IMP-L2-like. Although we were unable to confirm a genetic or epigenetic cause for this allele specific expression, the expression patte...
#1Bradley J. Toghill (University of Leicester)H-Index: 4
#2Athanasios Saratzis (University of Leicester)H-Index: 11
Last.Matthew J. Bown (University of Leicester)H-Index: 34
view all 6 authors...
Abstract Abdominal aortic aneurysm (AAA) is characterised by the chronic degradation and gradual, irreversible dilation of the abdominal aorta. Smoking, genetics, male sex and increased age are major factors associated with developing AAA. Rupture contributes to around 2% of deaths in all Caucasians over 65, and there is no pharmaco-therapeutic treatment. Methylation is an epigenetic modification to DNA, where a methyl group is added to a cytosine base 5′ to a guanine (CpG dinucleotide). Methyla...
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