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Neha Malhan
Environmental Molecular Sciences Laboratory
2Publications
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Publications 3
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One challenge associated with the discovery and development of monoclonal antibody (mAb) therapeutics is the de-termination of heavy chain and light chain pairing. Advances in MS instrumentation and MS/MS methods have greatly enhanced capabilities for the analysis of large intact proteins yielding much more detailed and accurate proteoform characterization. Consequently, direct interrogation of intact antibodies or F(ab’)2 and Fab fragments has the poten-tial to significantly streamline therapeu...
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#1Mowei Zhou (Environmental Molecular Sciences Laboratory)H-Index: 12
#2Neha Malhan (Environmental Molecular Sciences Laboratory)H-Index: 1
Last.Ljiljana Paša-Tolić (Environmental Molecular Sciences Laboratory)H-Index: 42
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Abstract Sorghum [Sorghum bicolor (L.) Moench] is an important cereal crop noted for its ability to survive water-limiting conditions. Herein, we present an analytical workflow to explore the changes in histone modifications through plant developmental stages and two drought stresses in two sorghum genotypes that differ in their response to drought. Top-down mass spectrometry (MS) is an ideal method to profile histone modifications and distinguish closely related histone proteoforms. We analyzed...
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#1Jared B. Shaw (Environmental Molecular Sciences Laboratory)H-Index: 12
#2Neha Malhan (Environmental Molecular Sciences Laboratory)H-Index: 1
Last.Valery G. Voinov (Linus Pauling Institute)H-Index: 1
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Compared to traditional collision induced dissociation methods, electron capture dissociation (ECD) provides more comprehensive characterization of large peptides and proteins as well as preserves labile post-translational modifications. However, ECD experiments are generally restricted to the high magnetic fields of FTICR-MS that enable the reaction of large polycations and electrons. Here, we demonstrate the use of an electromagnetostatic ECD cell to perform ECD and hybrid ECD methods utilizin...
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