Ru Zhang
Carnegie Institution for Science
12Publications
7H-index
393Citations
Publications 12
Newest
Published on Mar 18, 2019in Nature Genetics 27.13
Xiaobo Li (Princeton University), Weronika Patena3
Estimated H-index: 3
(Princeton University)
+ 17 AuthorsRu Zhang7
Estimated H-index: 7
(Carnegie Institution for Science)
Photosynthetic organisms provide food and energy for nearly all life on Earth, yet half of their protein-coding genes remain uncharacterized1,2. Characterization of these genes could be greatly accelerated by new genetic resources for unicellular organisms. Here we generated a genome-wide, indexed library of mapped insertion mutants for the unicellular alga Chlamydomonas reinhardtii. The 62,389 mutants in the library, covering 83% of nuclear protein-coding genes, are available to the community. ...
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Published on Nov 7, 2018in bioRxiv
Xiaobo Li (Princeton University), Weronika Patena3
Estimated H-index: 3
(Princeton University)
+ 17 AuthorsRu Zhang7
Estimated H-index: 7
(Carnegie Institution for Science)
Photosynthetic organisms provide food and energy for nearly all life on Earth, yet half of their protein-coding genes remain uncharacterized. Characterization of these genes could be greatly accelerated by new genetic resources for unicellular organisms that complement the use of multicellular plants by enabling higher-throughput studies. Here, we generated a genome-wide, indexed library of mapped insertion mutants for the flagship unicellular alga Chlamydomonas reinhardtii (Chlamydomonas hereaf...
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Published on Apr 1, 2017in Plant Journal 5.78
Ru Zhang7
Estimated H-index: 7
(Carnegie Institution for Science),
Eva C.M. Nowack12
Estimated H-index: 12
(University of Düsseldorf)
+ 2 AuthorsArthur R. Grossman80
Estimated H-index: 80
(Carnegie Institution for Science)
Summary Plastid evolution has been attributed to a single primary endosymbiotic event that occurred about 1.6 billion years ago (BYA) in which a cyanobacterium was engulfed and retained by a eukaryotic cell, although early steps in plastid integration are poorly understood. The photosynthetic amoeba Paulinella chromatophora represents a unique model for the study of plastid evolution because it contains cyanobacterium-derived photosynthetic organelles termed ‘chromatophores’ that originated rela...
2 Citations Source Cite
Published on Feb 1, 2016in The Plant Cell 8.23
Xiaobo Li8
Estimated H-index: 8
(Carnegie Institution for Science),
Ru Zhang7
Estimated H-index: 7
(Carnegie Institution for Science)
+ 9 AuthorsSorel Fitz-Gibbon26
Estimated H-index: 26
(University of California, Los Angeles)
The green alga Chlamydomonas reinhardtii is a leading unicellular model for dissecting biological processes in photosynthetic eukaryotes. However, its usefulness has been limited by difficulties in obtaining mutants in specific genes of interest. To allow generation of large numbers of mapped mutants, we developed high-throughput methods that (1) enable easy maintenance of tens of thousands of Chlamydomonas strains by propagation on agar media and by cryogenic storage, (2) identify mutagenic ins...
80 Citations Source Cite
Published on Apr 1, 2014in The Plant Cell 8.23
Ru Zhang7
Estimated H-index: 7
(Carnegie Institution for Science),
Weronika Patena3
Estimated H-index: 3
(Carnegie Institution for Science)
+ 3 AuthorsMartin C. Jonikas16
Estimated H-index: 16
(Carnegie Institution for Science)
A high-throughput genetic screening platform in a single-celled photosynthetic eukaryote would be a transformative addition to the plant biology toolbox. Here, we present ChlaMmeSeq ( Chlamydomonas Mme I-based insertion site Sequencing), a tool for simultaneous mapping of tens of thousands of mutagenic insertion sites in the eukaryotic unicellular green alga Chlamydomonas reinhardtii . We first validated ChlaMmeSeq by in-depth characterization of individual insertion sites. We then applied ChlaM...
68 Citations Source Cite
Published on Sep 1, 2011in Photosynthesis Research 3.09
Ru Zhang7
Estimated H-index: 7
(Carnegie Institution for Science),
David M. Kramer49
Estimated H-index: 49
(Michigan State University)
+ 2 AuthorsThomas D. Sharkey73
Estimated H-index: 73
(Michigan State University)
Moderately high temperature reduces photosynthetic capacities of leaves with large effects on thylakoid reactions of photosynthesis, including xanthophyll conversion in the lipid phase of the thylakoid membrane. In previous studies, we have found that leaf temperature of 40°C increased zeaxanthin accumulation in dark-adapted, intact tobacco leaves following a brief illumination, but did not change the amount of zeaxanthin in light-adatped leaves. To investigate heat effects on zeaxanthin accumul...
8 Citations Source Cite
Published on Aug 1, 2010in Photosynthesis Research 3.09
Ru Zhang7
Estimated H-index: 7
(University of Wisconsin-Madison),
Robert R. Wise22
Estimated H-index: 22
(University of Wisconsin–Oshkosh)
+ 1 AuthorsThomas D. Sharkey73
Estimated H-index: 73
(Michigan State University)
Photosynthesis is inhibited by heat stress. This inhibition is rapidly reversible when heat stress is moderate but irreversible at higher temperature. Absorbance changes can be used to detect a variety of biophysical parameters in intact leaves. We found that moderate heat stress caused a large reduction of the apparent absorbance of green light in light-adapted, intact Arabidopsis thaliana leaves. Three mechanisms that can affect green light absorbance of leaves, namely, zeaxanthin accumulation...
35 Citations Source Cite
Published on Jul 15, 2010in Journal of Integrative Plant Biology 3.09
Thomas D. Sharkey73
Estimated H-index: 73
(Michigan State University),
Ru Zhang7
Estimated H-index: 7
(Carnegie Institution for Science)
Photosynthesis is sensitive to high temperature with reversible declines during moderate stress and irreversible damage with more severe stress. While many studies have focused on the irreversible damage, the reversible changes can tell how photosynthesis tolerates high temperature. Knowing how high temperature is tolerated could lead to ways of extending high temperature tolerance. New analytical methods have been used to probe electron and proton circuits of intact leaves at high temperature. ...
71 Citations Source Cite
Published on Nov 1, 2009in Plant Cell and Environment 5.42
Ru Zhang7
Estimated H-index: 7
(University of Wisconsin-Madison),
Jeffrey A. Cruz23
Estimated H-index: 23
(Washington State University)
+ 3 AuthorsThomas D. Sharkey73
Estimated H-index: 73
(Michigan State University)
We measured the ΔΨ and ΔpH components of the transthylakoid proton motive force (pmf) in light-adapted, intact tobacco leaves in response to moderate heat. The ΔΨ causes an electrochromic shift (ECS) in carotenoid absorbance spectra. The light–dark difference spectrum has a peak at 518 nm and the two components of the pmf were separated by following the ECS for 25 s after turning the light off. The ECS signal was deconvoluted by subtracting the effects of zeaxanthin formation (peak at 505 nm) an...
39 Citations Source Cite
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