Justin Jordaan
Rhodes University
EnzymeChemistryBiocatalysisNucleosideImmobilized enzyme
What is this?
Publications 3
#1Jon Del Arco (UEM: European University of Madrid)H-Index: 4
#2Justin JordaanH-Index: 2
Last. Jesús Fernández-Lucas (Grupo México)H-Index: 12
view all 4 authors...
Abstract The present work aims to develop a magnetic biocatalyst for customized production of nucleoside analogues using mutant His-tagged purine 2’-deoxyribosyltransferase from Trypanosoma brucei ( Tb PDT V11S ) immobilized onto Ni 2+ chelate magnetic iron oxide porous microparticles (M Tb PDT V11S ). Biochemical characterization revealed M Tb PDT V11S 5 as optimal candidate for further studies (10,552 IU g -1 ; retained activity 54 % at 50 °C and pH 6.5). Interestingly, M Tb PDT V11S 5 display...
#1Elena Pérez (UEM: European University of Madrid)H-Index: 2
#2Pedro A. Sánchez-Murcia (University of Vienna)H-Index: 8
Last. Jesús Fernández-Lucas (Grupo México)H-Index: 12
view all 7 authors...
5 CitationsSource
#1Jon Del ArcoH-Index: 4
Last. Jesús Fernández-LucasH-Index: 12
view all 8 authors...
The enzymatic synthesis of nucleotides offers several advantages over traditional multistep chemical methods, such as stereoselectivity, regioselectivity, enantioselectivity, simple downstream processing, and the use of mild reaction conditions. However, in order to scale up these bioprocesses, several drawbacks, such as the low enzyme stability and recycling, must be considered. Enzyme immobilization may overcome these cost-related problems by enhancing protein stability and facilitating the se...
5 CitationsSource