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Joost Schimmel
Leiden University
1Publications
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32Citations
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#1Alexander Zelensky (EUR: Erasmus University Rotterdam)H-Index: 4
#2Joost Schimmel (LEI: Leiden University)H-Index: 1
Last.Marcel Tijsterman (LEI: Leiden University)H-Index: 31
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Off-target or random integration of exogenous DNA hampers precise genomic engineering and presents a safety risk in clinical gene therapy strategies. Genetic definition of random integration has been lacking for decades. Here, we show that the A-family DNA polymerase θ (Pol θ) promotes random integration, while canonical non-homologous DNA end joining plays a secondary role; cells double deficient for polymerase θ and canonical non-homologous DNA end joining are devoid of any integration events,...
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