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Joost Schimmel
Leiden University
1Publications
1H-index
20Citations
Publications 1
Newest
Published on Dec 1, 2017in Nature Communications11.88
Alexander Zelensky4
Estimated H-index: 4
(EUR: Erasmus University Rotterdam),
Joost Schimmel1
Estimated H-index: 1
(LEI: Leiden University)
+ 2 AuthorsMarcel Tijsterman30
Estimated H-index: 30
(LEI: Leiden University)
Off-target or random integration of exogenous DNA hampers precise genomic engineering and presents a safety risk in clinical gene therapy strategies. Genetic definition of random integration has been lacking for decades. Here, we show that the A-family DNA polymerase θ (Pol θ) promotes random integration, while canonical non-homologous DNA end joining plays a secondary role; cells double deficient for polymerase θ and canonical non-homologous DNA end joining are devoid of any integration events,...
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