Jamie R. Wallen
Western Carolina University
Publications 2
#1Brittni M. FosterH-Index: 1
Last.J.R WallenH-Index: 4
view all 9 authors...
Recent structural studies of the bacteriophage T7 DNA replication system have shed light on how multiple proteins assemble to copy two antiparallel DNA strands. In T7, acidic C-terminal tails of both the primase-helicase and single-stranded DNA binding protein bind to two basic patches on the DNA polymerase to aid in replisome assembly, processivity, and coordinated DNA synthesis. Although these electrostatic interactions are essential for DNA replication, the molecular details for how these tai...
#1Jamie R. Wallen (WCU: Western Carolina University)H-Index: 1
#2Hao F. Zhang (WashU: Washington University in St. Louis)H-Index: 44
Last.Tom Ellenberger (WashU: Washington University in St. Louis)H-Index: 17
view all 10 authors...
Summary The physical organization of DNA enzymes at a replication fork enables efficient copying of two antiparallel DNA strands, yet dynamic protein interactions within the replication complex complicate replisome structural studies. We employed a combination of crystallographic, native mass spectrometry and small-angle X-ray scattering experiments to capture alternative structures of a model replication system encoded by bacteriophage T7. Two molecules of DNA polymerase bind the ring-shaped pr...
8 CitationsSource