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Ying Liu
University of Pittsburgh
Growth hormone receptorMolecular biologyJanus kinase 2Signal transductionBiology
5Publications
4H-index
38Citations
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Publications 5
Newest
#1Yue Zhang (UAB: University of Alabama at Birmingham)H-Index: 15
#2Sajina Gc (UAB: University of Alabama at Birmingham)H-Index: 1
Last. Stuart J. Frank (UAB: University of Alabama at Birmingham)H-Index: 45
view all 8 authors...
Abstract Human growth hormone (GH) binds and activates GH receptor (GHR) and prolactin (PRL) receptor (PRLR). LNCaP human prostate cancer cells express only GHR. A soluble fragment of IGF-1 receptor (IGF-1R) extracellular domain (sol IGF-1R) interacts with GHR and blocks GH signaling. We now explore sol IGF-1R's specificity for inhibiting GH signaling via GHR vs. PRLR and test GHR and PRLR extracellular domain inhibition determinants. Although T47D human breast cancer cells express GHR and PRLR,...
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#1Ying Liu (UAB: University of Alabama at Birmingham)H-Index: 4
#2Jing Jiang (UAB: University of Alabama at Birmingham)H-Index: 28
Last. Stuart J. Frank (UAB: University of Alabama at Birmingham)H-Index: 45
view all 6 authors...
: Growth hormone receptor (GHR) and prolactin (PRL) receptor (PRLR) are homologous transmembrane class I cytokine receptors. In humans, GH interacts with GHR homodimers or PRLR homodimers and PRL interacts with only PRLR homodimers to promote signaling. In human breast cancer cells endogenously expressing both receptors, GHR and PRLR specifically coimmunoprecipitate. We previously devised a split luciferase complementation assay to study GHR and PRLR assemblages. In this technique, firefly lucif...
4 CitationsSource
#1Ying Liu (Stanford University)H-Index: 4
#2Yue Zhang (Stanford University)H-Index: 15
Last. Stuart J. Frank (UAB: University of Alabama at Birmingham)H-Index: 45
view all 9 authors...
GH receptor (GHR) and prolactin (PRL) receptor (PRLR) are homologous transmembrane cytokine receptors. Each prehomodimerizes and ligand binding activates Janus Kinase 2 (JAK2)-signal transducer and activator of transcription (STAT) signaling pathways by inducing conformational changes within receptor homodimers. In humans, GHR is activated by GH, whereas PRLR is activated by both GH and PRL. We previously devised a split luciferase complementation assay, in which 1 receptor is fused to an N-term...
4 CitationsSource
#1Yujun Gan (UAB: University of Alabama at Birmingham)H-Index: 6
#2Ashiya Buckels (UAB: University of Alabama at Birmingham)H-Index: 2
Last. Stuart J. Frank (UAB: University of Alabama at Birmingham)H-Index: 45
view all 8 authors...
GH signaling yields multiple anabolic and metabolic effects. GH binds the transmembrane GH receptor (GHR) to activate the intracellular GHR-associated tyrosine kinase, Janus kinase 2 (JAK2), and downstream signals, including signal transducer and activator of transcription 5 (STAT5) activation and IGF-1 gene expression. Some GH effects are partly mediated by GH-induced IGF-1 via IGF-1 receptor (IGF-1R), a tyrosine kinase receptor. We previously demonstrated in non-human cells that GH causes form...
16 CitationsSource
#1Ying Liu (UAB: University of Alabama at Birmingham)H-Index: 4
#2Philip A. Berry (UAB: University of Alabama at Birmingham)H-Index: 2
Last. Stuart J. FrankH-Index: 45
view all 10 authors...
The transmembrane GH receptor (GHR) exists at least in part as a preformed homodimer on the cell surface. Structural and biochemical studies suggest that GH binds GHR in a 1:2 stoichiometry to effect acute GHR conformational changes that trigger the activation of the receptor-associated tyrosine kinase, Janus kinase 2 (JAK2), and downstream signaling. Despite information about GHR-GHR association derived from elegant fluorescence resonance energy transfer/bioluminescence resonance energy transfe...
9 CitationsSource
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