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Kiho Lee
Virginia Tech
25Publications
6H-index
74Citations
Publications 25
Newest
#1Kayla Carey (VT: Virginia Tech)
#2Junghyun Ryu (VT: Virginia Tech)H-Index: 5
Last.Kiho Lee (VT: Virginia Tech)H-Index: 6
view all 7 authors...
Background The CRISPR/Cas9 system can effectively introduce site-specific modifications to the genome. The efficiency is high enough to induce targeted genome modifications during embryogenesis, thus increasing the efficiency of producing genetically modified animal models and having potential clinical applications as an assisted reproductive technology. Because most of the CRISPR/Cas9 systems introduce site-specific double-stranded breaks (DSBs) to induce site-specific modifications, a major co...
#1Susan E. Duncan (VT: Virginia Tech)H-Index: 28
#2Robert Reinhard (VT: Virginia Tech)
Last.Randall S. Murch (VT: Virginia Tech)H-Index: 8
view all 10 authors...
Our national data and infrastructure security issues affecting the ‘bioeconomy’ are evolving rapidly. Simultaneously, the conversation about cybersecurity of the U.S. food and agricultural system (cyberbiosecurity) is incomplete and disjointed. The food and agricultural production sectors influence over 20% of the nation’s economy ($6.7T) and 15% of U.S. employment (43.3M jobs). The food and agricultural sectors are immensely diverse and they require advanced technologies and efficiencies that r...
#1K. Uh (VT: Virginia Tech)
#2Junghyun Ryu (VT: Virginia Tech)H-Index: 5
Last.Kiho Lee (VT: Virginia Tech)H-Index: 6
view all 6 authors...
Abstract Artificial oocyte activation is an essential step in somatic cell nuclear transfer (SCNT) and can enhance viability of embryos as a form of assisted reproductive technology (ART) in clinics. Most artificial activation methods have been developed to increase cytosolic calcium (Ca 2+ ) level in oocytes. Interestingly, recent studies have demonstrated that mammalian oocytes can be activated using N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN), a Zn 2+ chelator. Although effectiv...
#1K. Carey (VT: Virginia Tech)
#2K. Uh (VT: Virginia Tech)
Last.Kiho Lee (VT: Virginia Tech)H-Index: 6
view all 4 authors...
Despite extensive efforts, cellular reprogramming in livestock species has had limited success. Induced pluripotent stem cells (iPSC) have been established; however, these cells often show incomplete reprogramming status, and constitutive expression of exogenous reprogramming factors is required due to inactivation of endogenous pluripotency-related genes. A previous study reported that overexpression of the Xenopus egg-derived WAVE1 gene assists reprogramming of murine somatic cells into the pl...
#1K. Uh (VT: Virginia Tech)
#2Junghyun Ryu (VT: Virginia Tech)H-Index: 5
Last.Kiho Lee (VT: Virginia Tech)H-Index: 6
view all 5 authors...
Ten-eleven translocation (TET) methylcytosine dioxygenases are considered to play an important role in regulation of DNA methylation patterns by converting 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). TET3 protein, a member of TET family, is enriched in mature oocytes and early stage embryos and contributes to DNA demethylation of the paternal genome in zygotes. N-terminal CXXC domain of TET3 is thought to be important in catalysing 5mC oxidation through its DNA binding potential. H...
#1C. L. Timlin (VT: Virginia Tech)H-Index: 1
#2A. Lynn (VT: Virginia Tech)
Last.V. R. G. Mercadante (VT: Virginia Tech)H-Index: 3
view all 5 authors...
There is a continual search for reliable, noninvasive methods of selecting viable oocytes and embryos. Previous studies have indicated that the physical size of oocytes may reflect their developmental potential. The objective of this study was to observe the correlation between an oocyte’s diameter [including zona pellucida (ZP), cell area, and ZP thickness] and its ability to develop. Bovine cumulus-oocyte complexes were collected from abattoir-derived ovaries and incubated for 24 h in TCM-199-...
#1Junghyun Ryu (VT: Virginia Tech)H-Index: 5
#2Randall S. Prather (MU: University of Missouri)H-Index: 64
Last.Kiho Lee (VT: Virginia Tech)H-Index: 6
view all 3 authors...
Pigs are an important resource in agriculture and serve as a model for human diseases. Due to their physiological and anatomical similarities with humans, pigs can recapitulate symptoms of human diseases, making them a useful model in biomedicine. However, in the past pig models have not been widely used partially because of the difficulty in genetic modification. The lack of true embryonic stem cells in pigs forced researchers to utilize genetic modification in somatic cells and somatic cell nu...
#1Bumrae ChoH-Index: 19
#2Su Jin KimH-Index: 25
Last.Jung-Taek KangH-Index: 13
view all 8 authors...
Diabetes mellitus is a chronic disease with accompanying severe complications. Various animal models, mostly rodents due to availability of genetically modified lines, have been used to investigate the pathophysiology of diabetes. Using pigs for diabetic research can be beneficial because of their similarity in size, pathogenesis pathway, physiology, and metabolism with human. However, the use of pigs for diabetes research has been hampered due to only few pig models presenting diabetes symptoms...
#1C. L. Timlin (VT: Virginia Tech)
#2K. Uh (VT: Virginia Tech)
Last.Kiho Lee (VT: Virginia Tech)H-Index: 6
view all 4 authors...
Traditionally, artificial oocyte activation has been induced by stimulating intracellular calcium increase in the oocyte. Recently, the use of zinc chelators has also shown to be effective in inducing activation by decreasing intracellular concentration of zinc, mimicking events during fertilization; however, this has not been demonstrated in bovine oocytes. The use of artificial activation in bovine has potential for overcoming subfertility-related production loss and aid in livestock cloning. ...
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