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Kiho Lee
Virginia Tech
26Publications
7H-index
101Citations
Publications 31
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#1Kyungjun Uh (VT: Virginia Tech)H-Index: 1
#2N. Wax (VT: Virginia Tech)
Last.Kiho Lee (VT: Virginia Tech)H-Index: 7
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Fertilized oocytes undergo genome-wide DNA demethylation with the exception of imprinted genes and certain repetitive elements. Ten-eleven translocation 3 (TET3) protein has been known to be responsible for the DNA demethylation process by catalysing oxidation of 5-methylcytosine. Recent studies in the mouse indicated that multiple Tet3 isoforms exist in oocytes, implying differential actions of the isoforms. Previously, we reported the sequence of TET3 in porcine oocytes (GenBank: KC137685). He...
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#1Kiho Lee (VT: Virginia Tech)H-Index: 7
#2Kayla Farrell (VT: Virginia Tech)
Last.Kyungjun Uh (VT: Virginia Tech)H-Index: 1
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Traditionally, genetic engineering in the pig was a challenging task. Genetic engineering of somatic cells followed by somatic cell nuclear transfer (SCNT) could produce genetically engineered (GE) pigs carrying site-specific modifications. However, due to difficulties in engineering the genome of somatic cells and developmental defects associated with SCNT, a limited number of GE pig models were reported. Recent developments in genome-editing tools, such as zinc finger nucleases (ZFNs), transcr...
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#1Kiho Lee (VT: Virginia Tech)H-Index: 7
#2Kyungjun Uh (VT: Virginia Tech)H-Index: 1
Last.Kayla Farrell (VT: Virginia Tech)
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Abstract Historically, genetic engineering in livestock proved to be challenging. Without stable embryonic stem cell lines to utilize, somatic cell nuclear transfer (SCNT) had to be employed to produce many of the genetically engineered (GE) livestock models. Through the genetic engineering of somatic cells followed by SCNT, GE livestock models could be generated carrying site-specific modifications. Although successful, only a few GE livestock models were generated because of low efficiency and...
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#1Kayla Farrell (VT: Virginia Tech)
#2Kyungjun Uh (VT: Virginia Tech)H-Index: 1
Last.Kiho Lee (VT: Virginia Tech)H-Index: 7
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Establishing proper levels of pluripotency is essential for normal development. The genome of gametes is remodelled upon fertilisation and pluripotency-related genes are expressed in blastocysts. Multiple pluripotency-related genes are involved in the well-orchestrated process; however, detailed mechanistic actions remain elusive. The PRDM family genes are reported to be closely related to the pluripotency. A previous report noted that PRDM14 plays an important role in the maintenance of pluripo...
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#1Claire L Timlin (VT: Virginia Tech)H-Index: 1
#2A. Lynn (VT: Virginia Tech)
Last.V. R. G. Mercadante (VT: Virginia Tech)H-Index: 3
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The objective of this study was to determine if physical parameters of bovine zygotes are correlated with in vitro development success. We examined the relationship between a zygote's outer diameter (OD), cell area, and zona pellucida (ZP) thickness on blastocyst development and blastomere number. Bovine cumulus-oocyte complexes, from abattoir-derived ovaries, were matured in tissue culture medium 199-based maturation medium for 21-23 h. In vitro fertilisation was performed with frozen thawed se...
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#1Kayla Carey (VT: Virginia Tech)H-Index: 1
#2Junghyun Ryu (VT: Virginia Tech)H-Index: 6
Last.Kiho Lee (VT: Virginia Tech)H-Index: 7
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Background The CRISPR/Cas9 system can effectively introduce site-specific modifications to the genome. The efficiency is high enough to induce targeted genome modifications during embryogenesis, thus increasing the efficiency of producing genetically modified animal models and having potential clinical applications as an assisted reproductive technology. Because most of the CRISPR/Cas9 systems introduce site-specific double-stranded breaks (DSBs) to induce site-specific modifications, a major co...
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#1Susan E. Duncan (VT: Virginia Tech)H-Index: 29
#2Robert Reinhard (VT: Virginia Tech)
Last.Randall S. Murch (VT: Virginia Tech)H-Index: 10
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Our national data and infrastructure security issues affecting the ‘bioeconomy’ are evolving rapidly. Simultaneously, the conversation about cybersecurity of the U.S. food and agricultural system (cyberbiosecurity) is incomplete and disjointed. The food and agricultural production sectors influence over 20% of the nation’s economy ($6.7T) and 15% of U.S. employment (43.3M jobs). The food and agricultural sectors are immensely diverse and they require advanced technologies and efficiencies that r...
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#1Kyungjun Uh (VT: Virginia Tech)H-Index: 1
#2Junghyun Ryu (VT: Virginia Tech)H-Index: 6
Last.Kiho Lee (VT: Virginia Tech)H-Index: 7
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Abstract Artificial oocyte activation is an essential step in somatic cell nuclear transfer (SCNT) and can enhance viability of embryos as a form of assisted reproductive technology (ART) in clinics. Most artificial activation methods have been developed to increase cytosolic calcium (Ca2+) level in oocytes. Interestingly, recent studies have demonstrated that mammalian oocytes can be activated using N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN), a Zn2+ chelator. Although effective, ...
2 CitationsSource
#1K. Carey (VT: Virginia Tech)
#2Kyungjun Uh (VT: Virginia Tech)H-Index: 1
Last.Kiho Lee (VT: Virginia Tech)H-Index: 7
view all 4 authors...
Despite extensive efforts, cellular reprogramming in livestock species has had limited success. Induced pluripotent stem cells (iPSC) have been established; however, these cells often show incomplete reprogramming status, and constitutive expression of exogenous reprogramming factors is required due to inactivation of endogenous pluripotency-related genes. A previous study reported that overexpression of the Xenopus egg-derived WAVE1 gene assists reprogramming of murine somatic cells into the pl...
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