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Roland Preece
Cas9Genome editingT cellBiologyCRISPR
4Publications
2H-index
21Citations
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Publications 6
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#1Roland PreeceH-Index: 2
#2C GeorgiadisH-Index: 4
Last. Waseem Qasim (GOSH: Great Ormond Street Hospital)H-Index: 28
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RNA polymerase III (Pol III) promoters express short non-coding RNAs and have been adopted for expression of microRNA, interference RNA, and CRISPR single guide RNA (sgRNA). Vectors incorporating H1 and U6 Pol III promoters are being applied for therapeutic genome editing, including multiplexed CRISPR/Cas9 effects. We report a nucleosome-depleted, minimal U6 promoter, which when embedded within lentiviral long terminal repeat (LTR) regions, supports high level transcriptional activity. Furthermo...
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#1C GeorgiadisH-Index: 4
#2Jane RasaiyaahH-Index: 13
Last. Waseem Qasim (GOSH: Great Ormond Street Hospital)H-Index: 28
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#1Roland PreeceH-Index: 2
#2C GeorgiadisH-Index: 4
Gene editing tools are being rapidly developed, accelerating many areas of cell and gene therapy research. Each successive gene editing technology promises increased efficacy, improved specificity, reduced manufacturing cost and design complexity; all of which are currently epitomised by the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas9) platform. Since its conceptualisation, CRISPR-based gene editing has been applied to existing methodolo...
1 CitationsSource
#1Jane RasaiyaahH-Index: 1
#2C GeorgiadisH-Index: 4
Last. Waseem QasimH-Index: 28
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4 CitationsSource
#1C GeorgiadisH-Index: 4
#2Roland PreeceH-Index: 2
Last. Waseem Qasim (GOSH: Great Ormond Street Hospital)H-Index: 28
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Gene editing can be used to overcome allo-recognition, which otherwise limits allogeneic T cell therapies. Initial proof-of-concept applications have included generation of such "universal" T cells expressing chimeric antigen receptors (CARs) against CD19 target antigens combined with transient expression of DNA-targeting nucleases to disrupt the T cell receptor alpha constant chain (TRAC). Although relatively efficient, transgene expression and editing effects were unlinked, yields variable, an...
16 CitationsSource
#1C GeorgiadisH-Index: 4
#2Roland PreeceH-Index: 2
Last. Waseem QasimH-Index: 28
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