Rafael A. Homem
Rothamsted Research
Publications 4
#1Bartlomiej J. Troczka (University of Exeter)H-Index: 5
#2Rafael A. Homem (Rothamsted Research)H-Index: 2
Last.T.G. Emyr Davies (Rothamsted Research)H-Index: 15
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Abstract Recent work has shown that two bumblebee (Bombus terrestris) cytochrome P450s of the CYP9Q subfamily, CYP9Q4 and CYP9Q5, are important biochemical determinants of sensitivity to neonicotinoid insecticides. Here, we report the characterisation of a third P450 gene CYP9Q6, previously mis-annotated in the genome of B. terrestris, encoding an enzyme that metabolises the N-cyanoamidine neonicotinoids thiacloprid and acetamiprid with high efficiency. The genomic location and complete ORF of C...
#1Bartlomiej J. Troczka (Rothamsted Research)H-Index: 5
#2Ewan Richardson (Rothamsted Research)H-Index: 1
Last.T.G. Emyr Davies (Rothamsted Research)H-Index: 15
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Abstract Using publicly available genomic data, combined with RT-PCR validation, we explore structural genomic variation for two major ion channels across insect classes. We have manually curated ryanodine receptor (RyR) and inositol 1,4,5-trisphosphate receptor (IP3R) ORFs and their corresponding genomic structures from 26 different insects covering major insect orders. We found that, despite high protein identity for both RyRs (>75%) and IP3Rs (~67%), the overall complexity of the gene structu...
2 CitationsSource
In this short review, we highlight three functional genomic technologies that have recently been contributing to the understanding of the molecular mechanisms underpinning insecticide resistance: the GAL4/UAS system, a molecular tool used to express genes of interest in a spatiotemporal controlled manner; the RNAi system, which is used to knock-down gene expression; and the most recently developed gene editing tool, CRISPR/Cas9, which can be used to knock-out and knock-in sequences of interest.
3 CitationsSource
#1Cristina Manjon (Bayer)H-Index: 3
#2Bartlomiej J. Troczka (Rothamsted Research)H-Index: 5
Last.Ralf Nauen (Bayer)H-Index: 56
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Summary The impact of neonicotinoid insecticides on the health of bee pollinators is a topic of intensive research and considerable current debate [1]. As insecticides, certain neonicotinoids, i.e., N -nitroguanidine compounds such as imidacloprid and thiamethoxam, are as intrinsically toxic to bees as to the insect pests they target. However, this is not the case for all neonicotinoids, with honeybees orders of magnitude less sensitive to N -cyanoamidine compounds such as thiacloprid [2]. Altho...
37 CitationsSource