Laura Marchetti
Istituto Italiano di Tecnologia
Publications 30
#1Robert Yan ('KCL': King's College London)H-Index: 4
#2Havva Yalinca (UCL: University College London)H-Index: 2
Last.Annalisa Pastore (UNIPV: University of Pavia)H-Index: 58
view all 11 authors...
Summary Nerve growth factor (NGF) is an important neurotrophic factor involved in the regulation of cell differentiation and survival of target neurons. Expressed as a proNGF precursor, NGF is matured by furin-mediated protease cleavage. Increasing evidence suggests that NGF and proNGF have distinct functional roles. While the structure of mature NGF is available, little is known about that of the pro-domain because of its dynamical structural features. We exploited an ad hoc hybrid strategy bas...
2 CitationsSource
#1William Durso (IIT: Istituto Italiano di Tecnologia)H-Index: 2
#2Francesca D'Autilia (IIT: Istituto Italiano di Tecnologia)H-Index: 6
Last.Francesco Cardarelli (Nest: Nest Labs)H-Index: 25
view all 5 authors...
Abstract Lysosomes are not merely degradative organelles but play a central role in nutrient sensing, metabolism and cell-growth regulation. Our ability to study their function in living cells strictly relies on the use of lysosome-specific fluorescent probes tailored to optical microscopy applications. Still, no report thus far quantitatively analyzed the effect of labeling strategies/procedures on lysosome properties in live cells. We tackle this issue by a recently developed spatiotemporal fl...
1 CitationsSource
#1Domenica Convertino (IIT: Istituto Italiano di Tecnologia)H-Index: 8
#2Stefano Luin (Nest: Nest Labs)H-Index: 15
Last.Camilla Coletti (IIT: Istituto Italiano di Tecnologia)H-Index: 19
view all 4 authors...
Graphene displays properties that make it appealing for neuroregenerative medicine, yet its interaction with peripheral neurons has been scarcely investigated. Here, we culture on graphene two established models for peripheral neurons: PC12 cells and DRG primary neurons. We perform a nano-resolved analysis of polymeric coatings on graphene and combine optical microscopy and viability assays to assess the material cytocompatibility and influence on differentiation. We find that differentiated PC1...
7 CitationsSource
#1Francesco GobboH-Index: 4
#2Fulvio Bonsignore (Nest: Nest Labs)H-Index: 2
Last.Laura Marchetti (IIT: Istituto Italiano di Tecnologia)H-Index: 12
view all 5 authors...
2 CitationsSource
#1Francesco Gobbo (Nest: Nest Labs)H-Index: 4
#2Laura Marchetti (IIT: Istituto Italiano di Tecnologia)H-Index: 12
Last.Antonino CattaneoH-Index: 34
view all 12 authors...
Increasing evidence points to the importance of dendritic spines in the formation and allocation of memories, and alterations of spine number and physiology are associated to memory and cognitive disorders. Modifications of the activity of subsets of synapses are believed to be crucial for memory establishment. However, the development of a method to directly test this hypothesis, by selectively controlling the activity of potentiated spines, is currently lagging. Here we introduce a hybrid RNA/...
5 CitationsSource
Neurotrophins are growth factors of fundamental importance for the development, survival and maintenance of different neuronal and non-neuronal populations. Over the years, the use of labelled neurotrophins has helped in the study of their biological functions, leading to a better understanding of the processes that regulate their transport, traffic and signalling. However, the diverse and heterogeneous neurotrophin labelling strategies adopted so far have often led to poorly reproducible protoc...
2 CitationsSource
#1Valentina Cappello (IIT: Istituto Italiano di Tecnologia)H-Index: 14
#2Laura Marchetti (IIT: Istituto Italiano di Tecnologia)H-Index: 12
Last.Mauro Gemmi (IIT: Istituto Italiano di Tecnologia)H-Index: 24
view all 8 authors...
Krabbe disease (KD) is a neurodegenerative disorder caused by the lack of β- galactosylceramidase enzymatic activity and by widespread accumulation of the cytotoxic galactosyl-sphingosine in neuronal, myelinating and endothelial cells. Despite the wide use of Twitcher mice as experimental model for KD, the ultrastructure of this model is partial and mainly addressing peripheral nerves. More details are requested to elucidate the basis of the motor defects, which are the first to appear during KD...
1,733 CitationsSource
#1Giuseppe Fiume (IIT: Istituto Italiano di Tecnologia)H-Index: 2
#2Carmine Di Rienzo (IIT: Istituto Italiano di Tecnologia)H-Index: 8
Last.Francesco Cardarelli (IIT: Istituto Italiano di Tecnologia)H-Index: 25
view all 6 authors...
Abstract Here we address the process of lipofection by quantifying the expression of a genetically-encoded fluorescent reporter at the single-cell level, and in real-time, by confocal imaging in live cells. The Lipofectamine gold-standard formulation is compared to the alternative promising DC-Chol/DOPE formulation. In both cases, we report that only dividing cells are able to produce a detectable amount of the fluorescent reporter protein. Notably, by measuring fluorescence over time in each pa...
3 CitationsSource
#1Teresa De NadaiH-Index: 3
#2Laura MarchettiH-Index: 12
Last.Antonino CattaneoH-Index: 34
view all 13 authors...
The classical view of nerve growth factor (NGF) action in the nervous system is linked to its retrograde axonal transport. However, almost nothing is known on the trafficking properties of its unprocessed precursor proNGF, characterized by different and generally opposite biological functions with respect to its mature counterpart. Here we developed a strategy to fluorolabel both purified precursor and mature neurotrophins (NTs) with a controlled stoichiometry and insertion site. Using a single ...
9 CitationsSource
#1Teresa De NadaiH-Index: 3
#2Laura MarchettiH-Index: 12
Last.Antonino CattaneoH-Index: 34
view all 13 authors...
Corrigendum: Precursor and mature NGF live tracking: one versus many at a time in the axons