Alexander McPherson
University of Alabama in Huntsville
CrystallizationSatellite tobacco mosaic virusCrystalCrystallographyBiology
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Publications 6
2 CitationsSource
#1Yuri G. Kuznetsov (UAH: University of Alabama in Huntsville)H-Index: 1
#2Jeffrey J. Dowell (UAH: University of Alabama in Huntsville)H-Index: 1
Last. Alexander McPherson (UAH: University of Alabama in Huntsville)H-Index: 2
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Agarose gel electrophoresis, circular dichroism and differential scanning calorimetry showed that single-stranded RNA from satellite tobacco mosaic virus transforms from a conformationally ‘closed state’ at 4 � C to a more conformationally ‘open state’ at 65 � C. The transition is reversible and shows no hysteresis. Atomic force microscopy (AFM) allowed visualization of the two states and indicated that the conformationally ‘closed state’ probably corresponds to the native encapsidated conformat...
13 CitationsSource
#1Ana P. Barba-de la Rosa (Instituto Potosino de Investigación Científica y Tecnológica)
#2Joseph D. NgH-Index: 7
Last. Alexander McPhersonH-Index: 2
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Satellite tobacco mosaic virus (STMV) has been detected in natural mixed infections in tobacco plants and in several horticultural crops co-infected with tobacco mosaic virus (TMV). TMV is a well characterized virus, but STMV was not discovered until 1986, and in 1993, a unique RNA arrangement inside the capsid was reported, which was of special interest as it extended the knowledge of the structural and functional characteristics of the STMV. In this study STMV was purified from infected tobacc...
#1Joseph D. NgH-Index: 7
#2Bernard LorberH-Index: 28
Last. Alexander McPhersonH-Index: 2
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The protein thaumatin was studied as a model macromolecule for crystallization in microgravity-environment experiments conducted on two US Space Shuttle missions (USML-2 and LMS). In this investigation, we have evaluated and compared the quality of space- and earth-grown thaumatin crystals using X-ray diffraction analyses, and characterized them according to crystal size, diffraction resolution limit and mosaicity. Two different approaches for growing thaumatin crystals in the microgravity envir...
36 CitationsSource
Xylanase from the bacterial plant pathogen Erwinia chrysanthemi (E.C., expressed in E. coli, has been crystallized for X-ray diffraction analysis both in the presence and the absence of its polymeric substrate 4-O-methyl glucuronoxylan. In all cases it was found that the quality, time of appearance, and reproducibility of both the native and complex crystals were significantly enhanced by heating of the protein to 323K prior to dispensing the crystallization trials. Crystals of the nati...
1 CitationsSource