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Feng Li
Sun Yat-sen University
Telomerase reverse transcriptaseTelomereBiologyTelomeraseCancer cell
12Publications
6H-index
141Citations
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Publications 11
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#1Feng Li (SYSU: Sun Yat-sen University)H-Index: 6
#2Yuanlong Ge (SYSU: Sun Yat-sen University)H-Index: 6
Last. Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 46
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Pluripotent stem cells (PSCs) such as embryonic stem cells (ESCs), ESCs derived by somatic cell nuclear transfer (ntESCs), and induced pluripotent stem cells (iPSCs) have unlimited capacity for self-renewal and pluripotency and can give rise to all types of somatic cells. In order to maintain their self-renewal and pluripotency, PSCs need to preserve their telomere length and homeostasis. In recent years, increasing studies have shown that telomere reprogramming is essential for stem cell plurip...
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#1Yuanlong Ge (SYSU: Sun Yat-sen University)H-Index: 6
#2Shu Wu (SYSU: Sun Yat-sen University)H-Index: 5
Last. Yong Zhao (SYSU: Sun Yat-sen University)H-Index: 34
view all 9 authors...
: In the original publication the labels in figure 4C and D are incorrectly published. The correct labels for figure 4C and D is provided in this correction.
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#1Zhenhua Luo (SYSU: Sun Yat-sen University)H-Index: 6
#2Weixu Wang (SCAU: South China Agricultural University)H-Index: 1
Last. Yuanyan Xiong (SYSU: Sun Yat-sen University)H-Index: 7
view all 8 authors...
Cancer cells become immortalized through telomere maintenance mechanisms, such as telomerase reverse transcriptase (TERT) activation. In addition to maintaining telomere length, TERT activates manifold cell survival signaling pathways. However, telomerase-associated gene signatures in cancer remain elusive. We performed a systematic analysis of TERT high (TERThigh) and low (TERTlow) cancers using multidimensional data from The Cancer Genome Atlas (TCGA). Multidimensional data were analyzed by pr...
3 CitationsSource
#1Yuanlong Ge (SYSU: Sun Yat-sen University)H-Index: 6
#2Shu Wu (SYSU: Sun Yat-sen University)H-Index: 5
Last. Yong Zhao (SYSU: Sun Yat-sen University)H-Index: 34
view all 9 authors...
While the majority of all human cancers counteract telomere shortening by expressing telomerase, ~15% of all cancers maintain telomere length by a telomerase-independent mechanism known as alternative lengthening of telomeres (ALT). Here, we show that high load of intrinsic DNA damage is present in ALT cancer cells, leading to apoptosis stress by activating p53-independent, but JNK/c-Myc-dependent apoptotic pathway. Notably, ALT cells expressing wild-type p53 show much lower apoptosis than p53-d...
6 CitationsSource
#1Feng Li (SYSU: Sun Yat-sen University)H-Index: 6
#2Hyeung Kim (BCM: Baylor College of Medicine)H-Index: 12
Last. Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 46
view all 15 authors...
Summary Telomeres and telomere-binding proteins form complex secondary nucleoprotein structures that are critical for genome integrity but can present serious challenges during telomere DNA replication. It remains unclear how telomere replication stress is resolved during S phase. Here, we show that the BUB3-BUB1 complex, a component in spindle assembly checkpoint, binds to telomeres during S phase and promotes telomere DNA replication. Loss of the BUB3-BUB1 complex results in telomere replicati...
17 CitationsSource
#1Hyeung Kim (BCM: Baylor College of Medicine)H-Index: 12
#2Feng Li (SYSU: Sun Yat-sen University)H-Index: 6
Last. Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 46
view all 10 authors...
CRISPR/Cas9 technology enables efficient loss-of-function analysis of human genes using somatic cells. Studies of essential genes, however, require conditional knockout (KO) cells. Here, we describe the generation of inducible CRISPR KO human cell lines for the subunits of the telosome/shelterin complex, TRF1, TRF2, RAP1, TIN2, TPP1 and POT1, which directly interact with telomeres or can bind to telomeres through association with other subunits. Homozygous inactivation of several subunits is let...
21 CitationsSource
#1Tianpeng Zhang (SYSU: Sun Yat-sen University)H-Index: 4
#1Tianpeng Zhang (SYSU: Sun Yat-sen University)H-Index: 2
Last. Yong Zhao (SYSU: Sun Yat-sen University)H-Index: 34
view all 13 authors...
Abstract Repetitive DNA is prone to replication fork stalling, which can lead to genome instability. Here, we find that replication fork stalling at telomeres leads to the formation of t ‐circle‐tails, a new extrachromosomal structure that consists of circular telomeric DNA with a single‐stranded tail. Structurally, the t ‐circle‐tail resembles cyclized leading or lagging replication intermediates that are excised from the genome by topoisomerase II‐mediated cleavage. We also show that the DNA d...
13 CitationsSource
#1Yuanlong Ge (SYSU: Sun Yat-sen University)H-Index: 6
#2Shu Wu (SYSU: Sun Yat-sen University)H-Index: 5
Last. Yong Zhao (SYSU: Sun Yat-sen University)H-Index: 34
view all 10 authors...
The majority of tumor cells overcome proliferative limit by expressing telomerase. Whether or not telomerase preferentially extends the shortest telomeres is still under debate. When human cancer cells are cultured at neutral pH, telomerase extends telomeres in telomere length-independent manner. However, the microenvironment of tumor is slightly acidic, and it is not yet known how this influences telomerase action. Here, we examine telomere length homeostasis in tumor cells cultured at pHe 6.8....
6 CitationsSource
#1Yang Hu (SYSU: Sun Yat-sen University)H-Index: 3
#2Guang Shi (SYSU: Sun Yat-sen University)H-Index: 7
Last. Junjiu Huang (SYSU: Sun Yat-sen University)H-Index: 22
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Activation of telomerase or alternative lengthening of telomeres (ALT) is necessary for tumours to escape from dysfunctional telomere-mediated senescence. Anti-telomerase drugs might be effective in suppressing tumour growth in approximately 85–90% of telomerase-positive cancer cells. However, there are still chances for these cells to bypass drug treatment after switching to the ALT mechanism to maintain their telomere integrity. But the mechanism underlying this switch is unknown. In this stud...
35 CitationsSource
#1Youwei Zhang (SYSU: Sun Yat-sen University)H-Index: 2
#2Yangxiu Wu (SYSU: Sun Yat-sen University)H-Index: 2
Last. Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 46
view all 13 authors...
The telomerase is responsible for adding telomeric repeats to chromosomal ends and consists of the reverse transcriptase TERT and the RNA subunit TERC. The expression and activity of the telomerase are tightly regulated, and aberrant activation of the telomerase has been observed in >85% of human cancers. To better understand telomerase regulation, we performed immunoprecipitations coupled with mass spectrometry (IP-MS) and identified cold inducible RNA-binding protein (CIRP or hnRNP A18) as a t...
20 CitationsSource
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