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Ming Cheng
Washington University in St. Louis
CatalysisProtein footprintingOrganic chemistryChemistryFootprinting
17Publications
7H-index
112Citations
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Publications 14
Newest
#1Ming Cheng (WashU: Washington University in St. Louis)H-Index: 7
#2Awuri Asuru (Case Western Reserve University)H-Index: 2
Last. Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 6 authors...
Synchrotron radiolysis generates hydroxyl radicals (*OH) that are successful footprinting reagents. Here, we describe a new reagent for the synchrotron platform, the trifluoromethyl radical (*CF3). The radical is produced by *OH displacement of *CF3 from sodium triflinate (Langlois reagent). Upon X-ray beam exposure, the reagent labels proteins extensively without any additional chemicals on the millisecond or shorter timescale. The *CF3 is comparably reactive to *OH and produces footprinting in...
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#1Ming ChengH-Index: 7
#2Chunyang GuoH-Index: 4
Last. Michael L. GrossH-Index: 75
view all 3 authors...
: Chemical approaches, in combination with mass- spectrometry analysis, have emerged as powerful tools to enable a broad range of biological applications. This approach is called structural proteomics. The key to success is a biocompatible reagent that modifies a protein without affecting its high-order structure. Fluorine, well-known to exert profound effects on the physical and chemical properties of reagents, should have an impact on structural proteomics. Here, we describe several fluorine-c...
1 CitationsSource
#1Ming Cheng (WashU: Washington University in St. Louis)H-Index: 7
#2Chunyang Guo (WashU: Washington University in St. Louis)H-Index: 4
Last. Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 3 authors...
1 CitationsSource
#1Chunyang Guo (WashU: Washington University in St. Louis)H-Index: 4
#2Lindsey K. Steinberg (WashU: Washington University in St. Louis)H-Index: 2
Last. Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 6 authors...
Both host and pathogen competitively manipulate coordination environments during bacterial infections. Human cells release the innate immune protein siderocalin (Scn, also known as lipocalin-2/Lcn2, neutrophil gelatinase-associated lipocalin/NGAL) that can inhibit bacterial growth by sequestering iron in a ferric complex with enterobactin (Ent), the ubiquitous Escherichia coli siderophore. Pathogenic E. coli use the virulence-associated esterase IroE to linearize the Ent cyclic trilactone to lin...
2 CitationsSource
#1Katherine Basore (WashU: Washington University in St. Louis)H-Index: 6
#1K.M. Basore (WashU: Washington University in St. Louis)H-Index: 1
Last. Daved H. Fremont (WashU: Washington University in St. Louis)H-Index: 66
view all 11 authors...
Summary Mxra8 is a receptor for multiple arthritogenic alphaviruses that cause debilitating acute and chronic musculoskeletal disease in humans. Herein, we present a 2.2 A resolution X-ray crystal structure of Mxra8 and 4 to 5 A resolution cryo-electron microscopy reconstructions of Mxra8 bound to chikungunya (CHIKV) virus-like particles and infectious virus. The Mxra8 ectodomain contains two strand-swapped Ig-like domains oriented in a unique disulfide-linked head-to-head arrangement. Mxra8 bin...
8 CitationsSource
#1Haijun Liu (WashU: Washington University in St. Louis)H-Index: 28
#1Haijun Liu (WashU: Washington University in St. Louis)H-Index: 17
Last. Robert E. Blankenship (WashU: Washington University in St. Louis)H-Index: 63
view all 10 authors...
ABSTRACT Cyanobacterial phycobilisomes (PBSs) are photosynthetic antenna complexes that harvest light energy and supply it to two reaction centers (RCs) where photochemistry starts. PBSs can be classified into two types, depending on the presence of allophycocyanin (APC): CpcG-PBS and CpcL-PBS. Because the accurate protein composition of CpcL-PBS remains unclear, we describe here its isolation and characterization from the cyanobacterium Synechocystis sp. strain 6803. We found that ferredoxin-NA...
5 CitationsSource
#1Chunyang Guo (WashU: Washington University in St. Louis)H-Index: 4
#2Ming Cheng (WashU: Washington University in St. Louis)H-Index: 7
Last. Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 3 authors...
Metal ions, usually bound by various amino-acid side chains in proteins, play multiple roles in protein folding, conformational change, cellular communication, and catalysis. Ca(II) and Mg(II), abundant among biologically relevant cations, execute their cellular functions associated with the conformational change of bound proteins. They bind with proteins where carboxylic acid residues are dominant ligands. To develop mass spectrometry for mapping protein-binding sites, we implemented a new carb...
4 CitationsSource
#1Bojie Zhang (WashU: Washington University in St. Louis)H-Index: 5
#2Ming Cheng (WashU: Washington University in St. Louis)H-Index: 7
Last. Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 4 authors...
Abstract Fast photochemical oxidation of proteins (FPOP) is a footprinting technique used in mass spectrometry-based structural proteomics. It has been applied to solve a variety of problems in different areas of biology. A FPOP platform requires a laser, optics, and sample flow path properly assembled to enable fast footprinting. Sample preparation, buffer conditions, and reagent concentrations are essential to obtain reasonable oxidations on proteins. FPOP samples can be analyzed by LC–MS meth...
5 CitationsSource
#1Ming Cheng (WashU: Washington University in St. Louis)H-Index: 7
#2Bojie Zhang (WashU: Washington University in St. Louis)H-Index: 5
Last. Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 4 authors...
Described is a novel, laser-initiated radical trifluoromethylation for protein footprinting and its broad residue coverage. .CF3 reacts with 18 of the 20 common amino acids, including Gly, Ala, Ser, Thr, Asp, and Glu, which are relatively silent with regard to .OH. This new approach to footprinting is a bridge between trifluoromethylation in materials and medicinal chemistry and structural biology and biotechnology. Its application to a membrane protein and to myoglobin show that the approach is...
16 CitationsSource
#1Jinghe Yuan (CAS: Chinese Academy of Sciences)H-Index: 8
#2Kangmin He (CAS: Chinese Academy of Sciences)H-Index: 12
Last. Xiaohong Fang (CAS: Chinese Academy of Sciences)H-Index: 35
view all 5 authors...
The step analysis of single-molecule photobleaching data offers a new approach for studying protein stoichiometry under physiological conditions. As such, it is important to develop suitable algorithms that can accurately extract the step events from the noisy single-molecule data. Herein, we report a HMM method that combines maximum-likelihood clustering for initializing the emission-probability distribution of the HMMs with an extended silhouette clustering criterion for estimating the state n...
8 CitationsSource
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