Match!
Ming Cheng
Washington University in St. Louis
11Publications
6H-index
82Citations
Publications 11
Newest
#1Ming ChengH-Index: 6
#2Chunyang GuoH-Index: 1
Last.Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 3 authors...
Source
#1K.M. Basore (WashU: Washington University in St. Louis)H-Index: 1
#2Arthur S. Kim (WashU: Washington University in St. Louis)H-Index: 4
Last.Daved H. Fremont (WashU: Washington University in St. Louis)H-Index: 62
view all 11 authors...
Summary Mxra8 is a receptor for multiple arthritogenic alphaviruses that cause debilitating acute and chronic musculoskeletal disease in humans. Herein, we present a 2.2 A resolution X-ray crystal structure of Mxra8 and 4 to 5 A resolution cryo-electron microscopy reconstructions of Mxra8 bound to chikungunya (CHIKV) virus-like particles and infectious virus. The Mxra8 ectodomain contains two strand-swapped Ig-like domains oriented in a unique disulfide-linked head-to-head arrangement. Mxra8 bin...
2 CitationsSource
#1Haijun Liu (WashU: Washington University in St. Louis)H-Index: 17
#2Daniel A. Weisz (WashU: Washington University in St. Louis)H-Index: 7
Last.Robert E. Blankenship (WashU: Washington University in St. Louis)H-Index: 63
view all 10 authors...
ABSTRACT Cyanobacterial phycobilisomes (PBSs) are photosynthetic antenna complexes that harvest light energy and supply it to two reaction centers (RCs) where photochemistry starts. PBSs can be classified into two types, depending on the presence of allophycocyanin (APC): CpcG-PBS and CpcL-PBS. Because the accurate protein composition of CpcL-PBS remains unclear, we describe here its isolation and characterization from the cyanobacterium Synechocystis sp. strain 6803. We found that ferredoxin-NA...
1 CitationsSource
#1Chunyang Guo (WashU: Washington University in St. Louis)H-Index: 1
#2Ming Cheng (WashU: Washington University in St. Louis)H-Index: 6
Last.Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 3 authors...
Metal ions, usually bound by various amino-acid side chains in proteins, play multiple roles in protein folding, conformational change, cellular communication, and catalysis. Ca(II) and Mg(II), abundant among biologically relevant cations, execute their cellular functions associated with the conformational change of bound proteins. They bind with proteins where carboxylic acid residues are dominant ligands. To develop mass spectrometry for mapping protein-binding sites, we implemented a new carb...
1 CitationsSource
#1Bojie Zhang (WashU: Washington University in St. Louis)H-Index: 4
#2Ming Cheng (WashU: Washington University in St. Louis)H-Index: 6
Last.Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 4 authors...
Abstract Fast photochemical oxidation of proteins (FPOP) is a footprinting technique used in mass spectrometry-based structural proteomics. It has been applied to solve a variety of problems in different areas of biology. A FPOP platform requires a laser, optics, and sample flow path properly assembled to enable fast footprinting. Sample preparation, buffer conditions, and reagent concentrations are essential to obtain reasonable oxidations on proteins. FPOP samples can be analyzed by LC–MS meth...
5 CitationsSource
#1Ming Cheng (WashU: Washington University in St. Louis)H-Index: 6
#2Bojie Zhang (WashU: Washington University in St. Louis)H-Index: 4
Last.Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 4 authors...
Described is a novel, laser-initiated radical trifluoromethylation for protein footprinting and its broad residue coverage. .CF3 reacts with 18 of the 20 common amino acids, including Gly, Ala, Ser, Thr, Asp, and Glu, which are relatively silent with regard to .OH. This new approach to footprinting is a bridge between trifluoromethylation in materials and medicinal chemistry and structural biology and biotechnology. Its application to a membrane protein and to myoglobin show that the approach is...
11 CitationsSource
#1Jinghe Yuan (CAS: Chinese Academy of Sciences)H-Index: 8
#2Kangmin He (CAS: Chinese Academy of Sciences)H-Index: 11
Last.Xiaohong Fang (CAS: Chinese Academy of Sciences)H-Index: 39
view all 5 authors...
The step analysis of single-molecule photobleaching data offers a new approach for studying protein stoichiometry under physiological conditions. As such, it is important to develop suitable algorithms that can accurately extract the step events from the noisy single-molecule data. Herein, we report a HMM method that combines maximum-likelihood clustering for initializing the emission-probability distribution of the HMMs with an extended silhouette clustering criterion for estimating the state n...
8 CitationsSource
#1Feng Hu (CAS: Chinese Academy of Sciences)H-Index: 11
#2Jianwei Yan (CAS: Chinese Academy of Sciences)H-Index: 6
Last.Youhong Hu (CAS: Chinese Academy of Sciences)H-Index: 18
view all 4 authors...
3(2H)-Furanones are efficiently generated from 3-alkynyl oxireno[2,3-b]chromenones by an Au/DDQ-catalyzed domino reaction through a pathway composed of cyclization, CC cleavage, nucleophilic addition, oxidation, and nucleophilic addition. It was found that stoichiometric AuCl3 or catalytic Au with stoichiometric DDQ can oxidize the benzylic sp3 CH bond to facilitate nucleophilic addition.
7 CitationsSource
#1Ming Cheng (CAS: Chinese Academy of Sciences)H-Index: 6
#2Jianwei Yan (CAS: Chinese Academy of Sciences)H-Index: 6
Last.Youhong Hu (CAS: Chinese Academy of Sciences)H-Index: 18
view all 5 authors...
An efficient, palladium-catalyzed cascade reaction, which leads to formation of annulated xanthones, was devised. The process, which uses readily available 3-iodochromones, aryl iodides and norbornadiene as starting materials, takes place via a tandem Heck reaction/double C–H activation/retro-Diels–Alder pathway. The high chemoselectivity of the process is mechanistically unique and it serves as a new approach to achieve regioselective control of C–H activation in Pd/norbornene or norbornadiene ...
20 CitationsSource
#1Jianwei YanH-Index: 6
#2Ming ChengH-Index: 6
Last.Youhong HuH-Index: 18
view all 4 authors...
NH aldimines, generated in situ from the corresponding aldehydes by reaction with ammonium acetate, serve as nitrogen nucleophiles in reactions with 3-(1-alkynyl)chromones and 3-cyanochromones that generate functionalized azaxanthones. These processes take place under mild conditions that do not require dry solvents. The products of the reactions described represent new chemical entities. We believe that the newly developed cascade process will serve as a potent method for the synthesis of N-het...
18 CitationsSource
12