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Ning Yao
Shanghai Jiao Tong University
GeneGene knockinZygoteBiologyCell biology
3Publications
2H-index
44Citations
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Publications 3
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#1Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 5
#2Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 17 authors...
Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and four-cell embryos results in much higher (up to 13-fold) homozygotic nucleotide substitution efficiency as opposed to MII oocytes or zygotes. Furthermore, as a proof-of-principle study, a point mutation can be ...
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#1Xuan Yao (CAS: Chinese Academy of Sciences)H-Index: 8
#2Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 5
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 16 authors...
Summary The targeting efficiency of knockin sequences via homologous recombination (HR) is generally low. Here we describe a method we call Tild-CRISPR (targeted integration with linearized dsDNA-CRISPR), a targeting strategy in which a PCR-amplified or precisely enzyme-cut transgene donor with 800-bp homology arms is injected with Cas9 mRNA and single guide RNA into mouse zygotes. Compared with existing targeting strategies, this method achieved much higher knockin efficiency in mouse embryos, ...
19 CitationsSource
#1Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
#2Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 5
Last. Zi-Jiang Chen (SJTU: Shanghai Jiao Tong University)H-Index: 39
view all 12 authors...
25 CitationsSource
1