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Ning Yao
Shanghai Jiao Tong University
2Publications
2H-index
25Citations
Publications 2
Newest
#1Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 2
#2Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 3
Last.Ning Yao (SJTU: Shanghai Jiao Tong University)H-Index: 2
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Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and four-cell embryos results in much higher (up to 13-fold) homozygotic nucleotide substitution efficiency as opposed to MII oocytes or zygotes. Furthermore, as a proof-of-principle study, a point mutation can be ...
#1Xuan Yao (CAS: Chinese Academy of Sciences)H-Index: 7
#2Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 2
Last.Yun Sun (SJTU: Shanghai Jiao Tong University)H-Index: 9
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Summary The targeting efficiency of knockin sequences via homologous recombination (HR) is generally low. Here we describe a method we call Tild-CRISPR (targeted integration with linearized dsDNA-CRISPR), a targeting strategy in which a PCR-amplified or precisely enzyme-cut transgene donor with 800-bp homology arms is injected with Cas9 mRNA and single guide RNA into mouse zygotes. Compared with existing targeting strategies, this method achieved much higher knockin efficiency in mouse embryos, ...
#1Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 3
#2Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 2
Last.Weiping Li (SJTU: Shanghai Jiao Tong University)H-Index: 7
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