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Ning Yao
Shanghai Jiao Tong University
2Publications
2H-index
25Citations
Publications 2
Newest
Published on Dec 1, 2019in Genome Biology14.03
Meiling Zhang2
Estimated H-index: 2
(SJTU: Shanghai Jiao Tong University),
Changyang Zhou3
Estimated H-index: 3
(CAS: Chinese Academy of Sciences)
+ 14 AuthorsNing Yao2
Estimated H-index: 2
(SJTU: Shanghai Jiao Tong University)
Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and four-cell embryos results in much higher (up to 13-fold) homozygotic nucleotide substitution efficiency as opposed to MII oocytes or zygotes. Furthermore, as a proof-of-principle study, a point mutation can be ...
Published on May 1, 2018in Developmental Cell9.19
Xuan Yao7
Estimated H-index: 7
(CAS: Chinese Academy of Sciences),
Meiling Zhang2
Estimated H-index: 2
(SJTU: Shanghai Jiao Tong University)
+ 13 AuthorsYun Sun9
Estimated H-index: 9
(SJTU: Shanghai Jiao Tong University)
Summary The targeting efficiency of knockin sequences via homologous recombination (HR) is generally low. Here we describe a method we call Tild-CRISPR (targeted integration with linearized dsDNA-CRISPR), a targeting strategy in which a PCR-amplified or precisely enzyme-cut transgene donor with 800-bp homology arms is injected with Cas9 mRNA and single guide RNA into mouse zygotes. Compared with existing targeting strategies, this method achieved much higher knockin efficiency in mouse embryos, ...
Published on Oct 1, 2017in Protein & Cell7.58
Changyang Zhou3
Estimated H-index: 3
(CAS: Chinese Academy of Sciences),
Meiling Zhang2
Estimated H-index: 2
(SJTU: Shanghai Jiao Tong University)
+ 9 AuthorsWeiping Li7
Estimated H-index: 7
(SJTU: Shanghai Jiao Tong University)
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