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Ruy D. Chacón
University of São Paulo
4Publications
1H-index
4Citations
Publications 4
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#1Ruy D. Chacón (USP: University of São Paulo)H-Index: 1
#2Claudete S. Astolfi-Ferreira (USP: University of São Paulo)H-Index: 8
Last.Antonio José Piantino Ferreira (USP: University of São Paulo)H-Index: 24
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Abstract Infectious bronchitis (IB) is one of the avian diseases with the greatest impact on poultry farming worldwide. In Brazil, strain BR-I (GI-11) is the most prevalent in poultry flocks. The present study aimed to develop a seminested RT-PCR assay specific for the diagnosis of BR-I IBV in Brazilian samples, targeting subunit 1 of the S gene. The detection limit of this assay was 10 copies of the IBV genome. In this study, 62.24% of 572 organ pools from the 5 regions of Brazil tested positiv...
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#1Ruy D. Chacón (USP: University of São Paulo)H-Index: 1
#2Quézia Moura (USP: University of São Paulo)H-Index: 5
Last.Antonio José Piantino Ferreira (USP: University of São Paulo)H-Index: 24
view all 11 authors...
ABSTRACT This report describes the genome sequences of four Salmonella enterica subsp. enterica serovar Gallinarum (Salmonella Gallinarum) strains isolated in Colombia in 2017 from layer breeders of different ages. The layer breeder flocks were presenting with an elevated mortality with lesions typical of fowl typhoid (FT). These draft genome sequences revealed a highly conserved genome of Salmonella Gallinarum strains circulating in Colombia.
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Avian rotavirus A (ARtV-A) is a virus that affects young birds, causing acute diarrhea and economic losses in the poultry industry worldwide. The techniques used for the diagnosis of ARtV-A include electron microscopy, isolation in cell culture, and serology, as well as molecular techniques, such as the reverse transcription-polymerase chain reaction (RT-PCR). The objective of this work was to standardize a real-time RT-polymerase chain reaction (RT-qPCR) using SYBR Green chemistry for the rapid...
1 CitationsSource
#1Claudia Carranza (USP: University of São Paulo)H-Index: 3
#2Claudete S. Astolfi-Ferreira (USP: University of São Paulo)H-Index: 8
Last.Antonio José Piantino Ferreira (USP: University of São Paulo)H-Index: 24
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1. Infectious bronchitis virus (IBV) variants in Brazil were isolated during 2010-2015 for epidemiological and molecular analysis to characterise the different variants and perform a bioinformatic analysis to compare with sequences of variants collected over the previous 40 years. 2. Of the 453 samples examined, 61.4% were positive for IBV and 75.9% of these were considered to have the BR-I genotype and were detected in birds of all ages distributed in all five Brazilian regions. 3. The ratio of...
3 CitationsSource
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