Matthew Flavel
La Trobe University
AxenicCaenorhabditis elegansToxicityBiologyCell biology
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Publications 11
Increasing evidence suggests that environmental stress, such as UV radiation, generates reactive oxygen and nitrogen species in skin cells, leading to histochemical changes including skin disorders and aging, hyper pigmentation, and increased formation of wrinkles. Besides the defensive system in skin composed of vitamins and intrinsic antioxidant enzymes, topical and skin conditioning products have been used commonly to eradicate or eliminate these skin ailments. Among various ingredients provi...
Abstract Evidence suggests that enhanced oxidative stress and mild, chronic inflammation is associated with the onset and progression of some neurological disorders. Hypothesis A polyphenol rich sugarcane extract may be active in protecting against this inflammation and neurological degeneration. Study design A series of cell culture strains and in vitro gene expression studies were used to understand the neuroprotective activity. Methods Extract was prepared according to a patented extraction p...
1 CitationsSource
#1Weiyang Chen (Qilu University of Technology)H-Index: 7
#2Weiwei Li (Qilu University of Technology)H-Index: 1
Last. Matthew Flavel (La Trobe University)H-Index: 1
view all 4 authors...
AbstractObjectives: Dysfunctional metabolism of carbohydrates is a fundamental component of many dietary-related disorders. It has been hypothesized that plant extracts containing high levels of antioxidants may have the ability to stabilize carbohydrate regulation. The aim of this study was to assess the effects of a polyphenol-rich sugarcane extract on cellular pathways related to carbohydrate metabolism.Methods: We evaluated the antioxidant activity of a polyphenol-rich sugarcane extract obta...
3 CitationsSource
#1Shan GaoH-Index: 2
#2Weiyang Chen (Qilu University of Technology)H-Index: 7
Last. Guojun Li (Capital Medical University)H-Index: 2
view all 13 authors...
Applying toxicity testing of chemicals in higher order organisms, such as mice or rats, is time-consuming and expensive, due to their long lifespan and maintenance issues. On the contrary, the nematode Caenorhabditis elegans (C. elegans) has advantages to make it an ideal choice for toxicity testing: a short lifespan, easy cultivation, and efficient reproduction. Here, we describe a protocol for the automatic phenotypic profiling of C. elegans in a 384-well plate. The nematode worms are cultured...
1 CitationsSource
#1Surafel M. Tegegne (La Trobe University)H-Index: 1
#2Markandeya Jois (La Trobe University)H-Index: 12
Last. Devin Benheim (La Trobe University)H-Index: 3
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Abstract Animal models of B12 deficiency have proven to be difficult due to storage of substantial amounts in the liver and the length of time required to maintain animals on a B12 deficient diet to induce deficiency. Caenorhabditis elegans (C. elegans), due to its short lifespan, has recently emerged as an alternate model to investigate vitamin B12 (B12) deficiency. However, when C. elegans are maintained on bacterial diet, five generations of B12 deficient diet is required before the worms sho...
#1Shan Gao (Capital Medical University)H-Index: 2
#2Weiyang Chen (Qilu University of Technology)H-Index: 7
Last. Guojun Li (Capital Medical University)H-Index: 2
view all 10 authors...
Background Traditional toxicological studies have relied heavily on various animal models to understand the effect of various compounds in a biological context. Considering the great cost, complexity and time involved in experiments using higher order organisms. Researchers have been exploring alternative models that avoid these disadvantages. One example of such a model is the nematode Caenorhabditis elegans. There are some advantages of C. elegans, such as small size, short life cycle, well de...
1 CitationsSource
#1Matthew Flavel (La Trobe University)H-Index: 1
#2Adam Mechler (La Trobe University)H-Index: 25
Last. Markandeya Jois (La Trobe University)H-Index: 12
view all 13 authors...
Caenorhabditis elegans are typically cultured in a monoxenic medium consisting of live bacteria. However, this introduces a secondary organism to experiments, and restricts the manipulation of the nutritional environment. Due to the intricate link between genes and environment, greater control and understanding of nutritional factors are required to push the C. elegans field into new areas. For decades, attempts to develop a chemically defined, axenic medium as an alternative for culturing C. el...
8 CitationsSource
#1Weiyang ChenH-Index: 7
#2Bo LiaoH-Index: 1
Last. Bo XianH-Index: 7
view all 8 authors...
: Image segmentation is a key process in analyzing biological images. However, it is difficult to detect the differences between foreground and background when the image is unevenly illuminated. The unambiguous segmenting of multi-well plate microscopy images with various uneven illuminations is a challenging problem. Currently, no publicly available method adequately solves these various problems in bright-field multi-well plate images. Here, we propose a new method based on contrast values whi...
1 CitationsSource
#1A. Yen (La Trobe University)H-Index: 1
#2Matthew Flavel (La Trobe University)H-Index: 1
Last. Boyd R. WrightH-Index: 8
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The bush coconut is used as a source of food by several Australian Aboriginal communities. It is actually a scaleinsect gall. Originally all bush coconut insects were given the same species name, but now there are at least three species in Australia. The bloodwood trees at Kiwirrkurra (Western Australia), Corymbia opaca, had bush coconuts built by the scale insect Cystococcus pomiformis. The use of the coconut is described by some Aboriginal women from Kiwirrkurra. The nutritional value of the b...
1 CitationsSource