Philip R Baldwin
Salk Institute for Biological Studies
Uniform distribution (continuous)Normalization (statistics)Fourier shell correlationBiologySampling (statistics)
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Publications 4
#1Philip R Baldwin (Salk Institute for Biological Studies)H-Index: 3
#2Dmitry Lyumkis (Salk Institute for Biological Studies)H-Index: 18
Abstract Virtually all single-particle cryo-EM experiments currently suffer from specimen adherence to the air-water interface, leading to a non-uniform distribution in the set of projection views. Whereas it is well accepted that uniform projection distributions can lead to high-resolution reconstructions, non-uniform (anisotropic) distributions can negatively affect map quality, elongate structural features, and in some cases, prohibit interpretation altogether. Although some consequences of n...
3 CitationsSource
#1Philip R BaldwinH-Index: 3
#2Yong Zi Tan (Columbia University)H-Index: 9
Last. Bridget Carragher (Columbia University)H-Index: 50
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The scope and complexity of cryogenic electron microscopy (cryoEM) data has greatly increased, and will continue to do so, due to recent and ongoing technical breakthroughs that have led to much improved resolutions for macromolecular structures solved using this method. This big data explosion includes single particle data as well as tomographic tilt series, both generally acquired as direct detector movies of ∼10–100 frames per image or per tilt-series. We provide a brief survey of the develop...
12 CitationsSource
#1Dmitry LyumkisH-Index: 18
#2Yong Zi TanH-Index: 9
Last. Philip R BaldwinH-Index: 3
view all 3 authors...
3 CitationsSource
#1Yong Zi TanH-Index: 9
#2Philip R BaldwinH-Index: 3
Last. Dmitry LyumkisH-Index: 18
view all 7 authors...
The preferred specimen orientation problem limits accuracy and resolution in structure determination by cryo-EM. Collecting data at defined sample tilts yielded near-atomic-resolution structures for the influenza hemagglutinin trimer and ribosomal biogenesis intermediates.
97 CitationsSource