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Ivan Yu. Gushchin
Moscow Institute of Physics and Technology
45Publications
10H-index
323Citations
Publications 45
Newest
Phosphatidylserine (PS) is a negatively charged lipid type commonly found in eukaryotic membranes, where it interacts with proteins via nonspecific electrostatic interactions as well as via specific binding. Moreover, in the presence of calcium ions, PS lipids can induce membrane fusion and phase separation. Molecular details of these phenomena remain poorly understood, partly because accurate models to interpret the experimental data have not been available. Here we gather a set of previously p...
#1Kirill KovalevH-Index: 5
#2Dmytro Volkov (Forschungszentrum Jülich)
Last.Alexander Popov (European Synchrotron Radiation Facility)H-Index: 18
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Rhodopsins are the most abundant light-harvesting proteins. A new family of rhodopsins, heliorhodopsins (HeRs), was recently discovered. In opposite to the known rhodopsins their N-termini face the cytoplasm. HeRs structure and function remain unknown. We present structures of two HeR-48C12 states at 1.5 A showing its remarkable difference from all known rhodopsins. Its internal extracellular part is completely hydrophobic, while the cytoplasmic part comprises a cavity (‘active site’), surrounde...
#1Vera V. Nazarenko (MIPT: Moscow Institute of Physics and Technology)
#2V.V. Nazarenko (MIPT: Moscow Institute of Physics and Technology)
Last.A. V. RogachevH-Index: 6
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Light-Oxygen-Voltage (LOV) domains are conserved parts of photoreceptors in plants, bacteria and fungi that bind flavins as chromophores and detect blue light. In the past, LOV domain variants have been developed as fluorescent reporter proteins (called flavin-based fluorescent proteins; FbFPs), which due to their ability to fluoresce under anaerobic conditions, fast folding kinetics and a small size of ∼12–16 kDa are a promising reporter system for quantitative real-time analysis of biological ...
#1Kirill KovalevH-Index: 5
#2Vitaly Polovinkin (CNRS: Centre national de la recherche scientifique)H-Index: 7
Last.Dmitry Bratanov (CNRS: Centre national de la recherche scientifique)H-Index: 1
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Rhodopsins are the most universal biological light-energy transducers and abundant phototrophic mechanisms that evolved on Earth and have a remarkable diversity and potential for biotechnological applications. Recently, the first sodium-pumping rhodopsin KR2 from Krokinobacter eikastus was discovered and characterized. However, the existing structures of KR2 are contradictory, and the mechanism of Na + pumping is not yet understood. Here, we present a structure of the cationic (non H + ) light-d...
#1Ivan Maslov (MIPT: Moscow Institute of Physics and Technology)H-Index: 1
#2Andrey O. Bogorodskiy (MIPT: Moscow Institute of Physics and Technology)H-Index: 2
Last.Valentin I. Gordeliy (MIPT: Moscow Institute of Physics and Technology)H-Index: 21
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Research on halophilic microorganisms is important due to their relation to fundamental questions of survival of living organisms in a hostile environment. Here we introduce a novel method to stain halophiles with MitoTracker fluorescent dyes in their growth medium. The method is based on membrane-potential sensitive dyes, which were originally used to label mitochondria in eukaryotic cells. We demonstrate that these fluorescent dyes provide high staining efficiency and are beneficial for multi-...
#1Pavel Buslaev (MIPT: Moscow Institute of Physics and Technology)H-Index: 3
#2Ivan Yu. Gushchin (MIPT: Moscow Institute of Physics and Technology)H-Index: 10
Molecular dynamics simulations are used extensively to study the processes on biological membranes. The simulations can be conducted at different levels of resolution: all atom (AA), where all atomistic details are provided; united atom (UA), where hydrogen atoms are treated inseparably of corresponding heavy atoms; and coarse grained (CG), where atoms are grouped into larger particles. Here, we study the behavior of model bilayers consisting of saturated and unsaturated lipids DOPC, SOPC, OSPC ...
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