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Shuji Sakamoto
Kyoto University
GeneMolecular biologyHistoneChromatinBiology
3Publications
3H-index
115Citations
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Publications 3
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#1Shuji Sakamoto (Kyoto University)H-Index: 3
#2Kousho Wakae (Kyoto University)H-Index: 2
Last. Yasutoshi AgataH-Index: 19
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V(D)J recombination of Ig and TCR genes is strictly regulated in a lineage- and stage-specific manner by the accessibility of target gene chromatin to the recombinases RAG1 and RAG2. It has been shown that enforced expression of the basic helix–loop–helix protein, E2A, together with RAG1/2 in a nonlymphoid cell line BOSC23 can induce V(D)J recombination in endogenous Igκ and TCR loci by increasing chromatin accessibility of target gene segments. In this study, we demonstrate that ectopically exp...
22 CitationsSource
#1Masatoshi NozakiH-Index: 12
#2Kousho Wakae (Kyoto University)H-Index: 2
Last. Yasutoshi Agata (Kyoto University)H-Index: 19
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V(D)J recombination of Ig and TCR genes is strictly regulated by the accessibility of target gene chromatin in a lineage- and stage-specific manner. In the mouse TCRg locus, rearrangement of the Vg2 gene predominates over Vg3 rearrangement in the adult thymus. This preferential rearrangement is likely due to the differential accessibility of the individual Vg genes, because the levels of germ line transcription and histone acetylation of the Vg genes are well correlated with the rearrangement fr...
4 CitationsSource
#1Yasutoshi Agata (Kyoto University)H-Index: 19
#2Nobuyuki Tamaki (Kyoto University)H-Index: 12
Last. Cornelis Murre (UCSD: University of California, San Diego)H-Index: 73
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Allelic exclusion of antigen-receptor genes is ensured primarily by monoallelic locus activation upon rearrangement and subsequently by feedback inhibition of continued rearrangement. Here, we demonstrated that the basic helix-loop-helix protein, E47, promoted T cell receptor β (TCRβ) gene rearrangement by directly binding to target gene segments to increase chromatin accessibility in a dosage-sensitive manner. Feedback signaling abrogated E47 binding, leading to a decline in accessibility. Conv...
89 CitationsSource
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