Yoko Kurotaki
Central Institute for Experimental Animals
Molecular biologyEmbryoMarmosetSpermatidBiology
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Publications 5
#1Narumi OgonukiH-Index: 42
#2Yukiko Abe (UTokyo: University of Tokyo)H-Index: 1
Last. Atsuo OguraH-Index: 60
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: The common marmoset is a small nonhuman primate in which the application of transgenesis and genetic knockout techniques allows the generation of gene-modified models of human diseases. However, its longer generation time than that of rodents is a major obstacle to the widespread use of gene-modified marmosets for biomedical research. In this study, we examined the feasibility of shortening the generation time by using prepubertal marmoset males as gamete donors. We collected late round stage ...
#1Narumi OgonukiH-Index: 42
#2Hiroki InoueH-Index: 15
Last. Atsuo Ogura (UTokyo: University of Tokyo)H-Index: 60
view all 9 authors...
: The common marmoset (Callithrix jacchus) represents a promising nonhuman primate model for the study of human diseases because of its small size, ease of handling, and availability of gene-modified animals. Here, we aimed to devise reproductive technology for marmoset spermatid injection using immature males for a possible rapid generational turnover. Spermatids at each step could be identified easily by their morphology under differential interference microscopy: thus, early round spermatids ...
2 CitationsSource
#1Akiko Uematsu (Central Institute for Experimental Animals)H-Index: 1
#2Jun-ichi Hata (Central Institute for Experimental Animals)H-Index: 43
Last. Hideyuki Okano (Keio: Keio University)H-Index: 112
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Abstract Brain development involves spatiotemporally complex microstructural changes. A number of neuropsychiatric disorders are linked to the neural processes of development and aging. Thus, it is important to understanding the typical developmental patterns of various brain structures, which will help to define critical periods of vulnerability for neural maturation, as well as anatomical mechanisms of brain structure-related neuropathology. In this study, we used magnetic resonance imaging to...
5 CitationsSource
#1Yoko Kurotaki (Central Institute for Experimental Animals)H-Index: 2
#2Erika Sasaki (Central Institute for Experimental Animals)H-Index: 21
Abstract: The common marmoset (Callithrix jacchus) is commonly used as a subject model animal in experimental research. The species has several advantages compared with other laboratory primates and we succeeded in creating a transgenic (Tg) marmoset with germline transmission of the transgene, the first time in a nonhuman primate. We have been attempting to further improve marmoset reproductive technology, which is more similar to that of humans than rodent experimental animals, such as mice. W...
2 CitationsSource
#1Yoko Kurotaki (University of Tsukuba)H-Index: 2
#2Yuki HatanakaH-Index: 9
Last. Atsuo Ogura (UTokyo: University of Tokyo)H-Index: 60
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STUDY QUESTION: Is the poor development of embryos generated from round spermatid injection (ROSI) in humans and animals associated with abnormal active DNA demethylation? SUMMARY ANSWER: A significant proportion of ROSI-derived embryos failed to undergo active DNA demethylation. WHAT IS KNOWN ALREADY: Active DNA demethylation is initiated by the conversion of 5-methylcytosine (5mC) to 5-hydroxycytosine (5hmC) by the Tet3 enzyme. Active demethylation proceeds in a more pronounced manner in the m...
12 CitationsSource