Kyoji Hagiwara
Mie University
Publications 8
#1Kyoji HagiwaraH-Index: 5
#2Hideki IshiiH-Index: 30
Last.Yoko AidaH-Index: 29
view all 13 authors...
The emergence of multidrug-resistant viruses compromises the efficacy of anti-human immunodeficiency virus type 1 (HIV-1) therapy and limits treatment options. Therefore, new targets that can be used to develop novel antiviral agents need to be identified. We previously identified a potential parent compound, hematoxylin, which suppresses the nuclear import of HIV-1 via the Vpr-importin α interaction and inhibits HIV-1 replication in a Vpr-dependent manner by blocking nuclear import of the pre-i...
1 CitationsSource
#1Yutaka SasakiH-Index: 5
#2Kyoji HagiwaraH-Index: 5
Last.Yoko Aida (UTokyo: University of Tokyo)H-Index: 29
view all 6 authors...
The nucleoprotein (NP) of influenza A virus is transported into the nucleus via the classical importin α/β pathway, and proceeds via nuclear localization signals (NLSs) recognized by importin α molecules. Although NP binds to importin α isoforms Rch1, Qip1 and NPI-1, the role of each individual isoform during the nuclear transport of NP and replication of the influenza virus remains unknown. In this study, we examined the contribution of importin α isoforms for nuclear localization of NP and vir...
9 CitationsSource
#1Kazunori D. Yamada (UTokyo: University of Tokyo)H-Index: 10
#2Hiroko Koyama (Gidai: Gifu University)H-Index: 11
Last.Yoko Aida (UTokyo: University of Tokyo)H-Index: 29
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Abstract Influenza viruses have developed resistance to current drugs, creating a need for new antiviral targets and new drugs to treat influenza virus infections. In this study, computational and experimental screening of an extensive compound library identified THC19, which was able to suppress influenza virus replication. This compound had no cytotoxic effects and did not disrupt cell cycle progression or induce apoptosis in MDCK cells as confirmed by WST-1 assays, flow cytometry analysis, an...
21 CitationsSource
#1Yoko AidaH-Index: 29
#2Yutaka SasakiH-Index: 5
Last.Kyoji HagiwaraH-Index: 5
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The influenza virus types A, B, and C belong to the family Orthomyxoviridae. Influenza B and C viruses are predominantly human pathogens, whereas influenza A virus spreads not only in humans but also in many animals, including birds and pigs. This characteristic of influenza A viruses is a major cause of influenza pandemics in humans. Influenza A viruses are responsible for periodic widespread epidemics, or pandemics, which have taken the form of respiratory diseases with cold-like symptoms, but...
4 CitationsSource
#1Kyoji HagiwaraH-Index: 5
#2Tomoyuki Murakami (UTokyo: University of Tokyo)H-Index: 6
Last.Yoko Aida (UTokyo: University of Tokyo)H-Index: 29
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Abstract Although HIV-1 replication can be controlled by highly active anti-retroviral therapy (HAART) using protease and reverse transcriptase inhibitors, the development of multidrug-resistant viruses compromises the efficacy of HAART. Thus, it is necessary to develop new drugs with novel targets. To identify new anti-HIV-1 compounds, recombinant Vpr was purified from transfected COS-7 cells and used to screen compounds by chemical array to identify those that bound Vpr. From this screen, 108 ...
15 CitationsSource
#1Kyoji HagiwaraH-Index: 5
#2Yasumitsu KondohH-Index: 16
Last.Yoko Aida (UTokyo: University of Tokyo)H-Index: 29
view all 9 authors...
The nucleoprotein (NP) of the influenza virus is expressed in the early stage of infection and plays important roles in numerous steps of viral replication. NP is relatively well conserved compared with viral surface spike proteins. This study experimentally demonstrates that NP is a novel target for the development of new antiviral drugs against the influenza virus. First, artificial analogs of mycalamide A in a chemical array bound specifically with high affinity to NP. Second, the compounds i...
46 CitationsSource
#1Shigetoshi Miyajima (Mie University)H-Index: 10
#2Akira Mori (Mie University)H-Index: 2
Last.Masahiro Tomita (Mie University)H-Index: 23
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4 CitationsSource
#1Kyoji Hagiwara (Mie University)H-Index: 5
#2Masahiro Tomita (Mie University)H-Index: 23
Last.Tetsuro Yoshimura (Mie University)H-Index: 11
view all 5 authors...
The segments 8 (S8) of the 10 double-stranded RNA genomes from Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) strains I and H were converted into cDNAs, amplified by PCR, and cloned. The nucleotide sequence analysis of the two full-length S8 cDNAs showed that the segments consist of 1328 nucleotides encoding putative proteins (p44) of 390 amino acids with molecular masses of about 44 kDa, which have glutamic acid-rich and proline-rich domains in their central regions. They had quite high ide...
27 CitationsSource