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Hao Wu
Johns Hopkins University School of Medicine
19Publications
12H-index
1,224Citations
Publications 23
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#1Hao WuH-Index: 12
view all 6 authors...
Patients with Prader Willi Syndrome (PWS) display intellectual impairment, hyperphagia, and various behavioral problems during childhood that converge on a neurologic deficit. The majority of PWS patients have genetic deletions of the paternal 15q11-q13 chromosomal region, with their maternal PWS locus intact but epigenetically silenced by hypermethylation and repressive histone modulation of the PWS imprinting center (PWS IC). Inhibition of the euchromatin histone methyltransferase G9a by small...
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#1X. Shawn Liu (MIT: Massachusetts Institute of Technology)H-Index: 2
#2Hao Wu (MIT: Massachusetts Institute of Technology)H-Index: 12
Last.Rudolf Jaenisch (MIT: Massachusetts Institute of Technology)H-Index: 175
view all 15 authors...
Summary Fragile X syndrome (FXS), the most common genetic form of intellectual disability in males, is caused by silencing of the FMR1 gene associated with hypermethylation of the CGG expansion mutation in the 5′ UTR of FMR1 in FXS patients. Here, we applied recently developed DNA methylation editing tools to reverse this hypermethylation event. Targeted demethylation of the CGG expansion by dCas9-Tet1/single guide RNA (sgRNA) switched the heterochromatin status of the upstream FMR1 promoter to ...
83 CitationsSource
#1Hai Huang ('SMMU': Second Military Medical University)H-Index: 1
#2Qin ZhangH-Index: 2
Last.Wen-Hui Liu (SJTU: Shanghai Jiao Tong University)H-Index: 1
view all 11 authors...
Purpose Prostate cancer is one of the leading causes of cancer death for male. In the present study, we applied an integrated bioinformatics approach to provide a novel perspective and identified some hub genes of prostate cancer.
8 CitationsSource
#1Anja Maier (FAU: University of Erlangen-Nuremberg)H-Index: 3
#2Hao Wu (JHUSOM: Johns Hopkins University School of Medicine)H-Index: 12
Last.Christina Warnecke (FAU: University of Erlangen-Nuremberg)H-Index: 23
view all 9 authors...
Recently we identified hypoxia-inducible protein 2 (HIG2)/hypoxia-inducible lipid droplet–associated (HILPDA) as lipid droplet (LD) protein. Because HILPDA is highly expressed in atherosclerotic plaques, we examined its regulation and function in murine macrophages, compared it to the LD adipose differentiation-related protein (Adrp)/perilipin 2 (Plin2), and investigated its effects on atherogenesis in apolipoprotein E–deficient (ApoE−/−) mice. Tie2-Cre-driven Hilpda conditional knockout (cKO) d...
11 CitationsSource
#1Yonatan Stelzer (MIT: Massachusetts Institute of Technology)H-Index: 11
#2Hao Wu (MIT: Massachusetts Institute of Technology)H-Index: 12
Last.Rudolf Jaenisch (MIT: Massachusetts Institute of Technology)H-Index: 175
view all 6 authors...
Summary Parent-specific differentially methylated regions (DMRs) are established during gametogenesis and regulate parent-specific expression of imprinted genes. Monoallelic expression of imprinted genes is essential for development, suggesting that imprints are faithfully maintained in embryos and adults. To test this hypothesis, we targeted a reporter for genomic methylation to the imprinted Dlk1-Dio3 intergenic DMR (IG-DMR) to assess the methylation of both parental alleles at single-cell res...
11 CitationsSource
#1X. Shawn Liu (MIT: Massachusetts Institute of Technology)H-Index: 2
#2Hao Wu (MIT: Massachusetts Institute of Technology)H-Index: 12
Last.Rudolf Jaenisch (MIT: Massachusetts Institute of Technology)H-Index: 175
view all 10 authors...
Mammalian DNA methylation is a critical epigenetic mechanism orchestrating gene expression networks in many biological processes. However, investigation of the functions of specific methylation events remains challenging. Here, we demonstrate that fusion of Tet1 or Dnmt3a with a catalytically inactive Cas9 (dCas9) enables targeted DNA methylation editing. Targeting of the dCas9-Tet1 or -Dnmt3a fusion protein to methylated or unmethylated promoter sequences caused activation or silencing, respect...
380 CitationsSource
#1Yonatan StelzerH-Index: 11
#2Hao WuH-Index: 12
Last.Rudolf JaenischH-Index: 17
view all 6 authors...
#1Beth FriedmanH-Index: 11
#2Lee F. SchroederH-Index: 4
Last.Tristan HedrickH-Index: 7
view all 15 authors...
#1Hao Chang (JHUSOM: Johns Hopkins University School of Medicine)H-Index: 10
#2Yanshu Wang (JHUSOM: Johns Hopkins University School of Medicine)H-Index: 18
Last.Jeremy Nathans (JHUSOM: Johns Hopkins University School of Medicine)H-Index: 83
view all 4 authors...
Skin is a highly heterogeneous tissue. Intra-dermal structures include hair follicles, arrector pili muscles, epidermal specializations (such as Merkel cell clusters), sebaceous glands, nerves and nerve endings, and capillaries. The spatial arrangement of these structures is tightly controlled on a microscopic scale - as seen, for example, in the orderly arrangement of cell types within a single hair follicle - and on a macroscopic scale - as seen by the nearly identical orientations of thousand...
7 CitationsSource
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