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Bo Wang
Northwestern Polytechnical University
4Publications
2H-index
9Citations
Publications 4
Newest
#1Hai Hou (NPU: Northwestern Polytechnical University)H-Index: 3
#2Bo Wang (NPU: Northwestern Polytechnical University)H-Index: 2
Last.Da-Chuan Yin (NPU: Northwestern Polytechnical University)H-Index: 16
view all 7 authors...
Abstract Surface treatment by oxidizing the crystallization plates can significantly promote protein crystallization and requires no change to routine screening protocols; therefore, it is potentially useful for practical protein crystallization screening. However, experiments have shown that the amount of oxidants and the treatment process need to be optimized to achieve effective results. Searching for the suitable amount of oxidants, temperature and processing time for surface treatment of th...
#1Hai Hou (NPU: Northwestern Polytechnical University)H-Index: 3
#2Bo Wang (NPU: Northwestern Polytechnical University)H-Index: 2
Last.Da-Chuan Yin (NPU: Northwestern Polytechnical University)H-Index: 16
view all 6 authors...
We presented a systematic method that combined the cross-diffusion microbatch method (CDM) with the seeding technique to enhance protein crystal reproducibility based on a “common dispersing agent”. The common dispersing agent is used to dilute the crystal seeds. Seven proteins were screened, and more crystallization conditions were found. X-ray diffraction showed that the combined method is practical and useful for obtaining high-quality protein crystals.
#1Hai Hou (NPU: Northwestern Polytechnical University)H-Index: 3
#2Yue Liu (NPU: Northwestern Polytechnical University)H-Index: 2
Last.Da-Chuan Yin (NPU: Northwestern Polytechnical University)H-Index: 16
view all 7 authors...
The quality of protein crystals is an important parameter for structural determination with X-ray crystallography. Indeed, a prerequisite for obtaining high-resolution diffraction data is that the crystals be of sufficient quality. However, obtaining high-quality protein crystals is a well known bottleneck to protein structural determination that remains a difficult task. In this paper, it is demonstrated that recrystallization can be an effective method of improving the quality of protein cryst...
#1Hai Hou (NPU: Northwestern Polytechnical University)H-Index: 1
#2Bo Wang (NPU: Northwestern Polytechnical University)H-Index: 2
Last.Da-Chuan Yin (NPU: Northwestern Polytechnical University)H-Index: 16
view all 8 authors...
We presented a systematic quality comparison of protein crystals grown using the cross-diffusion microbatch (CDM) and standard sitting-drop vapor diffusion methods. Eleven proteins were screened and it was found that crystals grown using CDM exhibited a better morphology. X-ray diffraction showed that the CDM method is practical and useful for obtaining high-quality protein crystals.
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