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Hideyuki Miyatake
34Publications
7H-index
268Citations
Publications 33
Newest
#1Roopa Dharmatti (TITech: Tokyo Institute of Technology)H-Index: 1
#2Hideyuki MiyatakeH-Index: 7
Last.Yoshihiro Ito (TITech: Tokyo Institute of Technology)H-Index: 39
view all 8 authors...
(1) Background: The folate receptor (FR) is a target for cancer treatment and detection. Expression of the FR is restricted in normal cells but overexpressed in many types of tumors. Folate was conjugated with peptides for enhancing binding affinity to the FR. (2) Materials and Methods: For conjugation, folate was coupled with propargyl or dibenzocyclooctyne, and 4-azidophenylalanine was introduced in peptides for “click” reactions. We measured binding kinetics including the rate constants of as...
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#1Xueli Ren (JLU: Jilin University)H-Index: 1
#2Jun AkimotoH-Index: 8
Last.Yoshihiro ItoH-Index: 39
view all 11 authors...
Vascular endothelial growth factors (VEGFs) play an important role in tissue repair and regeneration. In this study, VEGFs were immobilised by photo-reactive gelatine by photo-irradiation and the effect of immobilisation was quantitatively investigated. Immobilised VEGFs enhanced endothelial cell migration and growth more than soluble VEGFs. In addition, the activation effects depended on the VEGF isoforms. Prolonged activation of phosphorylation sites in the VEGF receptor, which is associated w...
1 CitationsSource
#1Krishnachary Salikolimi (Tokyo Metropolitan University)H-Index: 1
#2Hideyuki MiyatakeH-Index: 7
Last.Yoshihiro Ito (Tokyo Metropolitan University)H-Index: 39
view all 5 authors...
A new type of turn-on electrochemical protein detection is developed using an electropolymerizable molecular probe. To detect trypsin, a benzamidine ligand is conjugated with a thiophene moiety. Encapsulation of the probe in the trypsin pocket prevents electropolymerization, leading to efficient electron transfer from the electrolyte to the electrode. In contrast, unbound probes can become electropolymerized, yielding a polythiophene layer on the electrode. The polythiophene formed this way supp...
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#1Roopa Dharmatti (TITech: Tokyo Institute of Technology)H-Index: 1
#2Hideyuki MiyatakeH-Index: 7
Last.Yoshihiro Ito (TITech: Tokyo Institute of Technology)H-Index: 39
view all 8 authors...
Abstract Human folate receptors (hFRα and hFRβ) are membrane proteins anchored to the cell surface by glycosylphosphatidylinositol. They play an important role in cell growth by taking up folate for de novo synthesis of purines and methylation of DNA, lipids, and proteins. Thus, controlling folate uptake through hFRs may lead to the development of anti-cancer drugs. Development of hFRs-targeting drug requires a large amount of hFRs. However, it is difficult to prepare active forms of hFRs from p...
1 CitationsSource
#1Seiichi TadaH-Index: 12
#2Hideyuki MiyatakeH-Index: 7
Last.Yoshihiro ItoH-Index: 39
view all 4 authors...
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#1Roopa DharmattiH-Index: 1
#2Hideyuki MiyatakeH-Index: 7
Last.Yoshihiro ItoH-Index: 39
view all 3 authors...
#1Xueli RenH-Index: 1
#2Hideyuki MiyatakeH-Index: 7
Last.Yoshihiro ItoH-Index: 39
view all 3 authors...
The inositol 1,4,5-trisphosphate (IP 3 ) receptor (IP 3 R) is an IP 3 -gated ion channel that releases calcium ions (Ca 2+ ) from the endoplasmic reticulum. The IP 3 -binding sites in the large cytosolic domain are distant from the Ca 2+ conducting pore, and the allosteric mechanism of how IP 3 opens the Ca 2+ channel remains elusive. Here, we identify a long-range gating mechanism uncovered by channel mutagenesis and X-ray crystallography of the large cytosolic domain of mouse type 1 IP 3 R in ...
29 CitationsSource
#1Kozo HamadaH-Index: 14
#2Hideyuki MiyatakeH-Index: 7
Last.Katsuhiko MikoshibaH-Index: 109
view all 4 authors...
Source
#1Kozo HamadaH-Index: 14
#2Hideyuki MiyatakeH-Index: 7
Last.Katsuhiko MikoshibaH-Index: 109
view all 4 authors...
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