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Erwei Zuo
Chinese Academy of Sciences
8Publications
6H-index
187Citations
Publications 10
Newest
#1Erwei ZuoH-Index: 6
#2Yidi Sun (CAS: Chinese Academy of Sciences)H-Index: 8
Last.LIYixue (CAS: Chinese Academy of Sciences)H-Index: 57
view all 11 authors...
Base editors hold promise for correcting pathogenic mutations, while substantial single nucleotide variations (SNVs) on both DNA and RNA were generated by cytosine base editors (CBEs). Here we examined possibilities to reduce off-target effects by engineering cytosine deaminases. By screening 24 CBEs harboring various rAPOBEC1 (BE3) or human APOBEC3A (BE3-hA3A) mutations on the ssDNA or RNA binding domain, we found 8 CBE variations could maintain high on-target editing efficiency. Using Genome-w...
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#1Jin-Jing Li (Fujian Medical University)H-Index: 2
#2Shunyan Hong (Fujian Medical University)
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 29
view all 5 authors...
Abstract CRISPR-mediated genome editing is a revolutionary technology for genome manipulation that utilizes the CRISPR-Cas systems and base editors. Currently, poor efficiency and off-target problems have impeded the application of CRISPR systems. The on-target efficiency has been improved in several advanced versions of CRISPR systems, whereas the off-target detection still remains a key challenge. Here, we outline the different versions of CRISPR systems and off-target detection strategies, di...
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#1Jin-Jing Li (Fujian Medical University)H-Index: 2
#2Xiang Lin (Fujian Medical University)H-Index: 3
Last.Wan-Jin Chen (Fujian Medical University)H-Index: 11
view all 24 authors...
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#1Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
#2Yidi Sun (CAS: Chinese Academy of Sciences)H-Index: 8
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 14 authors...
Recently developed DNA base editing methods enable the direct generation of desired point mutations in genomic DNA without generating any double-strand breaks1–3, but the issue of off-target edits has limited the application of these methods. Although several previous studies have evaluated off-target mutations in genomic DNA4–8, it is now clear that the deaminases that are integral to commonly used DNA base editors often bind to RNA9–13. For example, the cytosine deaminase APOBEC1—which is used...
16 CitationsSource
#1Erwei Zuo (CAS: Chinese Academy of Sciences)H-Index: 6
#2Yidi Sun (CAS-MPG Partner Institute for Computational Biology)H-Index: 8
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 10 authors...
Genome editing holds promise for correcting pathogenic mutations. However, it is difficult to determine off-target effects of editing due to single nucleotide polymorphism in individuals. Here, we developed a method named GOTI (Genome-wide Off-target analysis by Two-cell embryo Injection) to detect off-target mutations by editing one blastomere of two-cell mouse embryos using either CRISPR-Cas9 or base editors. Comparison of the whole genome sequences of progeny cells of edited vs. non-edited bl...
66 CitationsSource
#1Hui YangH-Index: 19
#2LIYixueH-Index: 57
Last.Lars M. Steinmetz (Stanford University)H-Index: 56
view all 8 authors...
Genome editing tools including CRISPR/Cas9 and base editors hold great promise for correcting pathogenic mutations. Unbiased genome-wide off-target effects of the editing in mammalian cells is required before clinical applications, but determination of the extent of off-target effects has been difficult due to the existence of single nucleotide polymorphisms (SNPs) in individuals. Here, we developed a method named GOTI (Genome-wide Off-target analysis by Two-cell embryo Injection) to detect off-...
4 CitationsSource
#1Erwei Zuo (CAS: Chinese Academy of Sciences)H-Index: 6
#2Xiaona Huo (CAS: Chinese Academy of Sciences)H-Index: 2
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 18 authors...
The CRISPR/Cas9 system has become an efficient gene editing method for generating cells carrying precise gene mutations, including the rearrangement and deletion of chromosomal segments. However, whether an entire chromosome could be eliminated by this technology is still unknown. Here we demonstrate the use of the CRISPR/Cas9 system to eliminate targeted chromosomes. Using either multiple cleavages induced by a single-guide RNA (sgRNA) that targets multiple chromosome-specific sites or a cockta...
34 CitationsSource
#1Erwei Zuo (CAS: Chinese Academy of Sciences)H-Index: 6
#2Yijun Cai (CAS: Chinese Academy of Sciences)H-Index: 8
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 29 authors...
One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs
47 CitationsSource
#1Lingbo Wang (CAS: Chinese Academy of Sciences)H-Index: 6
#2Min Yin Li (CAS: Chinese Academy of Sciences)H-Index: 2
Last.Jinsong Li (CAS: Chinese Academy of Sciences)H-Index: 29
view all 22 authors...
CRISPR-Cas9-mediated genome editing in one blastomere of two-cell embryos reveals a novel Tet3 function in regulating neocortical development
9 CitationsSource
#1Man ZhangH-Index: 7
#2Hai ZhouH-Index: 2
Last.Jinsong LiH-Index: 29
view all 12 authors...
C-kit positive (c-kit(+)) cells are usual tissue-specific stem cells. However, in postnatal testis, undifferentiated spermatogonial stem cells (SSCs) are c-kit negative (c-kit(-)) and activation of c-kit represents the start of SSC differentiation, leaving an intriguing question whether other c-kit(+) cells exist and participate in the postnatal development of testis. To this end, a feasible system for testicular reconstitution, in which a specific type of cells can be manipulated, is needed. He...
18 CitationsSource
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