Erwei Zuo
Chinese Academy of Sciences
GeneGenome editingGeneticsBiologyCRISPR
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Publications 20
#1Sangsin Lee (Rice University)
#2Ning Ding (Rice University)
Last. Anatoly B. Kolomeisky (Rice University)H-Index: 40
view all 13 authors...
Cytosine base editors (CBEs) enable efficient cytidine-to-thymidine (C-to-T) substitutions at targeted loci without double-stranded breaks. However, current CBEs edit all Cs within their activity windows, generating undesired bystander mutations. In the most challenging circumstance, when a bystander C is adjacent to the targeted C, existing base editors fail to discriminate them and edit both Cs. To improve the precision of CBE, we identified and engineered the human APOBEC3G (A3G) deaminase; w...
#1Erwei Zuo (CAS: Chinese Academy of Sciences)H-Index: 7
#2Yidi Sun (CAS: Chinese Academy of Sciences)H-Index: 8
Last. Yixue LiH-Index: 59
view all 11 authors...
Cytosine base editors (CBEs) offer a powerful tool for correcting point mutations, yet their DNA and RNA off-target activities have caused concerns in biomedical applications. We describe screens of 23 rationally engineered CBE variants, which reveal mutation residues in the predicted DNA-binding site can dramatically decrease the Cas9-independent off-target effects. Furthermore, we obtained a CBE variant—YE1-BE3-FNLS—that retains high on-target editing efficiency while causing extremely low off...
3 CitationsSource
#1Xiukun WangH-Index: 3
#2Jun-Yan KangH-Index: 3
Last. Yanyan ChenH-Index: 3
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A reader alerted the journal to duplicated images in Fig. 2C and Fig. 4D of Development (2019) 146, [dev175547][1] ([doi:10.1242/dev.175547][2]). In the top panel of Fig. 4D, the bottom part of Stage I and the top part of Stage II-III images show an overlap. This is permissible since
#1Xiang Lin (Fujian Medical University)H-Index: 4
#2Hai-Zhu Chen (Fujian Medical University)H-Index: 1
Last. Wenqin Ying (CAS: Chinese Academy of Sciences)H-Index: 8
view all 15 authors...
#1Erwei Zuo (CAS: Chinese Academy of Sciences)H-Index: 7
#2Yidi Sun (CAS: Chinese Academy of Sciences)H-Index: 8
Last. LIYixue
view all 11 authors...
Base editors hold promise for correcting pathogenic mutations, while substantial single nucleotide variations (SNVs) on both DNA and RNA were generated by cytosine base editors (CBEs). Here we examined possibilities to reduce off-target effects by engineering cytosine deaminases. By screening 24 CBEs harboring various rAPOBEC1 (BE3) or human APOBEC3A (BE3-hA3A) mutations on the ssDNA or RNA binding domain, we found 8 CBE variations could maintain high on-target editing efficiency. Using Genome-w...
3 CitationsSource
#1Jin-Jing Li (Fujian Medical University)H-Index: 4
#2Xiang Lin (Fujian Medical University)H-Index: 4
Last. Wan-Jin Chen (Fujian Medical University)H-Index: 11
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We here report a genome-editing strategy to correct spinal muscular atrophy (SMA). Rather than directly targeting the pathogenic exonic mutations, our strategy employed Cas9 and guide-sgRNA for the targeted disruption of intronic splicing-regulatory elements. We disrupted intronic splicing silencers (ISSs, including ISS-N1 and ISS + 100) of survival motor neuron (SMN) 2, a key modifier gene of SMA, to enhance exon 7 inclusion and full-length SMN expression in SMA iPSCs. Survival of splicing-corr...
1 CitationsSource
#1Jinjing Li (Fujian Medical University)H-Index: 1
#1Jin-Jing Li (Fujian Medical University)H-Index: 4
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 53
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Abstract CRISPR-mediated genome editing is a revolutionary technology for genome manipulation that utilizes the CRISPR-Cas systems and base editors. Currently, poor efficiency and off-target problems have impeded the application of CRISPR systems. The on-target efficiency has been improved in several advanced versions of CRISPR systems, whereas the off-target detection still remains a key challenge. Here, we outline the different versions of CRISPR systems and off-target detection strategies, di...
5 CitationsSource
#1Xiu-Ling Zhao (Xida: Guangxi University)H-Index: 1
#1Xiuling Zhao (Xida: Guangxi University)H-Index: 1
Last. Ming Zhang (Xida: Guangxi University)H-Index: 4
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Although numerous attempts have been made to alter the sex ratio of the progeny of mammals, the limitations of current technologies have prevented their widespread use in farm animals. The presence or absence of a Y chromosome determines whether a mammalian embryo develops as a male or female, and non-invasive genetic reporters such as fluorescence protein markers have been intensively applied in a variety of fields of research. To develop a non-invasive and instantaneous method for advance dete...
1 CitationsSource
#1Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
#2Yidi Sun (CAS: Chinese Academy of Sciences)H-Index: 8
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 14 authors...
Recently developed DNA base editing methods enable the direct generation of desired point mutations in genomic DNA without generating any double-strand breaks1–3, but the issue of off-target edits has limited the application of these methods. Although several previous studies have evaluated off-target mutations in genomic DNA4–8, it is now clear that the deaminases that are integral to commonly used DNA base editors often bind to RNA9–13. For example, the cytosine deaminase APOBEC1—which is used...
65 CitationsSource