Andrei Kirillov
Hebrew University of Jerusalem
EnhancerMolecular biologyDemethylationAllelic exclusionBiology
What is this?
Publications 5
#1Rena Levin-Klein (HUJI: Hebrew University of Jerusalem)H-Index: 5
#2Andrei Kirillov (HUJI: Hebrew University of Jerusalem)H-Index: 5
Last. Yehudit Bergman (HUJI: Hebrew University of Jerusalem)H-Index: 37
view all 5 authors...
The Igκ locus undergoes a variety of different molecular processes during B cell development, including V(D)J rearrangement and somatic hypermutations, which are influenced by cis regulatory regions (RR) within the locus. The Igκ locus includes three characterized RRs termed the intronic (iEκ), 3’Eκ and Ed enhancers. We had previously noted that a region of DNA upstream of the iEκ and matrix attachment region (MAR) was necessary for demethylation of the locus in cell culture. In this study, we f...
5 CitationsSource
17 CitationsSource
#1Raul MostoslovaskyH-Index: 50
#2Nandita SinghH-Index: 5
Last. Yehudit BergmanH-Index: 37
view all 7 authors...
Allelic exclusion in κ light-chain synthesis is thought to result from a feedback mechanism by which the expression of a functional κ light chain on the surface of the B cell leads to an intracellular signal that down-regulates the V(D)J recombinase, thus precluding rearrangement of the other allele. Whereas such a feedback mechanism clearly plays a role in the maintenance of allelic exclusion, here we provide evidence suggesting that the initial establishment of allelic exclusion involves diffe...
195 CitationsSource
#1Bernd Baumann (HUJI: Hebrew University of Jerusalem)H-Index: 1
#2Barbara Kistler (HUJI: Hebrew University of Jerusalem)H-Index: 8
Last. Thomas Wirth (HUJI: Hebrew University of Jerusalem)H-Index: 1
view all 5 authors...
Abstract Studies on the mechanisms of inducible and constitutive activity of NF-κB transcription factors have been hampered by the lack of appropriate mutant cell lines. We have analyzed the defect in the murine S107 plasmacytoma cell line, which was previously found to lack both constitutive and inducible NF-κB activity. Our analysis shows that these cells bear a specific defect that interferes with NF-κB induction by many diverse stimuli, such as lipopolysaccharide, phorbol 12-myristate 13-ace...
25 CitationsSource
#1Andrei Kirillov (HUJI: Hebrew University of Jerusalem)H-Index: 5
#2Barbara KistlerH-Index: 8
Last. Yehudit Bergman (HUJI: Hebrew University of Jerusalem)H-Index: 37
view all 6 authors...
The immunoglobulin κ gene is specifically demethylated during B–cell maturation in a process which utilizes discrete cis–acting modules such as the intronic κ enhancer element and the matrix attachment region (MAR). While any MAR sequence is sufficient for this reaction, mutation analysis indicates that tissue specificity is mediated by κB binding sequences within the κ intronic enhancer. The plasmacytoma cell line S107 lacks κB binding activity and fails to demethylate the κ locus. However, B–c...
194 CitationsSource