Match!
Hong Wang
Kunming University of Science and Technology
27Publications
11H-index
1,138Citations
Publications 22
Newest
#1Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
#2Nianqin Sun (Kunming University of Science and Technology)H-Index: 1
Last.Tao Tan (Kunming University of Science and Technology)H-Index: 10
view all 24 authors...
The transition from peri-implantation to gastrulation in mammals entails the specification and organization of the lineage progenitors into a body plan. Technical and ethical challenges have limited understanding of the cellular and molecular mechanisms underlying this transition. Here, we established a culture system that enabled the development of cynomolgus monkey embryos in vitro for up to 20 days. Cultured embryos underwent key primate developmental stages, including lineage segregation, bi...
Source
#1Lei Shi (KIZ: Kunming Institute of Zoology)H-Index: 10
#2Xin LuoH-Index: 2
Last.Bing Su (KIZ: Kunming Institute of Zoology)H-Index: 23
view all 20 authors...
Brain size and cognitive skills are the most dramatically changed traits in humans during evolution and yet the genetic mechanisms underlying these human-specific changes remain elusive. Here, we successfully generated 11 transgenic rhesus monkeys (8 first-generation and 3 second-generation) carrying human copies of MCPH1, an important gene for brain development and brain evolution. Brain-image and tissue-section analyses indicated an altered pattern of neural-cell differentiation, resulting in ...
5 CitationsSource
#1Yiqiang Cui (Nanjing Medical University)H-Index: 11
#2Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
Last.Weizhi Ji (Kunming University of Science and Technology)H-Index: 25
view all 15 authors...
Generation of a precise Oct4-hrGFP knockin cynomolgus monkey model via CRISPR/Cas9-assisted homologous recombination
13 CitationsSource
#1Xufeng Fu (Kunming University of Science and Technology)H-Index: 1
#2Bin Jiang (UM: University of Macau)H-Index: 4
Last.Wei Si (Kunming University of Science and Technology)H-Index: 12
view all 14 authors...
3 CitationsSource
#1Bingbing ChenH-Index: 2
#2Shanshan LiH-Index: 2
Last.Wei SiH-Index: 12
view all 7 authors...
Source
#1Bingbing Chen (Kunming University of Science and Technology)H-Index: 2
#2Shanshan Li (Kunming University of Science and Technology)H-Index: 2
Last.Wei Si (Kunming University of Science and Technology)H-Index: 12
view all 9 authors...
Abstract The cryoprotective agent (CPA) is one of the most important factors that affects the cryosurvival of sperm. The aim of the present study was to compare two different CPAs, glycerol (Gly) and ethylene glycol (EG), on the cryopreservation of cynomolgus macaques sperm and evaluate the effects of cryopreservation on sperm motility, acrosomal integrity, DNA integrity, mitochondrial function and the sperm membrane ion channels CatSper and Hv1. Compared to fresh sperm, cryopreservation with ei...
2 CitationsSource
#1Fei GaoH-Index: 13
#2Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
Last.Tao Tan (Kunming University of Science and Technology)H-Index: 10
view all 16 authors...
Abstract Critical epigenetic regulation of primate embryogenesis entails DNA methylome changes. Here we report genome-wide composition, patterning, and stage-specific dynamics of DNA methylation in pre-implantation rhesus monkey embryos as well as male and female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. We show that upon fertilization, both paternal and maternal genomes undergo active DNA demethylation, and genome-wide de novo DNA methylatio...
15 CitationsSource
#1Xufeng FuH-Index: 1
#2Yaping YanH-Index: 3
Last.Wei SiH-Index: 12
view all 14 authors...
Mesenchymal stem cells (MSCs) are one of the most promising adult stem cells for clinical application in a cell therapy. The development of large-scale cryopreservation techniques, such as vitrification, for MSCs is a prerequisite for clinical therapies. Dimethyl sulfoxide (DMSO) and ethylene glycol (EG) are two types of cryoprotectants widely used for cell vitrification. However, the effects of DMSO and EG on the biological characteristics and transcriptome profiles of MSCs after cryopreservati...
1 CitationsSource
#1Zongyong Ai (Kunming University of Science and Technology)H-Index: 5
#2Zheng Xiang (Kunming University of Science and Technology)H-Index: 4
Last.Tianqing Li (Kunming University of Science and Technology)H-Index: 9
view all 12 authors...
Non-human primates provide optimal models for the development of stem cell therapies. Although somatic cells have been converted into neural stem/progenitor cells, it is unclear whether telencephalic neuroepithelial stem cells (NESCs) with stable properties can be generated from fibroblasts in primate. Here we report that a combination of transcription factors (Oct4, Sox2, Klf4) with a new culture medium induces rhesus monkey fibroblasts into NESCs, which can develop into miniature neural tube (...
4 CitationsSource
#1Yu Kang (Kunming University of Science and Technology)H-Index: 8
#2Bo Zheng (Nanjing Medical University)H-Index: 9
Last.Xingxu Huang (ShanghaiTech University)H-Index: 19
view all 16 authors...
Mutations in the DAX1 locus cause X-linked adrenal hypoplasia congenita (AHC) and hypogonadotropic hypogonadism (HH), which manifest with primary adrenal insufficiency and incomplete or absent sexual maturation, respectively. The associated defects in spermatogenesis can range from spermatogenic arrest to Sertoli cell only syndrome. Conclusions from Dax1 knockout mouse models provide only limited insight into AHC/HH disease mechanisms, because mouse models exhibit more extensive abnormalities in...
40 CitationsSource
123