Jianying Wang
Nanjing Medical University
Publications 13
#1Dali Han (CAS: Chinese Academy of Sciences)H-Index: 18
#2Jun Liu (U of C: University of Chicago)H-Index: 1
Last.Chuan He (U of C: University of Chicago)H-Index: 93
view all 15 authors...
In this Letter, a citation to ‘Fig. 1e’ has been corrected to ‘Fig. 1d’ in the sentence starting “By contrast, the anti-tumour response…”. This has been corrected online
1 CitationsSource
#1Dali Han (CAS: Chinese Academy of Sciences)H-Index: 18
#2Jun Liu (U of C: University of Chicago)H-Index: 1
Last.Chuan He (U of C: University of Chicago)H-Index: 93
view all 15 authors...
There is growing evidence that tumour neoantigens have important roles in generating spontaneous antitumour immune responses and predicting clinical responses to immunotherapies1,2. Despite the presence of numerous neoantigens in patients, complete tumour elimination is rare, owing to failures in mounting a sufficient and lasting antitumour immune response3,4. Here we show that durable neoantigen-specific immunity is regulated by mRNA N6-methyadenosine (m6A) methylation through the m6A-binding p...
14 CitationsSource
#1Liqun Lei (CAS: Chinese Academy of Sciences)H-Index: 2
#2Hongquan Chen (WMU: Wenzhou Medical College)H-Index: 1
Last.Jia Chen (ShanghaiTech University)H-Index: 13
view all 19 authors...
The APOBEC-AID family of cytidine deaminase prefers single-stranded nucleic acids for cytidine-to-uridine deamination. Single-stranded nucleic acids are commonly involved in the DNA repair system for breaks generated by CRISPR–Cas9. Here, we show in human cells that APOBEC3 can trigger cytidine deamination of single-stranded oligodeoxynucleotides, which ultimately results in base substitution mutations in genomic DNA through homology-directed repair (HDR) of Cas9-generated double-strand breaks. ...
9 CitationsSource
#1Phillip J. Hsu (U of C: University of Chicago)H-Index: 7
#2Yunfei Zhu (Nanjing Medical University)H-Index: 2
Last.Chuan He (U of C: University of Chicago)H-Index: 93
view all 18 authors...
N6-methyladenosine (m6A) is the most common internal modification in eukaryotic mRNA. It is dynamically installed and removed, and acts as a new layer of mRNA metabolism, regulating biological processes including stem cell pluripotency, cell differentiation, and energy homeostasis. m6A is recognized by selective binding proteins; YTHDF1 and YTHDF3 work in concert to affect the translation of m6A-containing mRNAs, YTHDF2 expedites mRNA decay, and YTHDC1 affects the nuclear processing of its targe...
92 CitationsSource
#1Yu Kang (Kunming University of Science and Technology)H-Index: 8
#2Bo Zheng (Nanjing Medical University)H-Index: 9
Last.Xingxu Huang (ShanghaiTech University)H-Index: 19
view all 16 authors...
Mutations in the DAX1 locus cause X-linked adrenal hypoplasia congenita (AHC) and hypogonadotropic hypogonadism (HH), which manifest with primary adrenal insufficiency and incomplete or absent sexual maturation, respectively. The associated defects in spermatogenesis can range from spermatogenic arrest to Sertoli cell only syndrome. Conclusions from Dax1 knockout mouse models provide only limited insight into AHC/HH disease mechanisms, because mouse models exhibit more extensive abnormalities in...
40 CitationsSource
#1Yongchang Chen (Kunming University of Science and Technology)H-Index: 13
#2Yiqiang Cui (Nanjing Medical University)H-Index: 11
Last.Xingxu Huang (National Resource Center)H-Index: 19
view all 12 authors...
19 CitationsSource
#1Jiankui Zhou (National Resource Center)H-Index: 5
#2Jianying Wang (National Resource Center)H-Index: 10
Last.Xingxu Huang (National Resource Center)H-Index: 19
view all 9 authors...
The bacterial clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system is a versatile RNA-guided mammalian genome modification system. One-step generation of mouse genome targeting has been achieved by co-microinjection of one-cell stage embryos with Cas9 mRNA and small/single guide (sg)RNA. Many studies have focused on enhancing the efficiency of this system. In the present study, we report that simultaneous use of dual sgRNAs to target an individual ...
74 CitationsSource
#1Bin ShenH-Index: 3
#2Wensheng ZhangH-Index: 2
Last.William C. SkarnesH-Index: 53
view all 11 authors...
This paper describes the use of paired Cas9 nickases to edit the mammalian genome with no detectable off-target effects.
432 CitationsSource
#1Ke Ma (TMUCIH: Tianjin Medical University Cancer Institute and Hospital)H-Index: 3
#2Jianying Wang (National Resource Center)H-Index: 10
Last.Zheng Li (TMUCIH: Tianjin Medical University Cancer Institute and Hospital)H-Index: 4
view all 6 authors...
Transcription activator-like effector nucleases (TALENs) have emerged as a newly developed approach for genome editing. However, its application in targeting specific genomic loci susceptible to DNA damage remains obscure. Here, we report a modified approach for TALENs-based targeting of FATS, a fragile-site gene whose major introns have AT-rich sequence and di-nucleotide repeats. Two pairs of FATS–TALENs were designed to cleave two sites specifically at a coding exon of FATS. After in vitro tra...
6 CitationsSource
#1Yuyu NiuH-Index: 17
#2Bin Shen (National Resource Center)H-Index: 18
Last.Jiahao Sha (Nanjing Medical University)H-Index: 34
view all 28 authors...
Summary Monkeys serve as important model species for studying human diseases and developing therapeutic strategies, yet the application of monkeys in biomedical researches has been significantly hindered by the difficulties in producing animals genetically modified at the desired target sites. Here, we first applied the CRISPR/Cas9 system, a versatile tool for editing the genes of different organisms, to target monkey genomes. By coinjection of Cas9 mRNA and sgRNAs into one-cell-stage embryos, w...
628 CitationsSource