Brandon C. Knott
National Renewable Energy Laboratory
Publications 23
#1Roman Brunecky (NREL: National Renewable Energy Laboratory)H-Index: 16
#2Venkataramanan Subramanian (NREL: National Renewable Energy Laboratory)H-Index: 14
Last.Stephen R. Decker (NREL: National Renewable Energy Laboratory)H-Index: 31
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Significant effort has been expended toward the discovery and/or engineering of improved cellulases. An alternative to this approach is utilizing multifunctional enzymes; however, essentially most if not all relevant bacterial enzymes of this type do not express well in fungi. Therefore, developing a systematic understanding of how to construct multifunctional enzymes that are expressible in commercial fungal hosts is crucial to developing next generation enzymes for biomass deconstruction. Mult...
Cellulase enzymes deconstruct recalcitrant cellulose into soluble sugars, making them a biocatalyst of biotechnological interest for use in the nascent lignocellulosic bioeconomy. Cellobiohydrolases (CBHs) are cellulases capable of liberating many sugar molecules in a processive manner without dissociating from the substrate. Within the complete processive cycle of CBHs, dissociation from the cellulose substrate is rate limiting, but the molecular mechanism of this step is unknown. Here, we pres...
#2Lintao BuH-Index: 12
Last.Seonah KimH-Index: 15
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#1Melodie M. Machovina (MSU: Montana State University)H-Index: 3
#2S.J.B. Mallinson (University of Portsmouth)H-Index: 1
Last.Jennifer L. DuBois (MSU: Montana State University)H-Index: 17
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Microbial conversion of aromatic compounds is an emerging and promising strategy for valorization of the plant biopolymer lignin. A critical and often rate-limiting reaction in aromatic catabolism is O-aryl-demethylation of the abundant aromatic methoxy groups in lignin to form diols, which enables subsequent oxidative aromatic ring-opening. Recently, a cytochrome P450 system, GcoAB, was discovered to demethylate guaiacol (2-methoxyphenol), which can be produced from coniferyl alcohol-derived li...
#1Daehwan Chung (NREL: National Renewable Energy Laboratory)H-Index: 5
#2Nicholas S. Sarai (California Institute of Technology)H-Index: 1
Last.Yannick J. Bomble (NREL: National Renewable Energy Laboratory)H-Index: 23
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In the terrestrial biosphere, biomass deconstruction is conducted by microbes employing a variety of complementary strategies, many of which remain to be discovered. Moreover, the biofuels industry seeks more efficient (and less costly) cellulase formulations upon which to launch the nascent sustainable bioenergy economy. The glycan decoration of fungal cellulases has been shown to protect these enzymes from protease action and to enhance binding to cellulose. We show here that thermal tolerant ...
1 CitationsSource
#1Larry E. Taylor (NREL: National Renewable Energy Laboratory)H-Index: 13
#2Brandon C. Knott (NREL: National Renewable Energy Laboratory)H-Index: 12
Last.Gregg T. Beckham (NREL: National Renewable Energy Laboratory)H-Index: 50
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Glycoside Hydrolase Family 7 cellobiohydrolases (GH7 CBHs) catalyze cellulose depolymerization in cellulolytic eukaryotes, making them key discovery and engineering targets. However, there remains a lack of robust structure–activity relationships for these industrially important cellulases. Here, we compare CBHs from Trichoderma reesei (TrCel7A) and Penicillium funiculosum (PfCel7A), which exhibit a multi-modular architecture consisting of catalytic domain (CD), carbohydrate-binding module, and ...
9 CitationsSource
#1Brandon C. KnottH-Index: 12
#2Claire T. NimlosH-Index: 2
Last.Rajamani GounderH-Index: 23
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Research efforts in zeolite catalysis have become increasingly cognizant of the diversity in structure and function resulting from the distribution of framework aluminum atoms, through emerging reports of catalytic phenomena that fall outside those recognizable as the shape-selective ones emblematic of its earlier history. Molecular-level descriptions of how active-site distributions affect catalysis are an aspirational goal articulated frequently in experimental and theoretical research, yet th...
21 CitationsSource
#1Antonella Amore (NREL: National Renewable Energy Laboratory)H-Index: 15
#2Brandon C. Knott (NREL: National Renewable Energy Laboratory)H-Index: 12
Last.Larry E. Taylor (NREL: National Renewable Energy Laboratory)H-Index: 13
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In nature, many microbes secrete mixtures of glycoside hydrolases, oxidoreductases, and accessory enzymes to deconstruct polysaccharides and lignin in plants. These enzymes are often decorated with N- and O-glycosylation, the roles of which have been broadly attributed to protection from proteolysis, as the extracellular milieu is an aggressive environment. Glycosylation has been shown to sometimes affect activity, but these effects are not fully understood. Here, we examine N- and O-glycosylati...
15 CitationsSource
Biomass pyrolysis offers a promising means to rapidly depolymerize lignocellulosic biomass for subsequent catalytic upgrading to renewable fuels. Substantial efforts are currently ongoing to optimize pyrolysis processes including various fast pyrolysis and catalytic fast pyrolysis schemes. In all cases, complex aqueous streams are generated containing solubilized organic compounds that are not converted to target fuels or chemicals and are often slated for wastewater treatment, in turn creating ...
14 CitationsSource
#1Sarah E. Hobdey (NREL: National Renewable Energy Laboratory)H-Index: 7
#2Brandon C. Knott (NREL: National Renewable Energy Laboratory)H-Index: 12
Last.Jerry Ståhlberg (SLU: Swedish University of Agricultural Sciences)H-Index: 38
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ABSTRACT Glycoside hydrolase family 7 (GH7) cellobiohydrolases (CBHs) are enzymes commonly employed in plant cell wall degradation across eukaryotic kingdoms of life, as they provide significant hydrolytic potential in cellulose turnover. To date, many fungal GH7 CBHs have been examined, yet many questions regarding structure-activity relationships in these important natural and commercial enzymes remain. Here, we present the crystal structures and a biochemical analysis of two GH7 CBHs from soc...
5 CitationsSource