Elizaveta V. Koudan
Composite materialSpheroidMaterials sciencePolyurethaneBiofabrication
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Publications 18
#1V. A. Parfenov (RAS: Russian Academy of Sciences)H-Index: 5
#2Vladimir MironovH-Index: 8
Last. I. V. VakhrushevH-Index: 9
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The calcium phosphate particles can be used as building blocks for fabrication of 3D scaffolds intended for bone tissue engineering. This work presents for the first time a rapid creation of 3D scaffolds using magnetic levitation of calcium phosphate particles. Namely, tricalcium phosphate particles of equal size and certain porosity are used, which undergo the process of recrystallization after magnetic levitational assembly of the scaffold to ensure stitching of the scaffold. Label-free levita...
#1V. A. ParfenovH-Index: 5
#2Vladimir MironovH-Index: 8
Last. M. I. Myasnikov (RAS: Russian Academy of Sciences)H-Index: 6
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The feasibility of magnetic levitational bioassembly of tissue engineered constructs from living tissue spheroids in the presence of paramagnetic ions (i.e. Gd3+) was recently demonstrated. However, Gd3+ is relatively toxic at concentrations above 50 mM normally used to enable magnetic levitation with NdFeB-permanent magnets. Using a high magnetic field (a 50 mm-bore, 31 T Bitter magnet) in High Field Magnet Laboratory in Radboud University in Nijmegen, the Netherlands, we performed magnetic lev...
#2Anna A. Gryadunova (MSMU: I.M. Sechenov First Moscow State Medical University)H-Index: 3
Last. Frederico Pereira D.A.S.H-Index: 9
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Reproducible, scalable, and cost effective fabrication and versatile characterization of tissue spheroids (TS) is highly demanded by 3D bioprinting and drug discovery. Consistent geometry, defined mechanical properties, optimal viability, appropriate extracellular matrix/cell organization are required for cell aggregates aimed for application in these fields. A straightforward procedure for fabrication and systematic multiparametric characterization of TS with defined properties and uniform pred...
#1E. O. Osidak (IMTEK)H-Index: 3
#2Pavel A. KaralkinH-Index: 1
Last. S. P. Domogatsky (IMTEK)H-Index: 1
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Collagen is one of the most promising materials for 3D bioprinting because of its distinguished biocompatibility. Cell-laden constructs made of pure collagen with or without incorporated growth supplements support engineered constructs persistence in culture and are perfectly suitable for grafting. The limiting factor for direct 3D collagen printing was poor printability of collagen solutions, especially admixed with cells or tissue spheroids. In our study, we showed that concentrated solutions ...
1 CitationsSource
#1Anton E. EfimovH-Index: 6
#2O. I. AgapovaH-Index: 3
Last. Igor I. AgapovH-Index: 16
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#1Anna A. GryadunovaH-Index: 3
#3E. A. BulanovaH-Index: 7
Last. Vladimir MironovH-Index: 8
view all 6 authors...
#2Oleg A. SapozhnikovH-Index: 29
Last. V. A. ParfenovH-Index: 5
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This paper presents the method of magneto-acoustic biofabrication of a bioengineering construct from tissue spheroids. It allows creating tissue constructs of tubular shapes. Collected together in a magneto-acoustic trap, tissue spheroids contact with each other and thus fuse to form a 3D tissue construct. The magnetic system was made of two oppositely oriented permanent magnets with an empty space between them. A cylindrical ultrasonic transducer was placed into this space, and a container with...
Last. Vladimir Mironov (MSMU: I.M. Sechenov First Moscow State Medical University)H-Index: 34
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ObjectiveChondrospheres represent a variant of tissue spheroids biofabricated from chondrocytes. They are already being used in clinical trials for cartilage repair; however, their biomechanical pr...
3 CitationsSource
#1V. A. ParfenovH-Index: 5
Last. Vladimir MironovH-Index: 34
view all 18 authors...
: Tissue spheroids have been proposed as building blocks in 3D biofabrication. Conventional magnetic force-driven 2D patterning of tissue spheroids requires prior cell labeling by magnetic nanoparticles, meanwhile a label-free approach for 3D magnetic levitational assembly has been introduced. Here we present first time report on rapid assembly of 3D tissue construct using scaffold-free, nozzle-free and label-free magnetic levitation of tissue spheroids. Chondrospheres of standard size, shape an...
5 CitationsSource