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Lorenzo Alibardi
University of Bologna
81Publications
25H-index
2,070Citations
Publications 81
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#1Lorenzo Alibardi (UNIBO: University of Bologna)H-Index: 27
Abstract After limb amputation in lizards no regeneration occurs following massive inflammatory reaction. Light immunocytochemistry for CD68 and ultrastructural observations show that numerous macrophages persist for over 18 days post-amputation in the limb and fibroblasts producing high levels of collagen are present underneath a differentiating wound epidermis. Injections of 5BrdU for 1 week in normal lizards followed by a 4 weeks chase period indicate that most Long Retention Cells are presen...
#1Matteo Calvaresi (UNIBO: University of Bologna)H-Index: 20
#2Leopold Eckhart (Medical University of Vienna)H-Index: 29
Last.Lorenzo Alibardi (UNIBO: University of Bologna)H-Index: 25
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Abstract The hard corneous material of avian and reptilian scales, claws, beak and feathers is mainly derived from the presence of proteins formerly known as beta-keratins but now termed Corneous beta-proteins of sauropsids to distinguish them from keratins, which are members of the intermediate filament protein family. The modeling of the conserved 34 amino acid residues long central beta-sheet region of Corneous beta-proteins using an ab initio protein folding and structure prediction algorith...
#1Bettina Strasser (Medical University of Vienna)H-Index: 6
#2Veronika Mlitz (Medical University of Vienna)H-Index: 16
Last.Leopold Eckhart (Medical University of Vienna)H-Index: 8
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The evolution of amniotes has involved major molecular innovations in the epidermis. In particular, distinct structural proteins that undergo covalent cross-linking during cornification of keratinocytes facilitate the formation of mechanically resilient superficial cell layers and help to limit water loss to the environment. Special modes of cornification generate amniote-specific skin appendages such as claws, feathers, and hair. In mammals, many protein substrates of cornification are encoded ...
The adaptation of the epidermis in amniote vertebrates to life on land took place by a drastic change from an embryonic epidermis made of two-four periderm layers to a terrestrial-proof epidermis. This transition occurred by the increase in types and number of specialized corneous proteins coded by genes of the Epidermal Differentiation Complex. The prevalent types of corneous proteins produced in the reptilian epidermis contain a beta-sheet region of high amino acid homology which allows their ...
Abstract The present review summarizes biochemical, histochemical and immunocytochemical aspects of the process of tissue regeneration in lizards, non-mammalian amniotes with high regenerative power. The amputated tail initially mobilizes the glycogen and lipid reserves during wound healing. In the following stage of formation of the regenerative blastema tissue remodeling produces a typical embryonic tissue, initially increasing the amount of water and glycosaminoglycans such as jaluronate, whi...
#1Lorenzo Alibardi (UNIBO: University of Bologna)H-Index: 25
Knowledge of beta-protein (beta-keratin) sequences in Anolis carolinensis facilitates the localization of specific sites in the skin of this lizard. The epidermal distribution of two new beta-proteins (beta-keratins), HgGC8 and HgG13, has been analyzed by Western blotting, light and ultrastructural immunocytochemistry. HgGC8 includes 16 kDa members of the glycine-cysteine medium-rich subfamily and is mainly expressed in the beta-layer of adhesive setae but not in the setae. HgGC8 is absent in ot...
#1Lorenzo Alibardi (UNIBO: University of Bologna)H-Index: 25
#2Masaru Tsuchiya (Kao Corporation)H-Index: 3
Last.Bernd NöckerH-Index: 2
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Abstract Unlike the superficial part of the corneous layer of the epidermis ( Stratum corneum ) where desmosomes are degraded and corneocytes flake away, the trichocytes in the hair remain attached to each other after cornification. The permanence and fine localization of cell junctions, in particular of desmosomal proteins in the cornifying and mature human hair, is not known. The present electron microscope immunolocalization study indicates that two protein markers for desmosomes such as desm...
#1Lorenzo Alibardi (UNIBO: University of Bologna)H-Index: 25
Abstract The epidermis of soft-shelled and hard-shelled turtles has been compared to determine the origin of the different cornification. Immunolocalization of acidic alpha-keratin (AK2) of 45–50 kDa in tonofilaments of the epidermis in Apalone spinifera and absence in the corneous layer where desquamating corneocytes are present supports the biochemical data. Corneocytes shows a weak to absent immunolabeling for beta-proteins (formerly beta-keratins) of 14–16 kDa while sparse immunolabeled corn...
#1Lorenzo Alibardi (UNIBO: University of Bologna)H-Index: 25
Abstract The previous identification of 21 proteins in the digital setae transcriptome of Gekko gecko , 2 alpha-keratins of 52–53 kDa and 19 beta-proteins (beta-keratins) of 10–21 kDa, has indicated that most of setal corneous proteins are cysteine-rich. The production of specific antibodies for two of the main beta-protein subfamilies expressed in gecko setae has allowed the ultrastructural localization of two beta-proteins indicated as Ge-cprp-9 (cysteine-rich) and Ge-gprp-6 (glycine-rich). On...
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