Denis Groleau
Molecular biologyInteinPeptideOdorrana grahamiBiochemistry
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Publications 3
#1Yong-Gang Xie (ZJU: Zhejiang University)H-Index: 5
#2Feifei HanH-Index: 9
Last. Yizhen WangH-Index: 27
view all 8 authors...
OG2 is a modified antimicrobial peptide, that is, derived from the frog peptide Palustrin-OG1. It has high antimicrobial activity and low cytotoxicity, and it is therefore promising as a therapeutic agent. Both prokaryotic (Escherichia coli) and eukaryotic (Pichia pastoris) production host systems were used to produce OG2 in our previous study; however, it was difficult to achieve high expression yields and efficient purification. In this study, we achieved high-yield OG2 expression using the in...
10 CitationsSource
#1Yong-Gang Xie (ZJU: Zhejiang University)H-Index: 5
#2Yifan LiuH-Index: 6
Last. Yizhen WangH-Index: 27
view all 8 authors...
Palustrin-OG1 (OG1) is a host defense peptide isolated from the frog Odorrana grahami. In this study, we analyzed the chemical properties, antimicrobial activities and cytotoxicities of OG1 and its derivatives to identify the most promising peptide as an antimicrobial agent. By increasing the net positive charge, amphipathicity and decreasing the mean hydrophobicity of OG1, the derivative named as OG2 exerted higher antimicrobial activity against bacteria but lower cytotoxicity against both porc...
3 CitationsSource
#1Yong-Gang XieH-Index: 5
#2Chao LuanH-Index: 8
Last. Yizhen WangH-Index: 27
view all 8 authors...
OG2 is a modified antimicrobial peptide of Palustrin-OG1 (OG1), which is derived from Odorrana grahami frog. OG2 has shown much higher selective antimicrobial activity and lower hemolytic activity than OG1, indicating OG2 may be a promising antimicrobial agent. In this study, we investigated three fusion partners, including thioredoxin, Mxe GyrA intein, and small ubiquitin-like modifier (SUMO), each fused with OG2, and examined their effects on the expression level and solubility of OG2 in Esche...
18 CitationsSource