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Xiaowei Yang
University of Kentucky
4Publications
4H-index
76Citations
Publications 4
Newest
#1Xiaowei Yang (UK: University of Kentucky)H-Index: 4
#2Huipeng Pan (SCAU: South China Agricultural University)H-Index: 22
Last.Xuguo Zhou (UK: University of Kentucky)H-Index: 34
view all 4 authors...
Harmonia axyridis is a voracious predator, a biological control agent, and one of the world most invasive insect species. The advent of next-generation sequencing platforms has propelled entomological research into the genomics and post-genomics era. Real-time quantitative PCR (RT-qPCR), a primary tool for gene expression analysis, is a core technique governs the genomic research. The selection of internal reference genes, however, can significantly impact the interpretation of RT-qPCR results. ...
8 CitationsSource
#1Huipeng Pan (SCAU: South China Agricultural University)H-Index: 22
#2Xiaowei Yang (UK: University of Kentucky)H-Index: 4
Last.Xuguo Zhou (UK: University of Kentucky)H-Index: 34
view all 6 authors...
By suppressing the expression of genes with essential biological functions, in planta RNAi can negatively affect the development and survival of target pests. As a part of a concerted effort to assess the risks of RNAi transgenic crops on non-target organisms, we developed an in vivo toxicity assay to examine the impacts of ingested dsRNAs incurred to the monarch butterfly, Danaus plexippus (L.), an iconic eco-indicator in North America. To create the worst case scenario, the full-length v-ATPas...
6 CitationsSource
#1Huipeng Pan (UK: University of Kentucky)H-Index: 22
#2Xiaowei Yang (UK: University of Kentucky)H-Index: 4
Last.Xuguo Zhou (UK: University of Kentucky)H-Index: 34
view all 6 authors...
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a powerful technique to quantify gene expression. To facilitate gene expression study and obtain accurate results, normalization relative to stably expressed reference genes is crucial. The monarch butterfly, Danaus plexippus (L.), is one of the most recognized insect species for its spectacular annual migration across North America. Besides its great voyages, D. plexippus has drawn attention to its role as a bio-indicator...
21 CitationsSource
#1Huipeng Pan (UK: University of Kentucky)H-Index: 22
#2Xiaowei Yang (UK: University of Kentucky)H-Index: 4
Last.Xuguo Zhou (UK: University of Kentucky)H-Index: 34
view all 4 authors...
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable, rapid, and reproducible technique for measuring and evaluating changes in gene expression. To facilitate gene expression studies and obtain more accurate RT-qPCR data, normalization relative to stable reference genes is required. In this study, expression profiles of seven candidate reference genes, including β-actin (Actin), elongation factor 1 α (EF1A), glyceralde hyde-3-phosphate dehydro-genase (GAPDH), cycl...
41 CitationsSource
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