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Barbara Giabbai
Elettra Sincrotrone Trieste
12Publications
2H-index
28Citations
Publications 13
Newest
#1Gianluca Veggiani (UNG: University of Nova Gorica)
#2Barbara Giabbai (Elettra Sincrotrone Trieste)H-Index: 2
Last.Ario de Marco (UNG: University of Nova Gorica)H-Index: 15
view all 8 authors...
Abstract Recombinant antibodies can be expressed as fusion constructs in combination with tags which simplify their engineering into reliable and homogeneous immunoreagents by allowing site-specific, 1:1 functionalization. Several tags and corresponding reagents for recombinant protein derivatization have been proposed but benchmarking surveys for the evaluation of their effect on the characteristics of recombinant antibodies have not been reported. In this work we evaluated the impact on expres...
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The formation of multienzymatic complexes allows for the fine tuning of many aspects of enzymatic functions, such as efficiency, localization, stability, and moonlighting. Here, we investigated, in solution, the structure of bacterial cysteine synthase (CS) complex. CS is formed by serine acetyltransferase (CysE) and O-acetylserine sulfhydrylase isozyme A (CysK), the enzymes that catalyze the last two steps of cysteine biosynthesis in bacteria. CysK and CysE have been proposed as potential targe...
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#1V. PirettiH-Index: 1
#2Barbara GiabbaiH-Index: 2
Last.Andrea CavalliH-Index: 48
view all 10 authors...
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#1V. PirettiH-Index: 1
#2Barbara GiabbaiH-Index: 2
Last.Andrea CavalliH-Index: 48
view all 10 authors...
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#1D. Gobbo (UNIBO: University of Bologna)H-Index: 1
#2V. Piretti (UNIBO: University of Bologna)H-Index: 1
Last.Andrea Cavalli (UNIBO: University of Bologna)H-Index: 48
view all 10 authors...
It is widely accepted that drug–target association and dissociation rates directly affect drug efficacy and safety. To rationally optimize drug binding kinetics, one must know the atomic arrangement of the protein–ligand complex during the binding/unbinding process in order to detect stable and metastable states. Whereas experimental approaches can determine kinetic constants with fairly good accuracy, computational approaches based on molecular dynamics (MD) simulations can deliver the atomisti...
1 CitationsSource
#1Samanta Raboni (University of Parma)
#2Svetlana V. Revtovich (EIMB: Engelhardt Institute of Molecular Biology)H-Index: 7
Last.Andrea Mozzarelli (University of Parma)H-Index: 3
view all 18 authors...
Abstract Methionine deprivation of cancer cells, which are deficient in methionine biosynthesis, has been envisioned as a therapeutic strategy to reduce cancer cell viability. Methionine γ-lyase (MGL), an enzyme that degrades methionine, has been exploited to selectively remove the amino acid from cancer cell environment. In order to increase MGL catalytic activity, we performed sequence and structure conservation analysis of MGLs from various microorganisms. Whereas most of the residues in the ...
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Baculovirus-insect cell expression system has become one of the most widely used eukaryotic expression systems for heterologous protein production in many laboratories. The availability of robust insect cell lines, serum-free media, a range of vectors and commercially-packaged kits have supported the demand for maximizing the exploitation of the baculovirus-insect cell expression system. Naturally, this resulted in varied strategies adopted by different laboratories to optimize protein productio...
3 CitationsSource
Methionine deprivation of cancer cells, which are deficient in methionine biosynthesis, has been envisioned as a therapeutic strategy to reduce cancer cell viability. Methionine γ-lyase (MGL), an enzyme that degrades methionine, has been exploited to selectively remove the amino acid from cancer cell environment. In order to increase MGL catalytic activity, we performed sequence and structure conservation analysis of MGLs from various microorganisms. Whereas most of the residues in the active si...
#1Elena Campaner (UniTS: University of Trieste)H-Index: 3
#2Alessandra Rustighi (AREA Science Park)H-Index: 17
Last.Giannino Del Sal (UniTS: University of Trieste)H-Index: 46
view all 24 authors...
PIN1 is a promising therapeutic target for cancer treatment. In this study, the authors identify a covalent inhibitor of PIN1 with anti-tumour and anti-metastatic properties thanks to PIN1 inactivation and to the release, after binding to PIN1, of a quinone-mimicking compound that elicits reactive oxygen generation and causes DNA damage.
24 CitationsSource
#1Sabrina Pricl (UniTS: University of Trieste)H-Index: 43
#2Erik Laurini (UniTS: University of Trieste)H-Index: 22
Last.Alfonso Quintas-Cardama (Novartis)H-Index: 1
view all 9 authors...
Background Our recent work revealed that some chronic myeloid leukemia (CML) patients failing imatinib therapy carry the BCR-ABL1 V304D mutation, and is associated with poor clinical outcomes. BCR-ABL1 V304D variant has rarely been described in clinical specimens, and its location within the BCR-ABL1 Kinase Domain (KD) appears to be in an area of the protein not involved in the direct binding with tyrosine kinase inhibitors (TKIs) such as Imatinib and Ponatinib. The present study integrates comp...
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