Yong Wang
Third Military Medical University
Publications 23
#1Dong Niu (ZJU: Zhejiang University)H-Index: 8
#2Hong-Jiang Wei (Yunnan Agricultural University)H-Index: 6
Last.Luhan YangH-Index: 16
view all 22 authors...
Xenotransplantation is a promising strategy to alleviate the shortage of organs for human transplantation. In addition to the concerns about pig-to-human immunological compatibility, the risk of cross-species transmission of porcine endogenous retroviruses (PERVs) has impeded the clinical application of this approach. We previously demonstrated the feasibility of inactivating PERV activity in an immortalized pig cell line. We now confirm that PERVs infect human cells, and we observe the horizont...
159 CitationsSource
#1Fangxiang Mu (Third Military Medical University)H-Index: 1
#2Hong Wu (Third Military Medical University)H-Index: 1
Last.Yong Wang (Third Military Medical University)H-Index: 11
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BACKGROUND There have been few studies on the value of various antibody combinations in rheumatoid arthritis (RA) diagnosis, and a lack of studies with large sample sizes, especially in the Chinese population. This study retrospectively evaluated the diagnostic value of a combined assay of five auto-antibodies [anti-cyclic citrullinated peptide (anti-CCP), anti-keratin (AKA), anti-RA 33, glucose-6-phosphate isomerase (GPI), and rheumatoid factor (RF)] for RA. MATERIAL AND METHODS Data were obtai...
1 CitationsSource
#1Kai Liu (Capital Medical University)H-Index: 7
#2Jian Mao (NKU: Nankai University)H-Index: 5
Last.Lin Liu (NKU: Nankai University)H-Index: 44
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Pigs are proposed to be suitable large animal models for test of the efficacy and safety of induced pluripotent stem cells (iPSCs) for stem cell therapy, but authentic pig ES/iPS cell lines with germline competence are rarely produced. The pathways or signaling underlying the defective competent pig iPSCs remain poorly understood. By improving induction conditions using various small chemicals, we generated pig iPSCs that exhibited high pluripotency and differentiation capacity that can contribu...
2 CitationsSource
#1Yong WangH-Index: 11
#2Yinan DuH-Index: 4
Last.Hong WeiH-Index: 21
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Donor major histocompatibility complex class I (MHC I) molecules are the main targets of the host immune response after organ allotransplantation. Whether and how MHC I-deficiency of pig donor tissues affects rejection after xenotransplantation has not been assessed. Beta2-microglobulin (B2M) is indispensable for the assembly of MHC I receptors and therefore provides an effective target to disrupt cell surface MHC I expression. Here, we report the one-step generation of mutant pigs with targeted...
8 CitationsSource
#1Xiaoyang Zhou (Third Military Medical University)H-Index: 8
#2Lulu Wang (Third Military Medical University)H-Index: 6
Last.Hong Wei (Third Military Medical University)H-Index: 21
view all 12 authors...
Precise genetic mutation of model animals is highly valuable for functional investigation of human mutations. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9)-induced homology-directed repair (HDR) is usually used for precise genetic mutation, being limited by the relatively low efficiency compared with that of non-homologous end joining (NHEJ). Although inhibition of NHEJ was shown to enhance HDR-derived mutation, in this work, without inhibition of ...
35 CitationsSource
#1Jin PengH-Index: 1
#2Yong WangH-Index: 11
Last.Pumin ZhangH-Index: 36
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Precise genome modification in large domesticated animals is desirable under many circumstances. In the past it is only possible through lengthy and burdensome cloning procedures. Here we attempted to achieve that goal through the use of the newest genome-modifying tool CRISPR/Cas9. We set out to knockin human albumin cDNA into pig Alb locus for the production of recombinant human serum albumin (rHSA). HSA is a widely used human blood product and is in high demand. We show that homologous recomb...
41 CitationsSource
#1Yong WangH-Index: 11
#2Yinan DuH-Index: 4
Last.Hong WeiH-Index: 21
view all 14 authors...
Co-injection of zygotes with Cas9 mRNA and sgRNA has been proven to be an efficient gene-editing strategy for genome modification of different species. Genetic engineering in pigs holds a great promise in biomedical research. By co-injection of one-cell stage embryos with Cas9 mRNA and Npc1l1 sgRNA, we achieved precise Npc1l1 targeting in Chinese Bama miniature pigs at the efficiency as high as 100%. Meanwhile, we carefully analyzed the Npc1l1 sgRNA:Cas9-mediated on- and off-target mutations in ...
64 CitationsSource
#1Yong Wang (Third Military Medical University)H-Index: 11
#2Huaqiang Yang (CAS: Chinese Academy of Sciences)H-Index: 14
Last.Liang Xue Lai (CAS: Chinese Academy of Sciences)H-Index: 1
view all 16 authors...
Porcine skin is frequently used as a substitute of human skin to cover large wounds in clinic practice of wound care. In our previous work, we found that transgenic expression of human cytoxicT-lymphocyte associated antigen4-immunoglobulin (hCTLA4Ig) in murine skin graft remarkably prolonged its survival in xenogeneic wounds without extensive immunosuppression in recipients, suggesting that transgenic hCTLA4Ig expression in skin graft may be an effective and safe method to prolong xenogeneic ski...
13 CitationsSource
#1Yong Wang (Third Military Medical University)H-Index: 11
#2Daizhi Peng (Third Military Medical University)H-Index: 7
Last.Bing HeH-Index: 1
view all 5 authors...
Background Inhibition of CC chemokine ligand 20 (CCL20), which is expressed by human keratinocytes after proinflammatory cytokine stimulation, may reduce migration of recipient Langerhans cells into tissue-engineered allogeneic skin grafts and minimize immune rejection by the recipient. Here, we screened CCL20 gene knockout clones in the human immortalized skin keratinocyte line HaCaT and tested multiple transfection methods for optimal efficiency.
3 CitationsSource
#1Yong Wang (Third Military Medical University)H-Index: 11
#2Xiaoyang Zhou (Third Military Medical University)H-Index: 8
Last.Hong Wei (Third Military Medical University)H-Index: 21
view all 8 authors...
The meganuclease I-SceI has been effectively used to facilitate transgenesis in fish eggs for nearly a decade. I-SceI-mediated transgenesis is simply via embryo cytoplasmic microinjection and only involves plasmid vectors containing I-SceI recognition sequences, therefore regarding the transgenesis process and application of resulted transgenic organisms, I-SceI-mediated transgenesis is of minimal bio-safety concerns. However, currently no transgenic mammals derived from I-SceI-mediated transgen...
11 CitationsSource