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Patricia Bordes
University of Toulouse
10Publications
4H-index
131Citations
Publications 10
Newest
#1Valérie Guillet (University of Toulouse)H-Index: 5
#2Patricia Bordes (University of Toulouse)H-Index: 4
Last.Lionel Mourey (University of Toulouse)H-Index: 7
view all 13 authors...
SecB chaperones assist protein export by binding both unfolded proteins and the SecA motor. Certain SecB homologs can also control toxin-antitoxin (TA) systems known to modulate bacterial growth in response to stress. In such TA-chaperone (TAC) systems, SecB assists the folding and prevents degradation of the antitoxin, thus facilitating toxin inhibition. Chaperone dependency is conferred by a C-terminal extension in the antitoxin known as chaperone addiction (ChAD) sequence, which makes the ant...
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#1Valérie Guillet (University of Toulouse)H-Index: 5
#2Patricia Bordes (University of Toulouse)H-Index: 4
Last.Lionel Mourey (University of Toulouse)H-Index: 7
view all 13 authors...
The original version of this Article contained errors in Figures 1 and 4. In Fig. 1b, the Mtb-SecBTA sequence was displayed incorrectly. In the inset panel within Fig. 4c, the y-axis of the graph incorrectly read (Q.Rg)2 × I(Q)//(0), and should have read (Q.Rg)2 × I(Q)/I(0). These errors have been corrected in both the PDF and HTML versions of the Article.
1 CitationsSource
#1Hatice Akarsu (Swiss Institute of Bioinformatics)H-Index: 1
#2Patricia Bordes (University of Toulouse)H-Index: 4
Last.Laurent Falquet (Swiss Institute of Bioinformatics)H-Index: 27
view all 6 authors...
Bacterial Toxin-Antitoxin systems (TAS) are involved in key biological functions including plasmid maintenance, defense against phages, persistence and virulence. They are found in nearly all phyla and classified into 6 different types based on the mode of inactivation of the toxin, with the type II TAS being the best characterized so far. We have herein developed a new in silico discovery pipeline named TASmania, which mines the >41K assemblies of the EnsemblBacteria database for known and unch...
2 CitationsSource
#1Pauline Texier (University of Toulouse)
#2Michèle Coddeville (University of Toulouse)H-Index: 8
Last.Pierre Genevaux (University of Toulouse)H-Index: 26
view all 4 authors...
A direct method to study essential genes is to construct conditional knock-down mutants by replacement of their native promoter by an inducible one. In Mycobacteria, replacement of an essential gene promoter with an anhydrotetracycline inducible one was successfully used but required a multi-step approach. In this work, we describe a gene cassette for the engineering of a conditional knock-down mutant, which allows the one-step targeted replacement of mycobacterial promoters by an anhydrotetracy...
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#1Ambre Julie Sala (University of Toulouse)H-Index: 4
#2Patricia Bordes (University of Toulouse)H-Index: 4
Last.Pierre Genevaux (University of Toulouse)H-Index: 26
view all 10 authors...
SecB chaperones assist protein export in bacteria. However, certain SecB family members have diverged to become specialized toward the control of toxin-antitoxin (TA) systems known to promote bacterial adaptation to stress and persistence. In such tripartite TA-chaperone (TAC) systems, the chaperone was shown to assist folding and to prevent degradation of its cognate antitoxin, thus facilitating inhibition of the toxin. Here, we used both the export chaperone SecB of Escherichia coli and the tr...
4 CitationsSource
#1Patricia Bordes (University of Toulouse)H-Index: 4
#2Pierre Genevaux (University of Toulouse)H-Index: 26
Source
#1Patricia Bordes (University of Toulouse)H-Index: 4
#2Laure Lavatine (University of Toulouse)H-Index: 1
Last.Claude Gutierrez (University of Toulouse)H-Index: 9
view all 11 authors...
Bacteria often encounter diverse and rapidly changing environments. To overcome harmful situations, they must be capable of sensing external changes and transmitting this information across biological membranes into the cell, which results in the appropriate redirection of gene expression to prevent or repair cellular damages caused by stress. Extracytoplasmic function (ECF) σ factors provide one common means of bacterial signal transduction to regulate gene expression in response to various ext...
20 CitationsSource
#1Patricia Bordes (University of Toulouse)H-Index: 4
#2Annie Conter (University of Toulouse)H-Index: 5
Last.Claude Gutierrez (University of Toulouse)H-Index: 9
view all 6 authors...
The sigmaS subunit of RNA polymerase is a key regulator of Escherichia coli transcription in stress conditions. sigmaS accumulates in cells subjected to stresses such as an osmotic upshift or the entry into stationary phase. We show here that, at elevated osmolarity, sigmaS accumulates long before the beginning of the sigmaS-dependent induction of osmEp, one of its target promoters. A combination of in vivo and in vitro evidence indicates that a high level of DNA negative supercoiling inhibits t...
67 CitationsSource
#1Patricia Bordes (University of Toulouse)H-Index: 4
#2Jean Bouvier (University of Toulouse)H-Index: 4
Last.Claude Gutierrez (University of Toulouse)H-Index: 9
view all 5 authors...
Transcription of the gene osmE of Escherichia coli is osmotically inducible and regulated by the growth phase. Expression of osmE is directed by a single promoter, osmE p , which is recognized by Eσ70 and Eσs, two forms of RNA polymerase using, respectively, the sigma factors σ70 and σs. Eσs transcribes osmE p during entry into stationary phase. Eσ70 is responsible for osmotic induction of osmE p during the exponential growth phase. In a search for proteins that can modulate osmE p expression in...
9 CitationsSource
A Lactococcus lactis glycine-betaine transport system was identified by functional complementation of an Escherichia coli proP proU mutant with a gene library from L. lactis sbsp. cremoris. The cloned locus forms an operon highly homologous to opuA, encoding a glycine-betaine uptake system of Bacillus subtilis. Disruption of opuA in L. lactis abolished protection by glycine-betaine against elevated osmolarity. OpuA belongs to the so-called “ABC transporters” family, which comprise an extracellul...
28 Citations
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