Xingxu Huang
ShanghaiTech University
Publications 50
#1Guanglei Li (Guangzhou Medical University)H-Index: 4
#2Xinyi Liu (JNU: Jinan University)
Last.Xingxu Huang (CAS: Chinese Academy of Sciences)H-Index: 14
view all 11 authors...
Base editing systems show their power in modeling and correcting the pathogenic mutations of genetic diseases. Previous studies have already demonstrated the editing efficiency of BE3-mediated C-to-T conversion in human embryos. However, the precision and efficiency of a recently developed adenine base editor (ABE), which converts A-to-G editing in human embryos, remain to be addressed. Here we selected reported pathogenic mutations to characterize the ABE in human tripronuclear embryos. We foun...
#1Shuzhen Zhang (Third Military Medical University)
#2Songjie Feng (ShanghaiTech University)
Last.Jieping Chen (Third Military Medical University)H-Index: 1
view all 5 authors...
#1Guanwei Li (NWAFU: Northwest A&F University)H-Index: 1
#2Shiwei Zhou (NWAFU: Northwest A&F University)H-Index: 4
Last.Qiang Ding (NWAFU: Northwest A&F University)H-Index: 2
view all 23 authors...
#1Jianye Wang (SJTU: Shanghai Jiao Tong University)
#2Bian Hu (ShanghaiTech University)
Last.Xingxu Huang (ShanghaiTech University)H-Index: 14
view all 12 authors...
Endoplasmic reticulum (ER) stress-associated cell death is prevalent in various liver diseases. However, the determinant mechanism how hepatocytes survive unresolved stress was still unclear. Interleukin-24 (IL-24) was previously found to promote ER stress-mediated cell death, and yet its expression and function in the liver remained elusive. Here we identified an anti-apoptotic role of IL-24, which transiently accumulated within ER-stressed hepatocytes in a X-box binding protein 1 (XBP1)-depend...
#1Shisheng HuangH-Index: 3
#2Zhaodi LiaoH-Index: 1
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RNA splicing is related to many human diseases; however, lack of efficient genetic approaches to modulate splicing has prevented us from dissecting their functions in human diseases. Recently developed base editors (BEs) offer a new strategy to modulate RNA splicing by converting conservative splice sites, but it is limited by the editing precision and scope. To overcome the limitations of currently available BE-based tools, we combined SpCas9-NG with ABE-max to generate a new BE, ABEmax-NG. We ...
#1Binbin Ma (SJTU: Shanghai Jiao Tong University)H-Index: 1
#2Tl Lee (CUHK: The Chinese University of Hong Kong)H-Index: 32
Last.Chen Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 1
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#1Bian Hu (National Resource Center)
#2Yan Zou (ShanghaiTech University)
Last.Xuekai Zhu (ShanghaiTech University)
view all 8 authors...
CRISPR/Cas9-mediated programmed cell death protein 1 (PD-1) disruption in chimeric antigen receptor (CAR) T cells could be an appealing choice to improve the therapeutic efficacy of CAR T cells in an immunosuppressive tumor microenvironment. In most of the reported cases, Cas9 was delivered into T cells by way of electroporation with RNA or protein. However, transient expression of Cas9 by transfection with a plasmid encoding its gene is apparently simpler, as it avoids the steps of in vitro tra...
#1Bin Lu (WMU: Wenzhou Medical College)H-Index: 12
#2Fugen Shangguan (WMU: Wenzhou Medical College)
Last.Tongke Chen (WMU: Wenzhou Medical College)
view all 21 authors...
Protein quality control is pivotal to cellular homeostasis and integrity of cardiomyocytes for maintenance of normal heart function. The unfolded protein response (UPR) is an adaptive process to modulate protein quality control in the endoplasmic reticulum (ER) and mitochondria, and is accordingly termed UPRER and UPRmt, respectively. Lon protease (LonP1) is a highly conserved mitochondrial protease to modulate UPRmt, which is involved in regulating metabolism, mitophagy, and stress response. Ho...