Xingxu Huang
ShanghaiTech University
Publications 44
Published on Jun 1, 2019in Molecular therapy. Nucleic acids5.92
Guanglei Li4
Estimated H-index: 4
(Guangzhou Medical University),
Xinyi Liu (JNU: Jinan University)+ 8 AuthorsXingxu Huang14
Estimated H-index: 14
(CAS: Chinese Academy of Sciences)
Base editing systems show their power in modeling and correcting the pathogenic mutations of genetic diseases. Previous studies have already demonstrated the editing efficiency of BE3-mediated C-to-T conversion in human embryos. However, the precision and efficiency of a recently developed adenine base editor (ABE), which converts A-to-G editing in human embryos, remain to be addressed. Here we selected reported pathogenic mutations to characterize the ABE in human tripronuclear embryos. We foun...
Published on Jul 5, 2019in FEBS Journal4.74
Guanwei Li1
Estimated H-index: 1
(NWAFU: Northwest A&F University),
Shiwei Zhou4
Estimated H-index: 4
(NWAFU: Northwest A&F University)
+ 20 AuthorsQiang Ding2
Estimated H-index: 2
(NWAFU: Northwest A&F University)
Published on Jun 4, 2019in bioRxiv
Jianye Wang (SJTU: Shanghai Jiao Tong University), Bian Hu (ShanghaiTech University)+ 9 AuthorsXingxu Huang14
Estimated H-index: 14
(ShanghaiTech University)
Endoplasmic reticulum (ER) stress-associated cell death is prevalent in various liver diseases. However, the determinant mechanism how hepatocytes survive unresolved stress was still unclear. Interleukin-24 (IL-24) was previously found to promote ER stress-mediated cell death, and yet its expression and function in the liver remained elusive. Here we identified an anti-apoptotic role of IL-24, which transiently accumulated within ER-stressed hepatocytes in a X-box binding protein 1 (XBP1)-depend...
Published on May 1, 2019in iScience
Shisheng Huang3
Estimated H-index: 3
Zhaodi Liao1
Estimated H-index: 1
+ 9 AuthorsXiajun Li
RNA splicing is related to many human diseases; however, lack of efficient genetic approaches to modulate splicing has prevented us from dissecting their functions in human diseases. Recently developed base editors (BEs) offer a new strategy to modulate RNA splicing by converting conservative splice sites, but it is limited by the editing precision and scope. To overcome the limitations of currently available BE-based tools, we combined SpCas9-NG with ABE-max to generate a new BE, ABEmax-NG. We ...
Published on Feb 1, 2019in Cell Research17.85
Jianan Li8
Estimated H-index: 8
(CAS: Chinese Academy of Sciences),
Zhen Liu11
Estimated H-index: 11
(CAS: Chinese Academy of Sciences)
+ 11 AuthorsHanhui Ma (ShanghaiTech University)
Published on Jan 1, 2019in Cell Reports7.82
Guang Yang9
Estimated H-index: 9
(CAS: Chinese Academy of Sciences),
Changyang Zhou3
Estimated H-index: 3
(CAS: Chinese Academy of Sciences)
+ 12 AuthorsWenqin Ying7
Estimated H-index: 7
(CAS: Chinese Academy of Sciences)
The coactivator-associated arginine methyltransferase CARM1 catalyzes the methylation of histone H3 arginine 17/26 (H3R17/26me) and non-histone proteins at arginine residues to regulate gene transactivation through profiling or Carm1 overexpression assays. However, the direct relationship between H3R17/26me and its causal role in mouse embryo development remains largely unclear. Here, we use rAPOBEC1-XTEN-Cas9n-UGI (BE3) to efficiently introduce a point mutation (R17H) at multiple Hist1/2H3 loci...
Published on Dec 1, 2018in Journal of Translational Medicine4.10
Honghao Yu5
Estimated H-index: 5
(ShanghaiTech University),
Weihu Long1
Estimated H-index: 1
(Yunnan Agricultural University)
+ 10 AuthorsBaoyu Jia4
Estimated H-index: 4
(Yunnan Agricultural University)
Background: Laron syndrome is an autosomal disease resulting from mutations in the growth hormone receptor (GHR) gene. The only therapeutic treatment for Laron syndrome is recombinant insulin-like growth factor I (IGF-I), which has been shown to have various side effects. The improved Laron syndrome models are important for better understanding the pathogenesis of the disease and developing corresponding therapeutics. Pigs have become attractive biomedical models for human condition due to simil...
Published on Dec 1, 2018in Scientific Reports4.01
Weihua Jiang3
Estimated H-index: 3
(SJTU: Shanghai Jiao Tong University),
Lili Liu1
Estimated H-index: 1
(SJTU: Shanghai Jiao Tong University)
+ 10 AuthorsXingxu Huang14
Estimated H-index: 14
CRISPR/Cas9 has recently been developed as an efficient genome engineering tool. The rabbit is a suitable animal model for studies of metabolic diseases. In this study, we generated ATP7B site-directed point mutation rabbits to simulate a major mutation type in Asians (p. Arg778Leu) with Wilson disease (WD) by using the CRISPR/Cas9 system combined with single-strand DNA oligonucleotides (ssODNs). The efficiency of the precision point mutation was 52.94% when zygotes were injected 14 hours after ...