Xingxu Huang
ShanghaiTech University
Cas9Molecular biologyGeneticsBiologyCRISPR
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Publications 58
#1Xinjie Wang (ShanghaiTech University)
Last. Xingxu Huang (ShanghaiTech University)H-Index: 26
view all 3 authors...
Ideal methods for detecting pathogens should be sensitive, specific, rapid, cost-effective and instrument-free. Conventional nucleic acid pathogen detection strategies, mostly PCR-based techniques, have various limitations, such as expensive equipment, reagents and skilled performance. Recently, CRISPR/Cas-based methods have burst onto the scene, with the potential to power the pathogen detection field. Here we introduce these unique methods and discuss its hurdles and promises.
#1Shiwei Zhou (NWAFU: Northwest A&F University)H-Index: 8
#2Yige Ding (NWAFU: Northwest A&F University)H-Index: 3
Last. Peter Kalds (NWAFU: Northwest A&F University)H-Index: 2
view all 21 authors...
Base editing has the potential to improve important economic traits in agriculture and can precisely convert single nucleotides in DNA or RNA sequences into minimal double-strand DNA breaks (DSB). Adenine base editors (ABE) have recently emerged as a base editing tool for the conversion of targeted A:T to G:C, but have not yet been used in sheep. ABEmax is one of the latest versions of ABE, which consists of a catalytically-impaired nuclease and a laboratory-evolved DNA-adenosine deaminase. The ...
#1Xinjie Wang (ShanghaiTech University)H-Index: 1
#2Mingtian Zhong (SCNU: South China Normal University)H-Index: 1
Last. Guang Yang (ShanghaiTech University)H-Index: 6
view all 13 authors...
2 CitationsSource
#1Peter Kalds (NWAFU: Northwest A&F University)H-Index: 2
#2Yawei Gao (NWAFU: Northwest A&F University)
Last. Yulin Chen (NWAFU: Northwest A&F University)H-Index: 18
view all 7 authors...
Abstract Genetic modification is a rapidly developing field in which numerous significant breakthroughs have been achieved. Over the last few decades, genetic modification has evolved from insertional transgenesis to gene targeting and editing and, more recently, to base and prime editing using CRISPR-derived systems. Currently, CRISPR-based genome editing systems are showing great potential for generating gene-edited offspring with defined genetic characteristics. Domestic small ruminants (shee...
#1Jianye Wang (SJTU: Shanghai Jiao Tong University)H-Index: 6
#2Bian Hu (ShanghaiTech University)H-Index: 2
Last. Qiang Xia (SJTU: Shanghai Jiao Tong University)H-Index: 24
view all 12 authors...
Endoplasmic reticulum (ER) stress-associated cell death is prevalent in various liver diseases. However, the determinant mechanism how hepatocytes survive unresolved stress was still unclear. Interleukin-24 (IL-24) was previously found to promote ER stress-mediated cell death, and yet its expression and function in the liver remained elusive. Here we identified an antiapoptotic role of IL-24, which transiently accumulated within ER-stressed hepatocytes in a X-box binding protein 1 (XBP1)-depende...
2 CitationsSource
#1Guanwei Li (NWAFU: Northwest A&F University)H-Index: 4
#2Shiwei Zhou (NWAFU: Northwest A&F University)H-Index: 8
Last. X. J. Wang (NWAFU: Northwest A&F University)H-Index: 1
view all 23 authors...
The ability to alter single bases without homology directed repair (HDR) of double-strand breaks provides a potential solution for editing livestock genomes for economic traits, which are often multigenic. Progress toward multiplex editing in large animals has been hampered by the costly inefficiencies of HDR via microinjection of in vitro manipulated embryos. Here, we designed sgRNAs to induce nonsense codons (C-to-T transitions) at four target sites in caprine FGF5, which is a crucial regulato...
4 CitationsSource
#1Zhongming Tan (Nanjing Medical University)H-Index: 8
#2Wei Zhang (Nanjing Medical University)H-Index: 39
Last. B. Sun (Nanjing Medical University)
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Abstract Congenital hepatic arteriovenous fistulae (CHAVF) are direct communications between the hepatic artery and portal vein or hepatic vein. Clinical symptoms of CHAVF depend mainly on the location, duration, and blood flow volume of the fistulae, which are manifested by portal hypertension, hepatic fibrosis, cardiac enlargement, and eventually heart failure. Here we report a female patient aged 54 who was first admitted to our hospital due to recurrent chest tightness and palpitations in Ma...
#1Shiwei Zhou (NWAFU: Northwest A&F University)H-Index: 8
#2Honghao Yu (NWAFU: Northwest A&F University)H-Index: 1
Last. X. J. Wang (NWAFU: Northwest A&F University)H-Index: 1
view all 16 authors...
Since its emergence, the clustered regularly interspaced short palindromic repeat (CRISPR)–CRISPR-associated (Cas) 9 system has been increasingly used to generate animals for economically important traits. However, most CRISPR/Cas9 applications have been focused on non-homologous end joining, which results in base deletions and insertions, leading to a functional knockout of the targeted gene. The Booroola fecundity gene (FecBB) mutation (p.Q249R) in bone morphogenetic protein receptor type 1B (...
9 CitationsSource
#1Chao Li (NWAFU: Northwest A&F University)H-Index: 8
#2Shiwei Zhou (NWAFU: Northwest A&F University)H-Index: 8
Last. X. J. Wang (NWAFU: Northwest A&F University)H-Index: 1
view all 14 authors...
Unintended off-target mutations induced by CRISPR/Cas9 nucleases may result in unwanted consequences, which will impede the efficient applicability of this technology for genetic improvement. We have recently edited the goat genome through CRISPR/Cas9 by targeting MSTN and FGF5, which increased muscle fiber diameter and hair fiber length, respectively. Using family trio-based sequencing that allow better discrimination of variant origins, we herein generated offspring from edited goats, and sequ...
14 CitationsSource
#1Xiao WangH-Index: 2
#1Xiao Wang (CAS: Chinese Academy of Sciences)H-Index: 16
Last. Li Yang (CAS-MPG Partner Institute for Computational Biology)H-Index: 38
view all 10 authors...
Increased efficiency of base editing in methylated DNA using human APOBEC3A as a deaminase.
60 CitationsSource