Xingxu Huang
Chinese Academy of Sciences
Publications 76
Activation of RIPK1 controls TNF-mediated apoptosis, necroptosis and inflammatory pathways1. Cleavage of human and mouse RIPK1 after residues D324 and D325, respectively, by caspase-8 separates the RIPK1 kinase domain from the intermediate and death domains. The D325A mutation in mouse RIPK1 leads to embryonic lethality during mouse development2,3. However, the functional importance of blocking caspase-8-mediated cleavage of RIPK1 on RIPK1 activation in humans is unknown. Here we identify two fa...
Dynamic N6-methyladenosine (m6A) modification was previously identified as a ubiquitous post-transcriptional regulation that affected mRNA homeostasis. However, the m6A-related epitranscriptomic alterations and functions remain elusive in human cancer. Here we aim to identify the profile and outcome of m6A-methylation in hepatocellular carcinoma (HCC). Using liquid chromatography-tandem mass spectrometry and m6A-immunoprecipitation in combination with high-throughput sequencing, we determined th...
1 CitationsSource
Abstract Traditional CRISPR/Cas9-based gene knockouts are generated by introducing DNA double-strand breaks (DSBs), but this may cause excessive DNA damage or cell death. CRISPR-based cytosine base editors (CBEs) and adenine base editors (ABEs) can facilitate C-to-T or A-to-G exchanges, respectively, without DSBs. CBEs have been employed in a gene knockout strategy: CRISPR-STOP or i-STOP changes single nucleotides to induce in-frame stop codons. However, this strategy is not applicable for some ...
AIM To evaluate the diagnostic performance of dual-energy computed tomography (DECT) using the virtual non-calcium (VNCa) technique for the detection of different degrees of vertebral bone marrow oedema. MATERIALS AND METHODS The present prospective study was approved by the institutional ethics committee. Informed consent was obtained from all patients. Twenty patients underwent both DECT and MRI. Vertebral bodies on VNCa images were graded visually by two blinded, independent radiologists usin...
Abstract Site-directed RNA editing (SDRE) is invaluable to basic research and medicine, and has emerged as a new frontier in genome editing. The past few years have witnessed a surge of interest in SDRE, with SDRE tools emerging at a breathtaking pace. However, off-target effects of SDRE remain a tough problem, which constitutes a major hurdle to their clinical applications. Here we discuss the diverse strategies for combating off-target editing, drawing lessons from the published studies as wel...
#1Guanglei Li (Guangzhou Medical University)H-Index: 4
#2Xinyi Liu (JNU: Jinan University)H-Index: 1
Last.Jianqiao Liu (Guangzhou Medical University)H-Index: 2
view all 11 authors...
Base editing systems show their power in modeling and correcting the pathogenic mutations of genetic diseases. Previous studies have already demonstrated the editing efficiency of BE3-mediated C-to-T conversion in human embryos. However, the precision and efficiency of a recently developed adenine base editor (ABE), which converts A-to-G editing in human embryos, remain to be addressed. Here we selected reported pathogenic mutations to characterize the ABE in human tripronuclear embryos. We foun...
1 CitationsSource
#1Xinyi Liu (JNU: Jinan University)H-Index: 1
#2Guanglei Li (Guangzhou Medical University)H-Index: 4
Last.Xingxu Huang (ShanghaiTech University)H-Index: 22
view all 9 authors...
The development of CRISPR/Cas9-mediated base editors (BEs) provided a versatile tool for precise genome editing. The recently developed xCas9-derived base editors (xBEs) that recognize the NG PAM substantially expand the targeting scope in the genome, while their editing efficiency needs to be improved. Here, we described an improved version of xBEs by fusing the BPNLS and Gam to the N terminus of xBEs (BPNLS-Gam-xBE3 and BPNLS-xABE), and this version of base editor displayed higher targeting ef...
1 CitationsSource
#1Shuzhen Zhang (Third Military Medical University)
#2Songjie Feng (ShanghaiTech University)
Last.Jieping Chen (Third Military Medical University)H-Index: 1
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#1Guanwei Li (NWAFU: Northwest A&F University)H-Index: 3
#2Shiwei Zhou (NWAFU: Northwest A&F University)H-Index: 6
Last.Xiaolong Wang (NWAFU: Northwest A&F University)H-Index: 10
view all 23 authors...
The ability to alter single bases without homology directed repair (HDR) of double-strand breaks provides a potential solution for editing livestock genomes for economic traits, which are often multigenic. Progress toward multiplex editing in large animals has been hampered by the costly inefficiencies of HDR via microinjection of in vitro manipulated embryos. Here, we designed sgRNAs to induce nonsense codons (C-to-T transitions) at four target sites in caprine FGF5, which is a crucial regulato...
#1Jiajie HouH-Index: 7
Last.Qiang XiaH-Index: 21
view all 17 authors...