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Changyang Zhou
Chinese Academy of Sciences
GeneChemistryGeneticsBiologyCRISPR
12Publications
5H-index
131Citations
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Publications 14
Newest
#1Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
#2Xinde Hu (CAS: Chinese Academy of Sciences)H-Index: 9
Last. Xiaodong Sun (SJTU: Shanghai Jiao Tong University)H-Index: 1
view all 12 authors...
2 CitationsSource
#1Erwei ZuoH-Index: 6
#2Yidi Sun (CAS: Chinese Academy of Sciences)H-Index: 8
Last. LIYixue (CAS: Chinese Academy of Sciences)H-Index: 57
view all 11 authors...
Base editors hold promise for correcting pathogenic mutations, while substantial single nucleotide variations (SNVs) on both DNA and RNA were generated by cytosine base editors (CBEs). Here we examined possibilities to reduce off-target effects by engineering cytosine deaminases. By screening 24 CBEs harboring various rAPOBEC1 (BE3) or human APOBEC3A (BE3-hA3A) mutations on the ssDNA or RNA binding domain, we found 8 CBE variations could maintain high on-target editing efficiency. Using Genome-w...
1 CitationsSource
#1Jin-Jing Li (Fujian Medical University)H-Index: 2
#2Xiang Lin (Fujian Medical University)H-Index: 3
Last. Wan-Jin Chen (Fujian Medical University)H-Index: 11
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Source
#1Yajing Liu (CAS: Chinese Academy of Sciences)H-Index: 7
#2Jianan Li (CAS: Chinese Academy of Sciences)
Last. Yunbo Qiao (GU: Guangzhou University)H-Index: 1
view all 11 authors...
Abstract Allele-specific DNA methylation is the most important imprinting marker localized to differentially methylated regions (DMRs), and aberrant genomic imprinted DNA methylation is associated with some human diseases, including Prader-Willi syndrome and cancer. Thus, the development of an effective strategy for the precise editing of allele-specific methylated genes is essential for the functional clarification of imprinting elements and the correction of imprinting disorders in human disea...
Source
#1Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
#2Yidi Sun (CAS: Chinese Academy of Sciences)H-Index: 8
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 14 authors...
Recently developed DNA base editing methods enable the direct generation of desired point mutations in genomic DNA without generating any double-strand breaks1–3, but the issue of off-target edits has limited the application of these methods. Although several previous studies have evaluated off-target mutations in genomic DNA4–8, it is now clear that the deaminases that are integral to commonly used DNA base editors often bind to RNA9–13. For example, the cytosine deaminase APOBEC1—which is used...
16 CitationsSource
#1Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 5
#2Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 17 authors...
Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and four-cell embryos results in much higher (up to 13-fold) homozygotic nucleotide substitution efficiency as opposed to MII oocytes or zygotes. Furthermore, as a proof-of-principle study, a point mutation can be ...
Source
#1Guang Yang (CAS: Chinese Academy of Sciences)H-Index: 9
#2Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
Last. Yunbo Qiao (GU: Guangzhou University)H-Index: 1
view all 15 authors...
The coactivator-associated arginine methyltransferase CARM1 catalyzes the methylation of histone H3 arginine 17/26 (H3R17/26me) and non-histone proteins at arginine residues to regulate gene transactivation through profiling or Carm1 overexpression assays. However, the direct relationship between H3R17/26me and its causal role in mouse embryo development remains largely unclear. Here, we use rAPOBEC1-XTEN-Cas9n-UGI (BE3) to efficiently introduce a point mutation (R17H) at multiple Hist1/2H3 loci...
Source
#1He Zhang (CAS: Chinese Academy of Sciences)H-Index: 2
#2Hong Pan (CAS: Chinese Academy of Sciences)H-Index: 2
Last. Zhen Liu (CAS: Chinese Academy of Sciences)H-Index: 48
view all 17 authors...
ABSTRACT In vivo genetic mutation has become a powerful tool for dissecting gene function; however, multi-gene interaction and the compensatory mechanisms involved can make findings from single mutations, at best difficult to interpret, and, at worst, misleading. Hence, it is necessary to establish an efficient way to disrupt multiple genes simultaneously. CRISPR/Cas9-mediated base editing disrupts gene function by converting a protein-coding sequence into a stop codon; this is referred to as CR...
6 CitationsSource
#1Haibo Zhou (CAS: Chinese Academy of Sciences)H-Index: 3
#2J.Q. Liu (CAS: Chinese Academy of Sciences)H-Index: 4
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 22 authors...
Despite rapid progresses in the genome-editing field, in vivo simultaneous overexpression of multiple genes remains challenging. We generated a transgenic mouse using an improved dCas9 system that enables simultaneous and precise in vivo transcriptional activation of multiple genes and long noncoding RNAs in the nervous system. As proof of concept, we were able to use targeted activation of endogenous neurogenic genes in these transgenic mice to directly and efficiently convert astrocytes into f...
44 CitationsSource
#1Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
#2Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 5
Last. Zi-Jiang Chen (SJTU: Shanghai Jiao Tong University)H-Index: 39
view all 12 authors...
25 CitationsSource
12