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Changyang Zhou
Chinese Academy of Sciences
5Publications
3H-index
80Citations
Publications 5
Newest
Published on Dec 1, 2019in Genome Biology14.03
Meiling Zhang2
Estimated H-index: 2
(SJTU: Shanghai Jiao Tong University),
Changyang Zhou3
Estimated H-index: 3
(CAS: Chinese Academy of Sciences)
+ 14 AuthorsNing Yao2
Estimated H-index: 2
(SJTU: Shanghai Jiao Tong University)
Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and four-cell embryos results in much higher (up to 13-fold) homozygotic nucleotide substitution efficiency as opposed to MII oocytes or zygotes. Furthermore, as a proof-of-principle study, a point mutation can be ...
Yajing Liu6
Estimated H-index: 6
(CAS: Chinese Academy of Sciences),
Jianan Li (CAS: Chinese Academy of Sciences)+ -3 AuthorsHui Yang16
Estimated H-index: 16
Abstract Allele-specific DNA methylation is the most important imprinting marker localized to differentially methylated regions (DMRs), and aberrant genomic imprinted DNA methylation is associated with some human diseases, including Prader-Willi syndrome and cancer. Thus, the development of an effective strategy for the precise editing of allele-specific methylated genes is essential for the functional clarification of imprinting elements and the correction of imprinting disorders in human disea...
Published on Jun 10, 2019in Nature43.07
Changyang Zhou3
Estimated H-index: 3
(CAS: Chinese Academy of Sciences),
Yidi Sun7
Estimated H-index: 7
(CAS: Chinese Academy of Sciences)
+ 11 AuthorsRong Zeng40
Estimated H-index: 40
(CAS: Chinese Academy of Sciences)
Recently developed DNA base editing methods enable the direct generation of desired point mutations in genomic DNA without generating any double-strand breaks1–3, but the issue of off-target edits has limited the application of these methods. Although several previous studies have evaluated off-target mutations in genomic DNA4–8, it is now clear that the deaminases that are integral to commonly used DNA base editors often bind to RNA9–13. For example, the cytosine deaminase APOBEC1—which is used...
Published on Jan 1, 2019in Cell Reports7.82
Guang Yang9
Estimated H-index: 9
(CAS: Chinese Academy of Sciences),
Changyang Zhou3
Estimated H-index: 3
(CAS: Chinese Academy of Sciences)
+ 12 AuthorsWenqin Ying7
Estimated H-index: 7
(CAS: Chinese Academy of Sciences)
The coactivator-associated arginine methyltransferase CARM1 catalyzes the methylation of histone H3 arginine 17/26 (H3R17/26me) and non-histone proteins at arginine residues to regulate gene transactivation through profiling or Carm1 overexpression assays. However, the direct relationship between H3R17/26me and its causal role in mouse embryo development remains largely unclear. Here, we use rAPOBEC1-XTEN-Cas9n-UGI (BE3) to efficiently introduce a point mutation (R17H) at multiple Hist1/2H3 loci...
Published on Oct 15, 2018in Development5.76
He Zhang1
Estimated H-index: 1
(CAS: Chinese Academy of Sciences),
Hong Pan1
Estimated H-index: 1
(CAS: Chinese Academy of Sciences)
+ 14 AuthorsGen Li1
Estimated H-index: 1
(SJTU: Shanghai Jiao Tong University)
ABSTRACT In vivo genetic mutation has become a powerful tool for dissecting gene function; however, multi-gene interaction and the compensatory mechanisms involved can make findings from single mutations, at best difficult to interpret, and, at worst, misleading. Hence, it is necessary to establish an efficient way to disrupt multiple genes simultaneously. CRISPR/Cas9-mediated base editing disrupts gene function by converting a protein-coding sequence into a stop codon; this is referred to as CR...
Published on Mar 1, 2018in Nature Neuroscience21.13
Haibo Zhou2
Estimated H-index: 2
(CAS: Chinese Academy of Sciences),
Junlai Liu5
Estimated H-index: 5
(CAS: Chinese Academy of Sciences)
+ 19 AuthorsXiaowen Shen3
Estimated H-index: 3
(CAS: Chinese Academy of Sciences)
Despite rapid progresses in the genome-editing field, in vivo simultaneous overexpression of multiple genes remains challenging. We generated a transgenic mouse using an improved dCas9 system that enables simultaneous and precise in vivo transcriptional activation of multiple genes and long noncoding RNAs in the nervous system. As proof of concept, we were able to use targeted activation of endogenous neurogenic genes in these transgenic mice to directly and efficiently convert astrocytes into f...
Published on Oct 1, 2017in Protein & Cell7.58
Changyang Zhou3
Estimated H-index: 3
(CAS: Chinese Academy of Sciences),
Meiling Zhang2
Estimated H-index: 2
(SJTU: Shanghai Jiao Tong University)
+ 9 AuthorsWeiping Li7
Estimated H-index: 7
(SJTU: Shanghai Jiao Tong University)
Published on Jul 1, 2017in Cell Research17.85
Erwei Zuo3
Estimated H-index: 3
,
Yijun Cai7
Estimated H-index: 7
+ 26 AuthorsWei Wei44
Estimated H-index: 44
One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs
Published on Jan 1, 1996in Chinese Science Bulletin
Cheng-Sen Li24
Estimated H-index: 24
,
Changren Zhou22
Estimated H-index: 22
+ 2 AuthorsQuanjun Zhang7
Estimated H-index: 7
Published in National Science Review13.22
Jin-Jing Li2
Estimated H-index: 2
(Fujian Medical University),
Xiang Lin3
Estimated H-index: 3
(Fujian Medical University)
+ -3 AuthorsLu-Lu Lai1
Estimated H-index: 1
(Fujian Medical University)
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