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Guo-Jun Zhang
Hubei University of Chinese Medicine
Analytical chemistryBiosensorChemistryGrapheneDNA
50Publications
16H-index
820Citations
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Publications 55
Newest
#1Cancan Wang (Hubei University of Chinese Medicine)H-Index: 1
#2Dan Jin (Hubei University of Chinese Medicine)H-Index: 4
Last. Guo-Jun Zhang (Hubei University of Chinese Medicine)H-Index: 16
view all 7 authors...
Abstract Since exosomes carry the genetic information of their parental cells, there is an increasing need for new technologies capable of detecting exosomes with high sensitivity and accuracy. In this study, we propose a dual antibody-modified nanochannel biosensor for rapid, sensitive analysis of exosomes. Two antibodies (anti-CD63 and anti-EpCAM) were simultaneously immobilized on the surface of the porous anodic alumina (PAA) nanochannel to combine with proteins overexpressed on the exosomal...
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#1Li XuH-Index: 6
#1Li Xu (Hubei University of Chinese Medicine)
Last. Chuluo Yang (SZU: Shenzhen University)
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The development of luminescent probes with long excited state lifetimes is essential for time-resolved biological imaging, among which Ir(Ⅲ) complexes are particularly promising. Many efforts have been devoted to increasing the luminescence efficiency of bioprobe based on Ir(Ⅲ) complex in water. A novel and facile design principle of nanoparticles with highly emissive phosphorescence sensitized by thermally activated delayed fluorescence (TADF) polymer for bioimaging was proposed in this work. H...
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Last. Guo-Jun ZhangH-Index: 16
view all 6 authors...
Cancerous microvesicles (MVs), which are heterogeneous membrane-bound nanovesicles shed from the surfaces of cancer cells into the extracellular environment, have been widely recognized as promising "biofingerprints" for various cancers. High-performance identification of cancerous MVs plays a vital role in the early diagnosis of cancer, yet is still technically challenging. Herein, we report a gold nanoparticles (AuNPs)-decorated, dual-aptamer modified reduced graphene oxide (RGO) field-effect ...
1 CitationsSource
#1Ruixue Shi (Hubei University of Chinese Medicine)H-Index: 1
#2Zhigang Hu (Hubei University of Chinese Medicine)H-Index: 6
Last. Fan Yang (Hubei University of Chinese Medicine)H-Index: 25
view all 11 authors...
It remains a technical challenge to accurately identify close species of herbal medicines, especially from adulterants, because of their highly identical phenotypes and chemical compositions. Here, we report a direct, sequencing-free, high-curvature nanostructuring-based electrochemical herb sensor (nanoE-herb sensor) to identify herbal species quickly and accurately using ITS2 barcodes. We engineer a nano-roughened carbon-supported gold nanostructuring array by photolithograph-free, one-step el...
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#1Chenyun WangH-Index: 1
#2Cancan WangH-Index: 1
Last. Guo-Jun ZhangH-Index: 16
view all 8 authors...
In this study, we report a gold nanoparticle (AuNP)-amplified surface acoustic wave (SAW) sensor for exosome detection with high sensitivity. The SAW chip was self-assembled with mercapto acetic acid to generate carboxylic groups via the Au–S bond. Anti-CD63 was then anchored onto the chip by pretreatment with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide,1-hydroxypyrrolidine-2,5-dione (NHS). Due to the existence of a membrane protein, CD63, on the ex...
2 CitationsSource
#1Ding Wu (Hubei University of Chinese Medicine)
#2Hong Zhang (Hubei University of Chinese Medicine)H-Index: 6
Last. Guo-Jun Zhang (Hubei University of Chinese Medicine)H-Index: 16
view all 9 authors...
Unlike other extracellular vesicle (EV) subtypes such as exosomes, the lack of well-defined universal markers on the surface of microvesicles (MVs) has led to difficulty in the detection of the entire MV population. To design a universal MV detection method, we reported highly sensitive electrical detection of MVs using a reduced graphene oxide (RGO)-based field-effect transistor (FET) biosensor by the introduction of a membrane biotinylation strategy in this work. Biotinylated MVs (B-MVs) were ...
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#1L. LiuH-Index: 7
#2Hao LuH-Index: 1
Last. Guo-Jun ZhangH-Index: 16
view all 10 authors...
Exosomal microRNAs are essential in intercellular communications and disease progression, yet it remains challenging to quantify the expression level due to their small size and low abundance in blood. Here, we report a “sandwich” electrochemical exosomal microRNA sensor (SEEmiR) to detect target microRNA with high sensitivity and specificity. In SEEmiR, neutrally charged peptide nucleic acid (PNA) enables kinetically favorable hybridization with the microRNA target relative to negatively charge...
3 CitationsSource
#1Yi Yu (Hubei University of Chinese Medicine)
#2Yu-Tao Li (Hubei University of Chinese Medicine)H-Index: 4
Last. Guo-Jun Zhang (Hubei University of Chinese Medicine)H-Index: 16
view all 9 authors...
Exosomes are small membrane-bound nanovesicles with a size of 50–150 nm which contain many functional biomolecules, such as nucleic acids and proteins. Due to their high homology with parental generation, they are of great significance in clinical diagnosis. At present, the quantitative detection of low concentrations of cancer-derived exosomes present in biofluids is still a great challenge. In this study, we develop an electrical and label-free method to directly detect exosomes with high sens...
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#1Yu-Tao LiH-Index: 7
Last. Guo-Jun ZhangH-Index: 16
view all 8 authors...
Glutamate, one of the most important central excitatory neurotransmitters, plays crucial roles in nerve signal transduction and is implicated in several neurological disorders. However, no effective means has been developed for specific detection of glutamate released from primary cultured neurons. Here we present a reduced graphene oxide (RGO)-based field effect transistor (FET) biosensor functionalized with synthesized glutamate receptor for real-time monitoring of glutamate release from prima...
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#1Xin Jin (Hubei University of Chinese Medicine)
#2Hong Zhang (Hubei University of Chinese Medicine)H-Index: 6
Last. Guo-Jun Zhang (Hubei University of Chinese Medicine)H-Index: 16
view all 9 authors...
The authors describe a field effect transistor (FET) based immunoassay for the detection of inactivated ebola virus (EBOV). An equine antibody against the EBOV glycoprotein was immobilized on the surface of the FET that was previously modified with reduced graphene oxide (RGO). The antibody against EBOV was immobilized on the modified FET, and the response to EBOV was measured as a function of the shift of Dirac voltage. The method can detect the EBOV over the concentration range from 2.4 × 10−1...
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