Jean-Claude Thomas
École Normale Supérieure
38Publications
20H-index
991Citations
Publications 38
Newest
Published on Apr 20, 2009in PLOS ONE 2.77
Lu-Ning Liu19
Estimated H-index: 19
(Leiden University),
Thijs J. Aartsma35
Estimated H-index: 35
(Leiden University)
+ 2 AuthorsBai-Cheng Zhou28
Estimated H-index: 28
(Shandong University)
Background Phycobilisomes (PBsomes) are the extrinsic antenna complexes upon the photosynthetic membranes in red algae and most cyanobacteria. The PBsomes in the cyanobacteria has been proposed to present high lateral mobility on the thylakoid membrane surface. In contrast, direct measurement of PBsome motility in red algae has been lacking so far. Methodology/Principal Findings In this work, we investigated the dynamics of PBsomes in the unicellular red alga Porphyridium cruentum in vivo and in...
15 Citations Source Cite
Published on Apr 3, 2009in Journal of Biological Chemistry 4.01
Nicolas Blot14
Estimated H-index: 14
,
Xian-Jun Wu2
Estimated H-index: 2
+ 10 AuthorsLutz A. Eichacker36
Estimated H-index: 36
Abstract Most cyanobacteria harvest light with large antenna complexes called phycobilisomes. The diversity of their constituting phycobiliproteins contributes to optimize the photosynthetic capacity of these microorganisms. Phycobiliprotein biosynthesis, which involves several post-translational modifications including covalent attachment of the linear tetrapyrrole chromophores (phycobilins) to apoproteins, begins to be well understood. However, the biosynthetic pathway to the blue-green-absorb...
53 Citations Source Cite
Published on Sep 4, 2008in PLOS ONE 2.77
Lu-Ning Liu19
Estimated H-index: 19
(Leiden University),
Abdalmohsen T. Elmalk3
Estimated H-index: 3
(Leiden University)
+ 4 AuthorsBai-Cheng Zhou28
Estimated H-index: 28
(Shandong University)
Background Photosynthetic organisms have developed multiple protective mechanisms to prevent photodamage in vivo under high-light conditions. Cyanobacteria and red algae use phycobilisomes (PBsomes) as their major light-harvesting antennae complexes. The orange carotenoid protein in some cyanobacteria has been demonstrated to play roles in the photoprotective mechanism. The PBsome-itself-related energy dissipation mechanism is still unclear. Methodology/Principal Findings Here, single-molecule s...
32 Citations Source Cite
Published on Dec 12, 2008in Journal of Biological Chemistry 4.01
Lu-Ning Liu19
Estimated H-index: 19
,
Thijs J. Aartsma35
Estimated H-index: 35
+ 3 AuthorsBai-Cheng Zhou28
Estimated H-index: 28
The architecture of the entire photosynthetic membrane network determines, at the supramolecular level, the physiological roles of the photosynthetic protein complexes involved. So far, a precise picture of the native configuration of red algal thylakoids is still lacking. In this work, we investigated the supramolecular architectures of phycobilisomes (PBsomes) and native thylakoid membranes from the unicellular red alga Porphyridium cruentum using atomic force microscopy (AFM) and transmission...
25 Citations Source Cite
Published on Jan 1, 2007in Current Microbiology 1.37
Gérald Zabulon13
Estimated H-index: 13
(École Normale Supérieure),
Catherine Richaud6
Estimated H-index: 6
(École Normale Supérieure)
+ 1 AuthorsJean-Claude Thomas20
Estimated H-index: 20
(École Normale Supérieure)
Cyanobacteria respond to nutrient-limiting conditions by degrading their phycobilisomes (PBS), the light-harvesting complexes for photosynthesis. In Synechococcus sp. PCC 7942, the expression of nblA, an essential gene in this process, is controlled by the response regulator NblR and the sensor NblS. Here we study the effect of inactivation of dspA (an nblS homologue) and an nblR-like gene on phycobilisome degradation in Synechocystis sp. PCC 6803, under nitrogen starvation. In each mutant, the ...
14 Citations Source Cite
Published on Jan 1, 2007in Genome Biology 13.21
Christophe Six10
Estimated H-index: 10
(University of Paris),
Jean-Claude Thomas20
Estimated H-index: 20
(École Normale Supérieure)
+ 5 AuthorsFrédéric Partensky47
Estimated H-index: 47
(University of Paris)
Background Marine Synechococcus owe their specific vivid color (ranging from blue-green to orange) to their large extrinsic antenna complexes called phycobilisomes, comprising a central allophycocyanin core and rods of variable phycobiliprotein composition. Three major pigment types can be defined depending on the major phycobiliprotein found in the rods (phycocyanin, phycoerythrin I or phycoerythrin II). Among strains containing both phycoerythrins I and II, four subtypes can be distinguished b...
138 Citations Source Cite
Published on May 1, 2006in Journal of Bacteriology 3.22
Craig Everroad1
Estimated H-index: 1
(University of Oregon),
Christophe Six10
Estimated H-index: 10
(Centre national de la recherche scientifique)
+ 3 AuthorsA. Michelle Wood20
Estimated H-index: 20
(University of Oregon)
Chromatic adaptation (CA) in cyanobacteria has provided a model system for the study of the environmental control of photophysiology for several decades. All forms of CA that have been examined so far (types II and III) involve changes in the relative contents of phycoerythrin (PE) and/or phycocyanin when cells are shifted from red to green light and vice versa. However, the chromophore compositions of these polypeptides are not altered. Some marine Synechococcus species strains, which possess t...
62 Citations Source Cite
Published on Mar 1, 2005in Journal of Bacteriology 3.22
Christophe Six5
Estimated H-index: 5
(Pierre-and-Marie-Curie University),
Jean-Claude Thomas20
Estimated H-index: 20
(École Normale Supérieure)
+ 3 AuthorsFrédéric Partensky47
Estimated H-index: 47
(Pierre-and-Marie-Curie University)
The recent availability of the whole genome of Synechococcus sp. strain WH8102 allows us to have a global view of the complex structure of the phycobilisomes of this marine picocyanobacterium. Genomic analyses revealed several new characteristics of these phycobilisomes, consisting of an allophycocyanin core and rods made of one type of phycocyanin and two types of phycoerythrins (I and II). Although the allophycocyanin appears to be similar to that found commonly in freshwater cyanobacteria, th...
40 Citations Source Cite
Published on May 1, 2004in Microbiology 0.85
Jesús A. G. Ochoa de Alda9
Estimated H-index: 9
(École Normale Supérieure),
Christiane Lichtlé12
Estimated H-index: 12
(École Normale Supérieure)
+ 1 AuthorsJean Houmard33
Estimated H-index: 33
(École Normale Supérieure)
In unicellular non-diazotrophic cyanobacteria, NblA is a small polypeptide required for phycobilisome degradation during macronutrient limitation. In the filamentous N2-fixing Tolypothrix sp., a nblA gene (nblAI) lies upstream of the cpeBA operon that encodes phycoerythrin apoproteins. Using a specific anti-NblAI antibody it was found that in strains of Tolypothrix sp. NblAI abundance increases under nitrogen-limiting conditions but the protein is also present in cells grown in nitrogen-replete ...
9 Citations Source Cite
Published on Oct 9, 2003in Molecular Microbiology 3.82
Ignacio Luque14
Estimated H-index: 14
(École Normale Supérieure),
Jesús A. G. Ochoa de Alda9
Estimated H-index: 9
(École Normale Supérieure)
+ 3 AuthorsJean Houmard33
Estimated H-index: 33
Summary Cyanobacteria respond to changes in light or nutrient availability by modifications in their photosynthetic light harvesting antenna. In unicellular cyanobacteria a small polypeptide (NblA) is required for phycobilisome degradation following environmental stresses. In the filamentous strain Tolypothrix sp. PCC 7601 the nblAI gene, encoding a NblA homologue, is located upstream of the operon coding for phycoerythrin (cpeBA). The nblAI transcripts all originate from a single transcription ...
29 Citations Source Cite
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