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Xinde Hu
Chinese Academy of Sciences
GeneGenome editingGeneticsBiologyCRISPR
16Publications
9H-index
293Citations
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Publications 17
Newest
#1Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
#2Xinde Hu (CAS: Chinese Academy of Sciences)H-Index: 9
Last. Xiaodong Sun (SJTU: Shanghai Jiao Tong University)H-Index: 1
view all 12 authors...
The smallest Cas13 family protein, CasRx, has a high cleavage activity and targeting specificity, offering attractive opportunity for therapeutic applications. Here we report that delivery of CasRx by adeno-associated virus via intravitreal injection could efficiently knockdown Vegfa transcripts and significantly reduce the area of laser-induced choroidal neovascularization in a mouse model of age-related macular degeneration. Thus, RNA-targeting CRISPR system could be used for in vivo gene ther...
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#1Xinde Hu (CAS: Chinese Academy of Sciences)H-Index: 9
#2Jinghan Wang (Fudan University)
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 10 authors...
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#1Jin-Jing Li (Fujian Medical University)H-Index: 2
#2Xiang Lin (Fujian Medical University)H-Index: 3
Last. Wan-Jin Chen (Fujian Medical University)H-Index: 11
view all 24 authors...
Source
#1Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
#2Yidi Sun (CAS: Chinese Academy of Sciences)H-Index: 8
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 14 authors...
Recently developed DNA base editing methods enable the direct generation of desired point mutations in genomic DNA without generating any double-strand breaks1–3, but the issue of off-target edits has limited the application of these methods. Although several previous studies have evaluated off-target mutations in genomic DNA4–8, it is now clear that the deaminases that are integral to commonly used DNA base editors often bind to RNA9–13. For example, the cytosine deaminase APOBEC1—which is used...
16 CitationsSource
#1Xuan Yao (CAS: Chinese Academy of Sciences)H-Index: 8
#2Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 5
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 16 authors...
Summary The targeting efficiency of knockin sequences via homologous recombination (HR) is generally low. Here we describe a method we call Tild-CRISPR (targeted integration with linearized dsDNA-CRISPR), a targeting strategy in which a PCR-amplified or precisely enzyme-cut transgene donor with 800-bp homology arms is injected with Cas9 mRNA and single guide RNA into mouse zygotes. Compared with existing targeting strategies, this method achieved much higher knockin efficiency in mouse embryos, ...
19 CitationsSource
#1Wenxin Yu (SJTU: Shanghai Jiao Tong University)H-Index: 5
#2Jiafang Zhu (SJTU: Shanghai Jiao Tong University)H-Index: 2
Last. Xiaoxi Lin (SJTU: Shanghai Jiao Tong University)H-Index: 9
view all 13 authors...
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#1Haibo Zhou (CAS: Chinese Academy of Sciences)H-Index: 3
#2J.Q. Liu (CAS: Chinese Academy of Sciences)H-Index: 4
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 22 authors...
Despite rapid progresses in the genome-editing field, in vivo simultaneous overexpression of multiple genes remains challenging. We generated a transgenic mouse using an improved dCas9 system that enables simultaneous and precise in vivo transcriptional activation of multiple genes and long noncoding RNAs in the nervous system. As proof of concept, we were able to use targeted activation of endogenous neurogenic genes in these transgenic mice to directly and efficiently convert astrocytes into f...
44 CitationsSource
#1Erwei Zuo (CAS: Chinese Academy of Sciences)H-Index: 6
#2Xiaona Huo (CAS: Chinese Academy of Sciences)H-Index: 2
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 18 authors...
The CRISPR/Cas9 system has become an efficient gene editing method for generating cells carrying precise gene mutations, including the rearrangement and deletion of chromosomal segments. However, whether an entire chromosome could be eliminated by this technology is still unknown. Here we demonstrate the use of the CRISPR/Cas9 system to eliminate targeted chromosomes. Using either multiple cleavages induced by a single-guide RNA (sgRNA) that targets multiple chromosome-specific sites or a cockta...
34 CitationsSource
#1Erwei Zuo (CAS: Chinese Academy of Sciences)H-Index: 6
#2Yijun Cai (CAS: Chinese Academy of Sciences)H-Index: 8
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 29 authors...
One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs
47 CitationsSource
#1Xuan Yao (CAS: Chinese Academy of Sciences)H-Index: 8
#2Xing Wang (CAS: Chinese Academy of Sciences)H-Index: 10
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 11 authors...
Abstract Precisely targeted genome editing is highly desired for clinical applications. However, the widely used homology-directed repair (HDR)-based genome editing strategies remain inefficient for certain in vivo applications. We here demonstrate a microhomology-mediated end-joining (MMEJ)-based strategy for precisely targeted gene integration in transfected neurons and hepatocytes in vivo with efficiencies up to 20%, much higher (up to 10 fold) than HDR-based strategy in adult mouse tissues. ...
21 CitationsSource
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