Peter Eberhardt
Goethe University Frankfurt
Infrared spectroscopyChemistryRhodopsinSodiumIon transporter
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Publications 4
#1Jagdeep Kaur (Goethe University Frankfurt)H-Index: 4
#2Clara Nassrin Kriebel (Goethe University Frankfurt)H-Index: 2
Last. Clemens Glaubitz (Goethe University Frankfurt)H-Index: 33
view all 12 authors...
Abstract Krokinobacter eikastus rhodopsin 2 (KR2) is a pentameric, light-driven ion pump, which selectively transports sodium or protons. The mechanism of ion selectivity and transfer is unknown. By using conventional as well as dynamic nuclear polarization (DNP)-enhanced solid-state NMR, we were able to analyse the retinal polyene chain between positions C10 and C15 as well as the Schiff base nitrogen in the KR2 resting state. In addition, 50% of the KR2 13C and 15N resonances could be assigned...
9 CitationsSource
#1Marvin Asido (Goethe University Frankfurt)H-Index: 1
#2Peter Eberhardt (Goethe University Frankfurt)H-Index: 3
Last. Josef Wachtveitl (Goethe University Frankfurt)H-Index: 38
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We report a comparative study on the structural dynamics of the light-driven sodium pump Krokinobacter eikastus rhodopsin 2 wild type under sodium and proton pumping conditions by means of time-resolved IR spectroscopy. The kinetics of KR2 under sodium pumping conditions exhibits a sequential character, whereas the kinetics of KR2 under proton pumping conditions involves several equilibrium states. The sodium translocation itself is characterized by major conformational changes of the protein ba...
2 CitationsSource
#1Elena Bühl (Goethe University Frankfurt)H-Index: 2
#2Peter Eberhardt (Goethe University Frankfurt)H-Index: 3
Last. Josef Wachtveitl (Goethe University Frankfurt)H-Index: 38
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Ultrafast infrared transient absorption in the carbonyl vibrational region of protonated aspartate and glutamate residues in channelrhodopsin-2 from Chlamydomonas reinhardtii shows immediate protein response to retinal excitation. The observed difference bands are formed directly after the excitation on the subpicosecond time scale and were assigned to side chains in the retinal vicinity, such as D156 and E90. This finding implies an ultrafast and effective energy transfer from the retinal to it...
3 CitationsSource
#1Erik Henrich (Goethe University Frankfurt)H-Index: 9
#2Janina Sörmann (MPG: Max Planck Society)H-Index: 1
Last. Christian Bamann (MPG: Max Planck Society)H-Index: 22
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Abstract Nanodiscs that hold a lipid bilayer surrounded by a boundary of scaffold proteins have emerged as a powerful tool for membrane protein solubilization and analysis. By combining nanodiscs and cell-free expression technologies, even completely detergent-free membrane protein characterization protocols can be designed. Nanodiscs are compatible with various techniques, and due to their bilayer environment and increased stability, they are often superior to detergent micelles or liposomes fo...
8 CitationsSource