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Frédéric Fischer
Centre national de la recherche scientifique
7Publications
5H-index
107Citations
Publications 7
Newest
#1Sophie Scheidecker (UDS: University of Strasbourg)H-Index: 7
#2Sophie Scheidecker (French Institute of Health and Medical Research)
Last.Hélène Dollfus (French Institute of Health and Medical Research)H-Index: 52
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Mutations in genes encoding aaRSs (aminoacyl-tRNA synthetases) have been reported in several neurological disorders. KARS is a dual localized lysyl-tRNA synthetase (LysRS) and its cytosolic isoform belongs to the multiple aminoacyl-tRNA synthetase complex (MSC). Biallelic mutations in KARS gene were described in a wide phenotypic spectrum ranging from non-syndromic deafness to complex impairments. Here, we report on a patient with severe neurological and neurosensory disease investigated by whol...
#1Nathaniel Yakobov (UDS: University of Strasbourg)H-Index: 1
#2Sylvain Debard (UDS: University of Strasbourg)H-Index: 2
Last.Hubert Dominique Becker (UDS: University of Strasbourg)H-Index: 24
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Abstract Prokaryotic and eukaryotic cytosolic aminoacyl-tRNA synthetases (aaRSs) are essentially known for their conventional function of generating the full set of aminoacyl-tRNA species that are needed to incorporate each organism's repertoire of genetically-encoded amino acids during ribosomal translation of messenger RNAs. However, bacterial and eukaryotic cytosolic aaRSs have been shown to exhibit other essential nonconventional functions. Here we review all the subcellular compartments tha...
#1Yuhei Araiso (CNRS: Centre national de la recherche scientifique)H-Index: 7
#2Jonathan L. Huot (CNRS: Centre national de la recherche scientifique)H-Index: 4
Last.Osamu Nureki (UTokyo: University of Tokyo)H-Index: 53
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Yeast mitochondrial Gln-mtRNAGln is synthesized by the transamidation of mischarged Glu-mtRNAGln by a non-canonical heterotrimeric tRNA-dependent amidotransferase (AdT). The GatA and GatB subunits of the yeast AdT (GatFAB) are well conserved among bacteria and eukaryota, but the GatF subunit is a fungi-specific ortholog of the GatC subunit found in all other known heterotrimeric AdTs (GatCAB). Here we report the crystal structure of yeast mitochondrial GatFAB at 2.0 A resolution. The C-terminal ...
#1Gayathri Silva (WSU: Wayne State University)H-Index: 2
#2Shirin Fatma (WSU: Wayne State University)H-Index: 2
Last.Tamara L. Hendrickson (WSU: Wayne State University)H-Index: 16
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Abstract Many bacteria lack genes encoding asparaginyl- and/or glutaminyl-tRNA synthetase and consequently rely on an indirect path for the synthesis of both Asn-tRNAAsn and Gln-tRNAGln. In some bacteria such as Thermus thermophilus, efficient delivery of misacylated tRNA to the downstream amidotransferase (AdT) is ensured by formation of a stable, tRNA-dependent macromolecular complex called the Asn-transamidosome. This complex enables direct delivery of Asp-tRNAAsn from the non-discriminating ...
#1Bernard Lorber (UDS: University of Strasbourg)H-Index: 28
#2Frédéric Fischer (UDS: University of Strasbourg)H-Index: 5
Last.Daniel Kern (UDS: University of Strasbourg)H-Index: 33
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Dynamic light scattering (DLS) analyses are routinely used in biology laboratories to detect aggregates in macromolecular solutions, to determine the size of proteins, nucleic acids, and complexes or to monitor the binding of ligands. This article is written for graduate and undergraduate students with access to DLS and for faculty members who wish to incorporate DLS into a lab activity, a practical course or research. It reviews the basic concepts of light scattering measurements and addresses ...
#1Frédéric Fischer (UDS: University of Strasbourg)H-Index: 5
#2Jonathan L. Huot (UDS: University of Strasbourg)H-Index: 4
Last.Daniel Kern (UDS: University of Strasbourg)H-Index: 33
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Helicobacter pylori catalyzes Asn-tRNAAsn formation by use of the indirect pathway that involves charging of Asp onto tRNAAsn by a non-discriminating aspartyl-tRNA synthetase (ND-AspRS), followed by conversion of the mischarged Asp into Asn by the GatCAB amidotransferase. We show that the partners of asparaginylation assemble into a dynamic Asn-transamidosome, which uses a different strategy than the Gln-transamidosome to prevent the release of the mischarged aminoacyl-tRNA intermediate. The com...
#1Mickael Blaise (AU: Aarhus University)H-Index: 16
#2Marc Bailly (UDS: University of Strasbourg)H-Index: 3
Last.Daniel Kern (UDS: University of Strasbourg)H-Index: 33
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Four out of the 22 aminoacyl-tRNAs (aa-tRNAs) are systematically or alternatively synthesized by an indirect, two-step route requiring an initial mischarging of the tRNA followed by tRNA-dependent conversion of the non-cognate amino acid. During tRNA-dependent asparagine formation, tRNAAsn promotes assembly of a ribonucleoprotein particle called transamidosome that allows channelling of the aa-tRNA from non-discriminating aspartyl-tRNA synthetase active site to the GatCAB amidotransferase site. ...
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