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Kenya Sato
Central Institute for Experimental Animals
4Publications
3H-index
171Citations
Publications 4
Newest
#1Wakako Kumita (Central Institute for Experimental Animals)H-Index: 2
#2Kenya Sato (Central Institute for Experimental Animals)H-Index: 3
Last.Erika Sasaki (Central Institute for Experimental Animals)H-Index: 21
view all 13 authors...
Genetically modified nonhuman primates (NHP) are useful models for biomedical research. Gene editing technologies have enabled production of target-gene knock-out (KO) NHP models. Target-gene-KO/knock-in (KI) efficiency of CRISPR/Cas9 has not been extensively investigated in marmosets. In this study, optimum conditions for target gene modification efficacies of CRISPR/mRNA and CRISPR/nuclease in marmoset embryos were examined. CRISPR/nuclease was more effective than CRISPR/mRNA in avoiding mosai...
1 CitationsSource
#1Kenya Sato (Central Institute for Experimental Animals)H-Index: 3
#2Ryo Oiwa (Central Institute for Experimental Animals)H-Index: 5
Last.Erika Sasaki (Central Institute for Experimental Animals)H-Index: 21
view all 26 authors...
Recent advances in genome editing have facilitated the generation of nonhuman primate (NHP) models, with potential to unmask the complex biology of human disease not revealed by rodent models. However, their broader use is hindered by the challenges associated with generation of adult NHP models as well as the cost of their production. Here, we describe the generation of a marmoset model of severe combined immunodeficiency (SCID). This study optimized zinc-finger nucleases (ZFNs) and transcripti...
55 CitationsSource
#1Tsukasa Takahashi (Central Institute for Experimental Animals)H-Index: 1
#2Kisaburo Hanazawa (Juntendo University)H-Index: 11
Last.Erika Sasaki (Central Institute for Experimental Animals)H-Index: 21
view all 14 authors...
Intracytoplasmic sperm injection (ICSI), an important method used to treat male subfertility, is applied in the transgenic technology of sperm-mediated gene transfer. However, no study has described successful generation of offspring using ICSI in the common marmoset, a small non-human primate used as a model for biomedical translational research. In this study, we investigated blastocyst development and the subsequent live offspring stages of marmoset oocytes matured in vitro and fertilized by ...
16 CitationsSource
#1Noriyuki Kishi (Keio: Keio University)H-Index: 11
#2Kenya Sato (Central Institute for Experimental Animals)H-Index: 3
Last.Hideyuki Okano (Keio: Keio University)H-Index: 112
view all 4 authors...
The common marmoset (Callithrix jacchus) is a small New World primate; it originally comes from the Atlantic coastal forests in northeastern Brazil. It has been attracting much attention in the biomedical research field because of its size, availability, and unique biological characteristics. Its endocrinological and behavioral similarity to humans, comparative ease in handling, and high reproductive efficiency are very advantageous for neuroscience research. Recently, we developed transgenic co...
99 CitationsSource
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