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Raees Khan
Dong-a University
11Publications
4H-index
37Citations
Publications 11
Newest
#1Raees Khan (Dong-a University)H-Index: 4
#2Amir Zeb (Gyeongsang National University)H-Index: 5
Last.Seon-Woo Lee (Dong-a University)H-Index: 12
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Enoyl-acyl carrier protein reductase (ENR) catalyzes the last reduction step in the bacterial type II fatty acid biosynthesis cycle. ENRs include FabI, FabL, FabL2, FabK, and FabV. Previously, we reported a unique triclosan (TCL) resistant ENR homolog that was predominant in obligate intracellular pathogenic bacteria and Apicomplexa. Herein, we report the biochemical and structural basis of TCL resistance in this novel ENR. The purified protein revealed NADH-dependent ENR activity and shared sim...
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#1Raees KhanH-Index: 4
#2Amir ZebH-Index: 5
Last.Seon-Woo LeeH-Index: 12
view all 7 authors...
1 CitationsSource
#1Raees Khan (Dong-a University)H-Index: 4
#2Muhammad Israr Khan (QAU: Quaid-i-Azam University)H-Index: 1
Last.Zuhaibuddin Bhutto (BUET: Bangladesh University of Engineering and Technology)H-Index: 2
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Extremophiles, the microorganisms thriving in extreme environments, provide valuable resources for practicing novel biotechnological processes. Pakistan homes a wide spectrum of extreme environments which harbor various biotechnologically significant microorganisms. This review gauges the structural and functional bacterial diversity of several extreme environments, emphasizing their potentials as a source of extremozymes, and in bioleaching, bioremediation, and bioenergy production at regional ...
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#1Myung Hwan Lee (Dong-a University)H-Index: 9
#2Raees Khan (Dong-a University)H-Index: 4
Last.Seon-Woo Lee (Dong-a University)H-Index: 12
view all 8 authors...
Abstract Autoinducers are indispensable for bacterial cell–cell communication. However, due to the reliance on culture-based techniques, few autoinducer-hydrolyzing enzymes are known. In this study, we characterized soil metagenome-derived unique enzymes capable of hydrolyzing 3-hydroxypalmitic acid methyl ester (3-OH PAME), an autoinducer of the plant pathogenic bacterium Ralstonia solanacearum. Among 146 candidate lipolytic clones from a soil metagenome library, 4 unique enzymes capable of hyd...
3 CitationsSource
#1Raees Khan (Dong-a University)H-Index: 4
#2Nazish Roy (Dong-a University)H-Index: 3
Last.Seon-Woo Lee (Dong-a University)H-Index: 12
view all 4 authors...
The substantial use of triclosan (TCS) has been aimed to kill pathogenic bacteria, but TCS resistance seems to be prevalent in microbial species and limited knowledge exists about TCS resistance determinants in a majority of pathogenic bacteria. We aimed to evaluate the distribution of TCS resistance determinants in major pathogenic bacteria (N = 231) and to assess the enrichment of potentially pathogenic genera in TCS contaminated environments. A TCS-resistant gene (TRG) database was constructe...
7 CitationsSource
1 CitationsSource
#1Raees KhanH-Index: 4
#2Hyun Gi KongH-Index: 8
Last.Seon-Woo LeeH-Index: 12
view all 7 authors...
Triclosan (TCS) is a widely used antimicrobial agent and TCS resistance is considered to have evolved in diverse organisms with extensive use of TCS, but distribution of TCS resistance has not been well characterized. Functional screening of the soil metagenome in this study has revealed that a variety of target enoyl acyl carrier protein reductases (ENR) homologues are responsible for the majority of TCS resistance. Diverse ENRs similar to 7-α-hydroxysteroid dehydrogenase (7-α-HSDH), FabG, or t...
12 CitationsSource
#1Shabir Ahmad (Dong-a University)H-Index: 2
#2Seung Yeup Lee (Dong-a University)H-Index: 3
Last.Seon-Woo Lee (Dong-a University)H-Index: 12
view all 7 authors...
Ralstonia solanacearum is a soil-borne plant pathogen that infects more than 200 plant species. Its broad host range and long-term survival under different environmental stress conditions suggest that it uses a variety of mechanisms to protect itself against various types of biotic and abiotic stress. R. solanacearum produces a melanin-like brown pigment in the stationary phase when grown in minimal medium containing tyrosine. To gain deeper insight into the genetic determinants involved in mela...
8 CitationsSource
Triclosan, the widely used biocide, specifically targets enoyl-acyl carrier protein reductase (ENR) in the bacterial fatty acid synthesis system. Although the fish pathogen Aeromonas salmonicida subsp. salmonicida exhibits triclosan resistance, the nature of this resistance has not been elucidated. Here, we aimed to characterize the triclosan resistance of A. salmonicida subsp. salmonicida causing furunculosis. The fosmid library of triclosan-resistant A. salmonicida subsp. salmonicida was const...
5 CitationsSource
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