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L.L. Clancy
Upjohn
Crystal structureChemistryCrystallizationInterleukinCrystallography
9Publications
5H-index
234Citations
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Publications 4
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#1L.L. Clancy (Upjohn)H-Index: 5
#2G.S.J. Rao (Texas College)H-Index: 2
Last. H.M. Einspahr (Upjohn)H-Index: 6
view all 11 authors...
Abstract The malic enzyme from muscle mitochondria of the parasitic nematode Ascaris suum is a tetramer of 65 kDa monomers that catalyzes the oxidative decarboxylation of malate to pyruvate and CO 2 with NAD cofactor as oxidant. This malic enzyme is critical to the nematode for muscle function under anaerobic conditions. Unlike mammalian versions of the enzyme such as that found in rat liver, which require NADP as cofactor, the nematode version is an NAD-dependent enzyme. We report the crystalli...
8 CitationsSource
#1Barry C. Finzel (Upjohn)H-Index: 23
#2L.L. Clancy (Upjohn)H-Index: 5
Last. H.M. Einspahr (Upjohn)H-Index: 6
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The crystal structure of recombinant human interleukin-1β (IL-1β) has been determined at 2·0 A resolution and refined to a crystallographic R-factor of 0·19. Three heavy-atom derivatives were identified and used for multiple isomorphous replacement phasing. Interpretation of the resulting electron density map revealed a structure in which there are 12 antiparallel β-strands and no α-helix. The single 153-residue polypeptide chain is folded into a six-stranded β-barrel similar in architecture to ...
141 CitationsSource
#1H.M. Einspahr (Upjohn)H-Index: 6
#2L.L. Clancy (Upjohn)H-Index: 5
Last. Robert L. Heinrikson (Upjohn)H-Index: 31
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Abstract The gene for the fully processed form of human interleukin 1β was cloned from SK-hep-1 hepatoma cellular RNA and expressed at high levels in E. coli . The protein produced in E. coli . was purified to homogeneity by standard chromatographic methods, including adsorption and desorption from Procion Red Sepharose, sizing on a Superose 12 FPLC column, and anion exchange chromatography on QAE Sepharose. The result is a biologically active protein, rIL-1β, that migrates on two-dimensional ge...
7 CitationsSource
#1Donald B. Carter (Upjohn)H-Index: 23
#2K. A. Curry (Upjohn)H-Index: 3
Last. H.M. Einspahr (Upjohn)H-Index: 6
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The gene for human interleukin-1β was cloned from SK-hep-1 hepatoma cellular RNA and expressed at high levels in Escherichia coli both as the naturally processed form (rIL-1β) and as a variant with an additional sequence of three amino acids on the N-terminus (rIL-1β+). Expressed protein was purified to homogeneity by a sequence of steps, which included low pH incubation, adsorption and desorption from Procion Red Sepharose, sizing on a Superose 12 fast-performance liquid chromatography (FPLC) c...
37 CitationsSource
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